Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,466,058	G→C	10.8%	noncoding (102/119 nt)	PP_mr39 ←	P15 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,466,058	0	G	C	10.8%	207.4 / 13.8	74	noncoding (102/119 nt)	PP_mr39	P15 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base C (6/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

GGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCG  >  NC_002947/3465912‑3466200                                                                                                                                                   |                                                                                                                                               ggAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGc                                                                                                                                               <  7:165884/148‑1 (MQ=255)         cAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCgggg                                                                                                                                      <  7:69374/149‑1 (MQ=255)             cTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTGTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                  <  7:233142/149‑1 (MQ=255)              tGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                  >  4:119716/1‑148 (MQ=255)                 cccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGt                                                                                                                              >  2:189754/1‑149 (MQ=255)                 cccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgtt                                                                                                                              >  1:173831/1‑145 (MQ=39)                  ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgtta                                                                                                                             >  5:31982/1‑144 (MQ=39)                  ccGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGCTTCCCGACCGGGGGttttgt                                                                                                                               <  6:31982/147‑4 (MQ=39)                       cGGGTTTGGCTAAATCGCCAGGCGTTTGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATtctc                                                                                                                        <  2:9271/149‑1 (MQ=255)                        gggATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCa                                                                                                                       >  3:34415/1‑149 (MQ=255)                         ggATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      <  2:107713/149‑1 (MQ=255)                           aTTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGt                                                                                                                    >  2:63614/1‑149 (MQ=255)                               gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAATACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                >  4:76512/1‑149 (MQ=255)                               gCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcgg                                                                                                                >  6:73229/1‑149 (MQ=255)                                cTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggc                                                                                                               <  4:3002/149‑1 (MQ=255)                                 tAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggcg                                                                                                              >  8:78064/1‑149 (MQ=255)                                  aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      >  8:276662/1‑140 (MQ=255)                                  aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAt                                                                                                                      <  7:276662/140‑1 (MQ=255)                                   aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            <  8:103657/149‑1 (MQ=255)                                   aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            >  6:10188/1‑149 (MQ=255)                                   aaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGGCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCggcggc                                                                                                            <  2:153135/149‑1 (MQ=255)                                        ccAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTCTGAGTATTCTCATGTTCGGCGGCCCCTc                                                                                                       >  3:79653/1‑149 (MQ=255)                                             cGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTCTGAGTATTCTCATGTTCGGCGGCCCCTCGAAgg                                                                                                  <  4:79653/149‑1 (MQ=255)                                              gTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGt                                                                                                 <  2:302398/149‑1 (MQ=255)                                               tAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTg                                                                                                >  6:47682/1‑149 (MQ=255)                                                   tgtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACg                                                                                            >  5:263208/1‑149 (MQ=255)                                                    gtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGc                                                                                           >  8:140866/1‑149 (MQ=255)                                                           tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCgggtgg                                                                                    >  3:311622/1‑149 (MQ=255)                                                              taataaGTGGCGTAGTTCATGAATCTGTGCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTa                                                                                 >  1:212013/1‑149 (MQ=255)                                                              taataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTa                                                                                 >  3:215436/1‑149 (MQ=255)                                                                   aGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                            <  3:163112/149‑1 (MQ=255)                                                                   aGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                            <  1:189754/149‑1 (MQ=255)                                                                          tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGt                                                                     <  6:246858/149‑1 (MQ=255)                                                                                aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTgg                                                                   <  3:65764/145‑1 (MQ=255)                                                                                aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTgg                                                                   >  4:65764/1‑145 (MQ=255)                                                                                aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTa                                                                >  3:12923/1‑148 (MQ=255)                                                                                      cTGTCCTCAAAGTGAATCGGGGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCaa                                                          >  3:349033/1‑148 (MQ=255)                                                                                        gTCCTCAAAGTGAAGCGTTGTTGGTCACAGGCCGGAAAAACAAACCCCCCGGGCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGGGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAc                                                       <  6:69939/149‑1 (MQ=255)                                                                                              aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 >  1:85120/1‑149 (MQ=255)                                                                                              aaaGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 <  5:10188/149‑1 (MQ=255)                                                                                                   gAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCaaa                                                  <  2:84261/143‑1 (MQ=255)                                                                                                   gAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCaaa                                                  >  1:84261/1‑143 (MQ=255)                                                                                                      tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                     >  2:71795/1‑137 (MQ=255)                                                                                                      tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                     <  1:71795/137‑1 (MQ=255)                                                                                                      tCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCt                                         >  5:23988/1‑149 (MQ=255)                                                                                                         gtgtTGGTCAAAGGCCTG‑AAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       >  5:96716/1‑147 (MQ=255)                                                                                                          tgtTGGTCAAAGGCCTGAAAAACAAACCCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCa                                     <  5:3561/149‑1 (MQ=255)                                                                                                          tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCTAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATa                                            <  3:69533/142‑1 (MQ=255)                                                                                                          tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATa                                            >  4:69533/1‑142 (MQ=255)                                                                                                          tgtTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCa                                     <  1:63614/149‑1 (MQ=255)                                                                                                            ttGGTCAAAGGCCTG‑AAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGt                                  <  6:96716/149‑1 (MQ=255)                                                                                                               gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                <  2:87242/149‑1 (MQ=255)                                                                                                               gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                <  4:349033/149‑1 (MQ=255)                                                                                                               gTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGc                                >  7:225029/1‑149 (MQ=255)                                                                                                                tCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGcc                               >  3:117957/1‑149 (MQ=255)                                                                                                                      gCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGCCGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAg                         >  7:268892/1‑149 (MQ=255)                                                                                                                        cTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 >  2:220278/1‑123 (MQ=255)                                                                                                                        cTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAg                                                 <  1:220278/123‑1 (MQ=255)                                                                                                                           aaaaaCAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGGCg                      <  4:311622/147‑1 (MQ=255)                                                                                                                           aaaaaCAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     >  2:91928/1‑148 (MQ=255)                                                                                                                            aaaacaaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       <  4:36350/129‑1 (MQ=255)                                                                                                                            aaaacaaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTgg                                       >  3:36350/1‑129 (MQ=255)                                                                                                                                caaaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACgg               >  8:2305/1‑149 (MQ=255)                                                                                                                                  aaacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGcc             <  4:34415/149‑1 (MQ=255)                                                                                                                                  aaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     >  1:375712/1‑141 (MQ=255)                                                                                                                                  aaacCCCCGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCgg                     <  2:375712/141‑1 (MQ=255)                                                                                                                                   aacCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCa            <  7:140866/149‑1 (MQ=255)                                                                                                                                     cccccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAg           >  1:206404/1‑148 (MQ=255)                                                                                                                                       cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  2:284609/1‑148 (MQ=255)                                                                                                                                       cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  3:283478/1‑148 (MQ=255)                                                                                                                                        ccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca         >  7:19597/1‑147 (MQ=255)                                                                                                                                          ggTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAgg     <  5:73229/149‑1 (MQ=255)                                                                                                                                              gggAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGgcg  >  7:117472/1‑148 (MQ=255)                                                                                                                                               ggAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGgcg  >  3:84478/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                               GGAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCG  >  NC_002947/3465912‑3466200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑