Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,451,037 T→C 8.3% T804A (ACC→GCC)  PP_3069 ← outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,451,0370TC 8.3% 134.2 / 2.9 48T804A (ACC→GCC) PP_3069outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base T (24/20);  new base C (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

CCGGCCCACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGT  >  NC_002947/3450890‑3451180
                                                                                                                                                   |                                                                                                                                               
ccGGCCCACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGgtg                                                                                                                                                >  2:232031/1‑149 (MQ=255)
    cccACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACt                                                                                                                                            >  7:153661/1‑149 (MQ=255)
      cACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTg                                                                                                                                           >  6:195406/1‑148 (MQ=255)
       aCGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCa                                                                                                                                         >  1:127315/1‑149 (MQ=255)
                 ttCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATg                                                                                                                               <  6:270904/149‑1 (MQ=255)
                   cAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGCGTGCAGTACACCGCCaacc                                                                                                                              <  1:191562/148‑4 (MQ=255)
                     aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                           <  8:153661/149‑1 (MQ=255)
                     aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                           <  8:342986/149‑1 (MQ=255)
                           tCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAgc                                                                                                                     <  7:231593/149‑1 (MQ=255)
                                gcgTCCGTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTGCCTGCGGGCGGCTGGCCAGGGTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTc                                                                                                                <  6:53801/149‑1 (MQ=255)
                                   tCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGtt                                                                                                             <  5:195406/149‑1 (MQ=255)
                                     cTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGa                                                                                                           >  7:341566/1‑149 (MQ=255)
                                      ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                          >  2:307466/1‑149 (MQ=255)
                                      ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                          >  6:193033/1‑149 (MQ=255)
                                          tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                      >  5:138594/1‑149 (MQ=255)
                                          tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                      >  2:152070/1‑149 (MQ=255)
                                                  gcAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACca                                                                                               >  6:252137/1‑148 (MQ=255)
                                                     ggTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACAcc                                                                                           <  6:139889/149‑1 (MQ=255)
                                                           ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                    >  7:83460/1‑134 (MQ=255)
                                                           ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                    <  8:83460/134‑1 (MQ=255)
                                                           ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                     <  5:80088/149‑1 (MQ=255)
                                                           ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                     <  6:242746/149‑1 (MQ=255)
                                                                gctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                >  5:59249/1‑149 (MQ=255)
                                                                 ttggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAg                                                                                  <  4:354930/145‑1 (MQ=255)
                                                                 ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                <  7:338011/148‑1 (MQ=255)
                                                                 ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                <  8:220016/148‑1 (MQ=255)
                                                                 ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTAGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                               >  8:325848/1‑149 (MQ=255)
                                                                   ggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                               >  3:219842/1‑147 (MQ=255)
                                                                    gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTTGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGc                                                                              <  7:325848/147‑1 (MQ=255)
                                                                    gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCAc                                                                            <  5:193033/149‑1 (MQ=255)
                                                                        cgcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGa                                                                         <  5:252137/148‑1 (MQ=255)
                                                                         gcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAgg                                                                       >  2:264220/1‑149 (MQ=255)
                                                                             cgcTAGATCTGCCAGCGCACTCCCAGGTTCACGCCGCTGGGTTCCTGCTGGTGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTTACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGtata                                                                   <  4:241724/149‑1 (MQ=255)
                                                                               cTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCGTTCCAGTTGCACTGAGGTATAGc                                                                 >  5:91305/1‑149 (MQ=255)
                                                                                   aaCTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGt                                                              >  6:313438/1‑148 (MQ=255)
                                                                                               aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                 >  2:285232/1‑149 (MQ=255)
                                                                                               aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                 <  8:341566/149‑1 (MQ=255)
                                                                                                 gCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCCGCAGTCCAGGTTGGCGCTGTACTCCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTAGCACAGAGGTATACCGGTAATCGGTCTTGAtat                                               >  2:272693/1‑147 (MQ=255)
                                                                                                   ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCGAGGTTGGCGGTGTACTGCCCGCCGCCATGCAGGCTCCGCGCTTCGTTGAGCTGCGCTACCACACAGCTTTCCAGTTGCGCTGAGGTATAGCGGTATTCGGTCTTGATCTTg                                             >  8:99950/1‑149 (MQ=255)
                                                                                                   ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTg                                             >  8:104810/1‑149 (MQ=255)
                                                                                                         tCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCg                                       >  8:68500/1‑149 (MQ=255)
                                                                                                                         ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAgg                       >  6:263335/1‑149 (MQ=255)
                                                                                                                         ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAg                        <  5:64236/148‑1 (MQ=255)
                                                                                                                                 ggcTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCgcgc               >  3:195587/1‑149 (MQ=255)
                                                                                                                                        cAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGt        >  7:281460/1‑149 (MQ=255)
                                                                                                                                         tgcatGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTc       >  3:347141/5‑149 (MQ=255)
                                                                                                                                            atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                 >  6:369543/2‑88 (MQ=38)
                                                                                                                                            atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                 <  5:369543/87‑1 (MQ=38)
                                                                                                                                               gCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGt  <  6:59249/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCGGCCCACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGT  >  NC_002947/3450890‑3451180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑