Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,451,040 A→G 8.3% Y803H (TAC→CAC)  PP_3069 ← outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,451,0400AG 8.3% 130.3 / 3.2 48Y803H (TAC→CAC) PP_3069outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base A (24/20);  new base G (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CCCACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGT  >  NC_002947/3450894‑3451180
                                                                                                                                                  |                                                                                                                                            
cccACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACt                                                                                                                                            >  7:153661/1‑149 (MQ=255)
  cACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTg                                                                                                                                           >  6:195406/1‑148 (MQ=255)
   aCGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCa                                                                                                                                         >  1:127315/1‑149 (MQ=255)
             ttCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATg                                                                                                                               <  6:270904/149‑1 (MQ=255)
               cAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGCGTGCAGTACACCGCCaacc                                                                                                                              <  1:191562/148‑4 (MQ=255)
                 aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                           <  8:153661/149‑1 (MQ=255)
                 aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                           <  8:342986/149‑1 (MQ=255)
                       tCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAgc                                                                                                                     <  7:231593/149‑1 (MQ=255)
                            gcgTCCGTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTGCCTGCGGGCGGCTGGCCAGGGTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTc                                                                                                                <  6:53801/149‑1 (MQ=255)
                               tCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGtt                                                                                                             <  5:195406/149‑1 (MQ=255)
                                 cTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGa                                                                                                           >  7:341566/1‑149 (MQ=255)
                                  ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                          >  2:307466/1‑149 (MQ=255)
                                  ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                          >  6:193033/1‑149 (MQ=255)
                                      tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                      >  5:138594/1‑149 (MQ=255)
                                      tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                      >  2:152070/1‑149 (MQ=255)
                                              gcAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACca                                                                                               >  6:252137/1‑148 (MQ=255)
                                                 ggTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACAcc                                                                                           <  6:139889/149‑1 (MQ=255)
                                                       ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                    >  7:83460/1‑134 (MQ=255)
                                                       ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                    <  8:83460/134‑1 (MQ=255)
                                                       ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                     <  6:242746/149‑1 (MQ=255)
                                                       ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                     <  5:80088/149‑1 (MQ=255)
                                                            gctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                >  5:59249/1‑149 (MQ=255)
                                                             ttggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAg                                                                                  <  4:354930/145‑1 (MQ=255)
                                                             ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                <  7:338011/148‑1 (MQ=255)
                                                             ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                <  8:220016/148‑1 (MQ=255)
                                                             ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTAGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                               >  8:325848/1‑149 (MQ=255)
                                                               ggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                               >  3:219842/1‑147 (MQ=255)
                                                                gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTTGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGc                                                                              <  7:325848/147‑1 (MQ=255)
                                                                gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCAc                                                                            <  5:193033/149‑1 (MQ=255)
                                                                    cgcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGa                                                                         <  5:252137/148‑1 (MQ=255)
                                                                     gcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAgg                                                                       >  2:264220/1‑149 (MQ=255)
                                                                         cgcTAGATCTGCCAGCGCACTCCCAGGTTCACGCCGCTGGGTTCCTGCTGGTGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTTACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGtata                                                                   <  4:241724/149‑1 (MQ=255)
                                                                           cTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCGTTCCAGTTGCACTGAGGTATAGc                                                                 >  5:91305/1‑149 (MQ=255)
                                                                               aaCTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGt                                                              >  6:313438/1‑148 (MQ=255)
                                                                                           aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                 <  8:341566/149‑1 (MQ=255)
                                                                                           aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                 >  2:285232/1‑149 (MQ=255)
                                                                                             gCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCCGCAGTCCAGGTTGGCGCTGTACTCCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTAGCACAGAGGTATACCGGTAATCGGTCTTGAtat                                               >  2:272693/1‑147 (MQ=255)
                                                                                               ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCGAGGTTGGCGGTGTACTGCCCGCCGCCATGCAGGCTCCGCGCTTCGTTGAGCTGCGCTACCACACAGCTTTCCAGTTGCGCTGAGGTATAGCGGTATTCGGTCTTGATCTTg                                             >  8:99950/1‑149 (MQ=255)
                                                                                               ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTg                                             >  8:104810/1‑149 (MQ=255)
                                                                                                     tCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCg                                       >  8:68500/1‑149 (MQ=255)
                                                                                                                     ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAgg                       >  6:263335/1‑149 (MQ=255)
                                                                                                                     ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAg                        <  5:64236/148‑1 (MQ=255)
                                                                                                                             ggcTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCgcgc               >  3:195587/1‑149 (MQ=255)
                                                                                                                                    cAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGt        >  7:281460/1‑149 (MQ=255)
                                                                                                                                     tgcatGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTc       >  3:347141/5‑149 (MQ=255)
                                                                                                                                        atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                 >  6:369543/2‑88 (MQ=38)
                                                                                                                                        atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                 <  5:369543/87‑1 (MQ=38)
                                                                                                                                           gCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGt  <  6:59249/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CCCACGCCGAATGTTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGT  >  NC_002947/3450894‑3451180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑