Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,466,056	A→T	10.8%	noncoding (104/119 nt)	PP_mr39 ←	P15 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,466,056	0	A	T	10.8%	195.9 / 11.8	74	noncoding (104/119 nt)	PP_mr39	P15 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (35/31);  new base T (6/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

ATCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCG  >  NC_002947/3465909‑3466200                                                                                                                                                    |                                                                                                                                                 aTCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGaa                                                                                                                                                 <  5:109375/149‑1 (MQ=255)    ggAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGc                                                                                                                                               <  7:165884/148‑1 (MQ=255)            cAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCgggg                                                                                                                                      <  7:69374/149‑1 (MQ=255)                cTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTGTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                  <  7:233142/149‑1 (MQ=255)                 tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                  >  4:119716/1‑148 (MQ=255)                    cccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGt                                                                                                                              >  2:189754/1‑149 (MQ=255)                    cccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgtt                                                                                                                              >  1:173831/1‑145 (MQ=39)                     ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgtta                                                                                                                             >  5:31982/1‑144 (MQ=39)                     ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgt                                                                                                                               <  6:31982/147‑4 (MQ=39)                          cGGGTTTGGCTAAATCGCCAGGCGTTTGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATtctc                                                                                                                        <  2:9271/149‑1 (MQ=255)                           gggATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCa                                                                                                                       >  3:34415/1‑149 (MQ=255)                            ggATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      <  2:107713/149‑1 (MQ=255)                              aTTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGt                                                                                                                    >  2:63614/1‑149 (MQ=255)                                  gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAATACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                >  4:76512/1‑149 (MQ=255)                                  gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                >  6:73229/1‑149 (MQ=255)                                   cTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggc                                                                                                               <  4:3002/149‑1 (MQ=255)                                    tAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggcg                                                                                                              >  8:78064/1‑149 (MQ=255)                                     aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      >  8:276662/1‑140 (MQ=255)                                     aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      <  7:276662/140‑1 (MQ=255)                                      aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            <  8:103657/149‑1 (MQ=255)                                      aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            >  6:10188/1‑149 (MQ=255)                                      aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGGCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            <  2:153135/149‑1 (MQ=255)                                           ccAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTCTGAGTATTCTCATGTTCGGCGGCCCCTc                                                                                                       >  3:79653/1‑149 (MQ=255)                                                cGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTCTGAGTATTCTCATGTTCGGCGGCCCCTCGAAgg                                                                                                  <  4:79653/149‑1 (MQ=255)                                                 gTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGt                                                                                                 <  2:302398/149‑1 (MQ=255)                                                  tAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTg                                                                                                >  6:47682/1‑149 (MQ=255)                                                      tgtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACg                                                                                            >  5:263208/1‑149 (MQ=255)                                                       gtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGc                                                                                           >  8:140866/1‑149 (MQ=255)                                                              tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgggtgg                                                                                    >  3:311622/1‑149 (MQ=255)                                                                 taataaGTGGCGTAGTTCATGAATCTGTGCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTa                                                                                 >  1:212013/1‑149 (MQ=255)                                                                 taataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTa                                                                                 >  3:215436/1‑149 (MQ=255)                                                                      aGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                            <  3:163112/149‑1 (MQ=255)                                                                      aGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                            <  1:189754/149‑1 (MQ=255)                                                                             tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGt                                                                     <  6:246858/149‑1 (MQ=255)                                                                                   aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTgg                                                                   >  4:65764/1‑145 (MQ=255)                                                                                   aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTgg                                                                   <  3:65764/145‑1 (MQ=255)                                                                                   aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTa                                                                >  3:12923/1‑148 (MQ=255)                                                                                         cTGTCCTCAAAGTGAATCGGGGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCaa                                                          >  3:349033/1‑148 (MQ=255)                                                                                           gTCCTCAAAGTGAAGCGTTGTTGGTCACAGGCCGGAAAAACAAACCCCCCGGGCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGGGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAc                                                       <  6:69939/149‑1 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 >  1:85120/1‑149 (MQ=255)                                                                                                 aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 <  5:10188/149‑1 (MQ=255)                                                                                                      gAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCaaa                                                  <  2:84261/143‑1 (MQ=255)                                                                                                      gAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCaaa                                                  >  1:84261/1‑143 (MQ=255)                                                                                                         tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                     >  2:71795/1‑137 (MQ=255)                                                                                                         tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                     <  1:71795/137‑1 (MQ=255)                                                                                                         tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCt                                         >  5:23988/1‑149 (MQ=255)                                                                                                            gtgtTGGTCAAAGGCCTG‑AAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       >  5:96716/1‑147 (MQ=255)                                                                                                             tgtTGGTCAAAGGCCTGAAAAACAAACCCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCa                                     <  5:3561/149‑1 (MQ=255)                                                                                                             tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCTAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATa                                            <  3:69533/142‑1 (MQ=255)                                                                                                             tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATa                                            >  4:69533/1‑142 (MQ=255)                                                                                                             tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCa                                     <  1:63614/149‑1 (MQ=255)                                                                                                               ttGGTCAAAGGCCTG‑AAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGt                                  <  6:96716/149‑1 (MQ=255)                                                                                                                  gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                <  4:349033/149‑1 (MQ=255)                                                                                                                  gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                <  2:87242/149‑1 (MQ=255)                                                                                                                  gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                >  7:225029/1‑149 (MQ=255)                                                                                                                   tCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGcc                               >  3:117957/1‑149 (MQ=255)                                                                                                                         gCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGCCGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAg                         >  7:268892/1‑149 (MQ=255)                                                                                                                           cTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 >  2:220278/1‑123 (MQ=255)                                                                                                                           cTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 <  1:220278/123‑1 (MQ=255)                                                                                                                              aaaaaCAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGGCg                      <  4:311622/147‑1 (MQ=255)                                                                                                                              aaaaaCAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     >  2:91928/1‑148 (MQ=255)                                                                                                                               aaaacaaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       <  4:36350/129‑1 (MQ=255)                                                                                                                               aaaacaaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       >  3:36350/1‑129 (MQ=255)                                                                                                                                   caaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACgg               >  8:2305/1‑149 (MQ=255)                                                                                                                                     aaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGcc             <  4:34415/149‑1 (MQ=255)                                                                                                                                     aaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     <  2:375712/141‑1 (MQ=255)                                                                                                                                     aaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     >  1:375712/1‑141 (MQ=255)                                                                                                                                      aacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCa            <  7:140866/149‑1 (MQ=255)                                                                                                                                        cccccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAg           >  1:206404/1‑148 (MQ=255)                                                                                                                                          cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  3:283478/1‑148 (MQ=255)                                                                                                                                          cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  2:284609/1‑148 (MQ=255)                                                                                                                                           ccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  7:19597/1‑147 (MQ=255)                                                                                                                                             ggTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAgg     <  5:73229/149‑1 (MQ=255)                                                                                                                                                 gggAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGgcg  >  7:117472/1‑148 (MQ=255)                                                                                                                                                  ggAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGgcg  >  3:84478/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ATCGGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCG  >  NC_002947/3465909‑3466200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑