Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,451,047 G→A 7.8% G800G (GGC→GGT)  PP_3069 ← outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,451,0470GA 7.8% 147.8 / 2.5 51G800G (GGC→GGT) PP_3069outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

TTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGTGCCACAGGTTGAC  >  NC_002947/3450907‑3451193
                                                                                                                                            |                                                                                                                                                  
ttCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATg                                                                                                                                            <  6:270904/149‑1 (MQ=255)
  cAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGCGTGCAGTACACCGCCaacc                                                                                                                                           <  1:191562/148‑4 (MQ=255)
    aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                                        <  8:153661/149‑1 (MQ=255)
    aCCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAgg                                                                                                                                        <  8:342986/149‑1 (MQ=255)
          tCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAgc                                                                                                                                  <  7:231593/149‑1 (MQ=255)
               gcgTCCGTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTGCCTGCGGGCGGCTGGCCAGGGTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTc                                                                                                                             <  6:53801/149‑1 (MQ=255)
                  tCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGtt                                                                                                                          <  5:195406/149‑1 (MQ=255)
                    cTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGa                                                                                                                        >  7:341566/1‑149 (MQ=255)
                     ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                                       >  2:307466/1‑149 (MQ=255)
                     ttGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGAc                                                                                                                       >  6:193033/1‑149 (MQ=255)
                         tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                                   >  2:152070/1‑149 (MQ=255)
                         tGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTgc                                                                                                                   >  5:138594/1‑149 (MQ=255)
                                 gcAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACca                                                                                                            >  6:252137/1‑148 (MQ=255)
                                    ggTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACAcc                                                                                                        <  6:139889/149‑1 (MQ=255)
                                          ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                                 >  7:83460/1‑134 (MQ=255)
                                          ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCTTGCAGGCTCAGCGCTTCGTTGACCTgcgc                                                                                                                 <  8:83460/134‑1 (MQ=255)
                                          ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                                  <  5:80088/149‑1 (MQ=255)
                                          ggTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTc                                                                                                  <  6:242746/149‑1 (MQ=255)
                                               gctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                             >  5:59249/1‑149 (MQ=255)
                                                ttggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAg                                                                                               <  4:354930/145‑1 (MQ=255)
                                                ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                             <  7:338011/148‑1 (MQ=255)
                                                ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGtt                                                                                             <  8:220016/148‑1 (MQ=255)
                                                ctggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTAGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                                            >  8:325848/1‑149 (MQ=255)
                                                  ggctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTg                                                                                            >  3:219842/1‑147 (MQ=255)
                                                   gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTTGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGc                                                                                           <  7:325848/147‑1 (MQ=255)
                                                   gctgCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCAc                                                                                         <  5:193033/149‑1 (MQ=255)
                                                       cgcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGa                                                                                      <  5:252137/148‑1 (MQ=255)
                                                        gcgcCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAgg                                                                                    >  2:264220/1‑149 (MQ=255)
                                                            cgcTAGATCTGCCAGCGCACTCCCAGGTTCACGCCGCTGGGTTCCTGCTGGTGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTTACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGtata                                                                                <  4:241724/149‑1 (MQ=255)
                                                              cTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCGTTCCAGTTGCACTGAGGTATAGc                                                                              >  5:91305/1‑149 (MQ=255)
                                                                  aaCTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGt                                                                           >  6:313438/1‑148 (MQ=255)
                                                                              aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                              <  8:341566/149‑1 (MQ=255)
                                                                              aCGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGAt                                                              >  2:285232/1‑149 (MQ=255)
                                                                                gCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCCGCAGTCCAGGTTGGCGCTGTACTCCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTAGCACAGAGGTATACCGGTAATCGGTCTTGAtat                                                            >  2:272693/1‑147 (MQ=255)
                                                                                  ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCGAGGTTGGCGGTGTACTGCCCGCCGCCATGCAGGCTCCGCGCTTCGTTGAGCTGCGCTACCACACAGCTTTCCAGTTGCGCTGAGGTATAGCGGTATTCGGTCTTGATCTTg                                                          >  8:99950/1‑149 (MQ=255)
                                                                                  ccAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTg                                                          >  8:104810/1‑149 (MQ=255)
                                                                                        tCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCg                                                    >  8:68500/1‑149 (MQ=255)
                                                                                                        ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAgg                                    >  6:263335/1‑149 (MQ=255)
                                                                                                        ctgctgGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAg                                     <  5:64236/148‑1 (MQ=255)
                                                                                                                ggcTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCgcgc                            >  3:195587/1‑149 (MQ=255)
                                                                                                                       cAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGt                     >  7:281460/1‑149 (MQ=255)
                                                                                                                        tgcatGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTc                    >  3:347141/5‑149 (MQ=255)
                                                                                                                           atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                              >  6:369543/2‑88 (MQ=38)
                                                                                                                           atGGCGGCGTGCAGTACACCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGc                                                                              <  5:369543/87‑1 (MQ=38)
                                                                                                                              gCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGt               <  6:59249/148‑1 (MQ=255)
                                                                                                                                      cTGCACGCCGCCATGCTGGCGGAGCGCTTCGTTGACCTGCGCTACCACTCCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCTGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGTg              <  6:100130/141‑1 (MQ=255)
                                                                                                                                      cTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACAACGCATTCCAGTTGCACTGAGGAATAGCGGTAAACGGTCTTGATCTTGTCAGCGTCATCGAAGCTCAAGGAGTCGCGCCCACCCTAGCCGTGcatatcg       >  5:100130/1‑142 (MQ=255)
                                                                                                                                       tGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGTGCCACAGGtt     >  3:224999/1‑149 (MQ=255)
                                                                                                                                         aaCGCCGCAATGATGGCTATGGGATTGGTTGACCTGCGCTACAACACCGCTTTCCAGTTGCACTTAGGTATAGCGGTAATCGTTCTTGATCTTGTCAGCGTCAGCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGTGCCACAGGTTGa   <  1:264220/148‑1 (MQ=255)
                                                                                                                                          aCGCCGCCATGCAGGCTCAGCTCTTCGTTGACCTGCGCTACCACACCGCTTTCCTGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCTTCGTAGGTCAAGGTGTGGCGCCCACCGTCGCCGTGCCACAGGTTGAc  <  1:272693/149‑1 (MQ=255)
                                                                                                                                          aCGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTTAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTTGCCGTGCCACAGGTTGAc  <  1:285232/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TTCAACCGGGTCAGCGCGTCCTTGCTGGGCTGCGCAGGTCTCGGTCTGCTGGCTGCGCGCCGCTAGAACTGCCAGCGCACGCCCAGGTTCACGCCGCTGGCTTCCTGCTGGCGGCTGTCCAGGTTGGCGGTGTACTGCACGCCGCCATGCAGGCTCAGCGCTTCGTTGACCTGCGCTACCACACCGCTTTCCAGTTGCACTGAGGTATAGCGGTAATCGGTCTTGATCTTGTCAGCGTCATCGAAGGTCAAGGTGTCGCGCCCACCGTCGCCGTGCCACAGGTTGAC  >  NC_002947/3450907‑3451193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑