Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,614,723	C→A	9.7%	T199N (ACC→AAC) ‡	pet →	protein Pet18

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,614,723	0	C	A	9.7%	176.5 / 7.6	59	T199N (ACC→AAC) ‡	pet	protein Pet18
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base A (2/4);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

AAACCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACG  >  NC_002947/3614575‑3614870                                                                                                                                                     |                                                                                                                                                    aaaCCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCAc                                                                                                                                                     <  1:260523/149‑1 (MQ=255)          ccGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCt                                                                                                                                            <  4:29803/149‑1 (MQ=255)            ggACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTgg                                                                                                                                          <  7:91057/149‑1 (MQ=255)            ggACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTg                                                                                                                                           >  4:188375/1‑148 (MQ=255)             gACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggt                                                                                                                                         <  2:165525/149‑2 (MQ=255)             gACAACTTCGTCCATGCCGACTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTATCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggt                                                                                                                                         <  3:126609/149‑2 (MQ=255)              aCAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggta                                                                                                                                        <  7:68826/149‑3 (MQ=255)                  cTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTgg                                                                                                                                    >  1:208959/1‑149 (MQ=255)                      gTCCATGCCGAGTGGATTACCCTGGATGACAACGCGGCATTGCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCttt                                                                                                                                <  7:203991/149‑1 (MQ=255)                         cATGCCGAGTGGATTACCCTGCATGACACCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTttctt                                                                                                                             >  3:48965/1‑149 (MQ=255)                             ccGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGAt                                                                                                                         <  7:248597/149‑1 (MQ=255)                               gAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGc                                                                                                                       >  6:112916/1‑149 (MQ=255)                                          ccTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCg                                                                                                             >  7:246867/1‑148 (MQ=255)                                          ccTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTACAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCg                                                                                                             >  1:260144/1‑148 (MQ=255)                                           cTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGa                                                                                                           <  2:22125/149‑1 (MQ=255)                                            tGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAg                                                                                                          >  4:211185/1‑149 (MQ=255)                                             gCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAgg                                                                                                         >  5:196502/1‑149 (MQ=255)                                              cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAggg                                                                                                        <  2:140191/149‑1 (MQ=255)                                              cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAgg                                                                                                         <  6:138548/148‑1 (MQ=255)                                              cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAg                                                                                                          <  6:278267/147‑1 (MQ=255)                                                 gACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCa                                                                                                      >  5:817/1‑148 (MQ=255)                                                  aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaaa                                                                                                    <  5:112916/149‑1 (MQ=255)                                                  aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaaa                                                                                                    >  1:7275/1‑149 (MQ=255)                                                  aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaa                                                                                                     <  2:208959/148‑1 (MQ=255)                                                    aaCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTg                                                                                                                                           >  2:338707/1‑108 (MQ=255)                                                    aaCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTg                                                                                                                                           <  1:338707/108‑1 (MQ=255)                                                           cATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATgg                                                                                           <  8:246867/149‑1 (MQ=255)                                                             ttCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGat                                                                                         <  3:211185/149‑1 (MQ=255)                                                                cAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATc                                                                                      >  1:271/1‑149 (MQ=255)                                                                     aTTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTc                                                                                   >  4:141918/1‑147 (MQ=255)                                                                     aTTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTc                                                                                   >  3:60868/1‑147 (MQ=255)                                                                                tCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                            <  3:167367/143‑1 (MQ=255)                                                                                tCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                            >  4:167367/1‑143 (MQ=255)                                                                                  cTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGc                                                                     <  6:196502/148‑1 (MQ=255)                                                                                          cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                            >  6:48089/1‑149 (MQ=255)                                                                                          cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                            <  6:817/149‑1 (MQ=255)                                                                                          cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCAGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                            >  2:360042/1‑149 (MQ=255)                                                                                                  aCCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAAt                                                    <  3:188375/149‑1 (MQ=255)                                                                                                      gttCGGCCTGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGaa                                                >  8:34076/3‑149 (MQ=255)                                                                                                      cgTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGaa                                                <  1:360042/149‑1 (MQ=255)                                                                                                           gCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgc                                                               <  6:334111/129‑1 (MQ=255)                                                                                                           gCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgc                                                               >  5:334111/1‑129 (MQ=255)                                                                                                             ccGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTAc                                         >  6:84263/1‑149 (MQ=255)                                                                                                             ccGCAGGAGGCCGCTTTGGCAAGTGACTTCTACAAGCGCACCGTGGCGCTGGAGCAGGCTTTCTTCGATGCAGTGAACCCGCAGGGCAAATGAGCATGTATATCATCGACCGCCTCAAGGCCGCTGCCACGCCACAATCGAACCGCTAc                                         >  4:269417/1‑149 (MQ=255)                                                                                                              cGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACg                                        <  4:48965/149‑1 (MQ=255)                                                                                                                   gaggCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGAc                                   >  6:102993/1‑149 (MQ=255)                                                                                                                             tGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCg                         >  4:201904/1‑149 (MQ=255)                                                                                                                                cAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGc                      <  2:271/149‑1 (MQ=255)                                                                                                                                   gTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                    >  3:173826/1‑148 (MQ=255)                                                                                                                                      aCTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                    >  6:289046/1‑145 (MQ=255)                                                                                                                                       cTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAAAGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGAt               <  2:7275/149‑1 (MQ=255)                                                                                                                                          gctccAGCGCCACGGTGCGCTGGTGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATggg            >  4:302484/4‑149 (MQ=255)                                                                                                                                            aCCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGgcg          >  1:53502/1‑149 (MQ=255)                                                                                                                                              cAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCATCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                    <  7:42350/137‑1 (MQ=255)                                                                                                                                              cAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCATCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                    >  8:42350/1‑137 (MQ=255)                                                                                                                                               aGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAg       >  4:124953/1‑149 (MQ=255)                                                                                                                                                gcgcACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGa                                    <  6:168755/119‑1 (MQ=255)                                                                                                                                                gcgcACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGa                                    >  5:168755/1‑119 (MQ=255)                                                                                                                                                    aCCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACg  >  5:278941/1‑149 (MQ=255)                                                                                                                                                    aCCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACg  <  3:141918/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AAACCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACG  >  NC_002947/3614575‑3614870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑