Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,536,808	A→T	11.5%	D149V (GAC→GTC)	PP_3125 →	Cro/CI family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,536,808	0	A	T	11.5%	159.5 / 10.9	61	D149V (GAC→GTC)	PP_3125	Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/28);  new base T (4/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

GCTGCCGTTTCTGATTCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGTGAAA  >  NC_002947/3536668‑3536952                                                                                                                                             |                                                                                                                                                  gcggcCGTTTCTGATTCACCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGGGGATTTCATGAGGGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGc                                                                                                                                           <  8:247667/146‑1 (MQ=255)     ccGTTTCTGATTCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGTCGCCCTGctcca                                                                                                                                       <  7:192100/149‑5 (MQ=255)       gTTTCTGATTCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGTCGCCCTGctccagc                                                                                                                                     <  6:213862/149‑7 (MQ=255)          tCTGATTCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑Ac                                                                                                                                  <  2:279546/148‑1 (MQ=255)                tCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGa                                                                                                                           >  2:198582/1‑149 (MQ=255)                  aGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGaca                                                                                                                         <  8:149678/149‑1 (MQ=255)                   gccgccGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGacac                                                                                                                        >  6:135567/1‑149 (MQ=255)                     cgccgTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGAcaccg                                                                                                                      <  2:239242/149‑1 (MQ=255)                      gccgTCCCAGTTCAGCGACCCTACGTTCAACGAACACCAGGCCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTCGATTTCATGAGTGAGCGGGTCTTGCTGGAGCAGGGCGACGCCCTGCAATTCA‑ACGCCCAGAcaccg                                                                                                                      >  4:176646/1‑148 (MQ=255)                             gAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCt                                                                                                              <  2:343247/149‑1 (MQ=255)                                ttCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTgcg                                                                                                           >  2:53167/1‑149 (MQ=255)                                ttCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTgcg                                                                                                           >  6:4588/1‑149 (MQ=255)                                    gCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCg                                                                                                       <  4:147674/149‑1 (MQ=255)                                     cGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCgg                                                                                                      <  1:53167/149‑1 (MQ=255)                                      gACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCgg                                                                                                      >  6:367534/1‑148 (MQ=255)                                         ccTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAgg                                                                                                  >  2:111411/1‑149 (MQ=255)                                          ctccgctCACGGACCACCCGGTCGAAGAGTTCCTGTTTGATCACACAGGCCAGGTGGCAGGGGATTTCATGAGTGAGCGGGTGTTTCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGgt                                                                                                 <  4:300915/143‑1 (MQ=255)                                          cTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGgt                                                                                                 <  5:367534/149‑1 (MQ=255)                                              gTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGa                                                                                             >  4:205456/1‑149 (MQ=255)                                                tCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACg                                                                                           >  3:65211/1‑149 (MQ=255)                                                tCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACg                                                                                           >  1:348707/1‑149 (MQ=255)                                                  aaGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGcc                                                                                         >  7:204321/1‑149 (MQ=255)                                                  aaGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGc                                                                                          <  2:351874/148‑1 (MQ=255)                                                      aaCACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGgcg                                                                                     >  2:100526/1‑149 (MQ=255)                                                      aaCACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGgcg                                                                                     <  1:100526/149‑1 (MQ=255)                                                             gggCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTAc                                                                              <  3:92910/149‑1 (MQ=255)                                                             gggCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTAc                                                                              >  8:606/1‑149 (MQ=255)                                                                 gAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGgt                                                                          >  7:287131/1‑149 (MQ=255)                                                                   agagTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGgttg                                                                        <  5:135567/149‑1 (MQ=255)                                                                       ttCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGgttgttgt                                                                    >  4:210393/1‑149 (MQ=255)                                                                         ccTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGgttgttgtt                                                                   <  7:233711/148‑1 (MQ=255)                                                                          cTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCATCCGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTc                                                                  <  1:111411/149‑1 (MQ=255)                                                                              ttgttCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTcaccac                                                             >  1:55078/1‑149 (MQ=255)                                                                                      acaGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACg                                                      >  7:22900/1‑148 (MQ=255)                                                                                      acaGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGa                                                     <  3:130029/149‑1 (MQ=255)                                                                                                    aGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAgg                                       <  3:205456/149‑1 (MQ=255)                                                                                                     gTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGATCGGTAGGTGTGCCGCCGGCGCAGTTACTGGTTGTGTTTCCCCACGCAGCCGAATGAAAGCGCAAggg                                      >  4:43664/1‑149 (MQ=255)                                                                                                      tGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGAc                                                       <  5:26063/131‑1 (MQ=255)                                                                                                      tGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGAc                                                       >  6:26063/1‑131 (MQ=255)                                                                                                      tGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGAc                                                       >  2:131838/1‑131 (MQ=255)                                                                                                      tGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGAc                                                       <  1:131838/131‑1 (MQ=255)                                                                                                        gATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGc                                   <  8:287131/149‑1 (MQ=255)                                                                                                              aTGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTAt                             >  2:320483/1‑149 (MQ=255)                                                                                                                 agtgagCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGc                           <  8:204321/148‑1 (MQ=255)                                                                                                                  gtgagCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGccc                         >  2:334918/1‑149 (MQ=255)                                                                                                                         ggTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCacac                  <  3:210393/149‑1 (MQ=255)                                                                                                                         ggTGTTGCTGGAGCAGGGCGAAGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCacac                  >  1:114961/1‑149 (MQ=255)                                                                                                                             ttGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGtt               <  2:348707/148‑1 (MQ=255)                                                                                                                              ttgaaatgCAGGGCGTCGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCCCACGTtc              >  4:210712/8‑148 (MQ=255)                                                                                                                               gCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTtct             >  3:335897/1‑148 (MQ=255)                                                                                                                               gCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTtct             <  5:4588/148‑1 (MQ=255)                                                                                                                                   gAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAgg         <  1:224190/148‑1 (MQ=255)                                                                                                                                   atgCAGGGCGTCGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAgggg                                     >  7:187483/3‑120 (MQ=255)                                                                                                                                   atgCAGGGCGTCGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAgggg                                     <  8:187483/118‑1 (MQ=255)                                                                                                                                   atgCAGGGCGTCGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGc        >  7:188599/3‑149 (MQ=255)                                                                                                                                     gCAGGGCGTCGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGt      >  4:235552/1‑149 (MQ=255)                                                                                                                                     gCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGt      >  7:295240/1‑149 (MQ=255)                                                                                                                                        gggCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGt      >  7:170518/1‑146 (MQ=255)                                                                                                                                        gggCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGTGaa   <  3:43664/149‑1 (MQ=255)                                                                                                                                        gggCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCGCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGt      <  8:170518/146‑1 (MQ=255)                                                                                                                                         ggCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGTGaaa  <  1:334918/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  GCTGCCGTTTCTGATTCAGCCGCCGTCCGAGTTCAGCGACCCTACGTTCAAGGAACACCAGGGCGAAGAGTTCCTGTTTGTTCACACAGGCCAGGTGGAAGTGGATTTCATGAGTGAGCGGGTGTTGCTGGAGCAGGGCGACGCCCTGCATTTCA‑ACGCCCAGACACCGCACCGGCTGCGCTCGGTAGGTGTGACGCCGGCGCAGTTACTGGTTGTTGTTCACCACGCAGACGAATGAAAGCGCAAGGGGGCAACTATGCCCCCCACACGTTCTCAGGCGTGAAA  >  NC_002947/3536668‑3536952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑