Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,614,727	G→T	9.6%	V200V (GTG→GTT) ‡	pet →	protein Pet18

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,614,727	0	G	T	9.6%	179.9 / 6.2	60	V200V (GTG→GTT) ‡	pet	protein Pet18
Reads supporting (aligned to +/- strand):  ref base G (30/24);  new base T (2/4);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACGCT  >  NC_002947/3614584‑3614872                                                                                                                                                |                                                                                                                                                  ccGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCt                                                                                                                                              <  4:29803/149‑1 (MQ=255)   ggACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTgg                                                                                                                                            <  7:91057/149‑1 (MQ=255)   ggACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTg                                                                                                                                             >  4:188375/1‑148 (MQ=255)    gACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggt                                                                                                                                           <  2:165525/149‑2 (MQ=255)    gACAACTTCGTCCATGCCGACTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTATCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggt                                                                                                                                           <  3:126609/149‑2 (MQ=255)     aCAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTggta                                                                                                                                          <  7:68826/149‑3 (MQ=255)         cTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTgg                                                                                                                                      >  1:208959/1‑149 (MQ=255)             gTCCATGCCGAGTGGATTACCCTGGATGACAACGCGGCATTGCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCttt                                                                                                                                  <  7:203991/149‑1 (MQ=255)                cATGCCGAGTGGATTACCCTGCATGACACCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTttctt                                                                                                                               >  3:48965/1‑149 (MQ=255)                    ccGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGAt                                                                                                                           <  7:248597/149‑1 (MQ=255)                      gAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGc                                                                                                                         >  6:112916/1‑149 (MQ=255)                                 ccTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCg                                                                                                               >  7:246867/1‑148 (MQ=255)                                 ccTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTACAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCg                                                                                                               >  1:260144/1‑148 (MQ=255)                                  cTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGa                                                                                                             <  2:22125/149‑1 (MQ=255)                                   tGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAg                                                                                                            >  4:211185/1‑149 (MQ=255)                                    gCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAgg                                                                                                           >  5:196502/1‑149 (MQ=255)                                     cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAggg                                                                                                          <  2:140191/149‑1 (MQ=255)                                     cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAgg                                                                                                           <  6:138548/148‑1 (MQ=255)                                     cATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAg                                                                                                            <  6:278267/147‑1 (MQ=255)                                        gACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCa                                                                                                        >  5:817/1‑148 (MQ=255)                                         aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaaa                                                                                                      >  1:7275/1‑149 (MQ=255)                                         aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaaa                                                                                                      <  5:112916/149‑1 (MQ=255)                                         aCAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCaa                                                                                                       <  2:208959/148‑1 (MQ=255)                                           aaCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTg                                                                                                                                             >  2:338707/1‑108 (MQ=255)                                           aaCCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCCACGGTGCGCTg                                                                                                                                             <  1:338707/108‑1 (MQ=255)                                                  cATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATgg                                                                                             <  8:246867/149‑1 (MQ=255)                                                    ttCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGat                                                                                           <  3:211185/149‑1 (MQ=255)                                                       cAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATc                                                                                        >  1:271/1‑149 (MQ=255)                                                            aTTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTc                                                                                     >  3:60868/1‑147 (MQ=255)                                                            aTTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTc                                                                                     >  4:141918/1‑147 (MQ=255)                                                                       tCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                              <  3:167367/143‑1 (MQ=255)                                                                       tCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                              >  4:167367/1‑143 (MQ=255)                                                                         cTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGc                                                                       <  6:196502/148‑1 (MQ=255)                                                                                 cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                              <  6:817/149‑1 (MQ=255)                                                                                 cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                              >  6:48089/1‑149 (MQ=255)                                                                                 cGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCAGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccac                                                              >  2:360042/1‑149 (MQ=255)                                                                                         aCCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAAt                                                      <  3:188375/149‑1 (MQ=255)                                                                                             gttCGGCCTGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGaa                                                  >  8:34076/3‑149 (MQ=255)                                                                                             cgTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGaa                                                  <  1:360042/149‑1 (MQ=255)                                                                                                  gCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgc                                                                 <  6:334111/129‑1 (MQ=255)                                                                                                  gCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgc                                                                 >  5:334111/1‑129 (MQ=255)                                                                                                    ccGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTAc                                           >  6:84263/1‑149 (MQ=255)                                                                                                    ccGCAGGAGGCCGCTTTGGCAAGTGACTTCTACAAGCGCACCGTGGCGCTGGAGCAGGCTTTCTTCGATGCAGTGAACCCGCAGGGCAAATGAGCATGTATATCATCGACCGCCTCAAGGCCGCTGCCACGCCACAATCGAACCGCTAc                                           >  4:269417/1‑149 (MQ=255)                                                                                                     cGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACg                                          <  4:48965/149‑1 (MQ=255)                                                                                                          gaggCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGAc                                     >  6:102993/1‑149 (MQ=255)                                                                                                                    tGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCg                           >  4:201904/1‑149 (MQ=255)                                                                                                                       cAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGc                        <  2:271/149‑1 (MQ=255)                                                                                                                          gTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                      >  3:173826/1‑148 (MQ=255)                                                                                                                             aCTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                      >  6:289046/1‑145 (MQ=255)                                                                                                                              cTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAAAGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGAt                 <  2:7275/149‑1 (MQ=255)                                                                                                                                 gctccAGCGCCACGGTGCGCTGGTGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATggg              >  4:302484/4‑149 (MQ=255)                                                                                                                                   aCCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGgcg            >  1:53502/1‑149 (MQ=255)                                                                                                                                     cAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCATCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                      <  7:42350/137‑1 (MQ=255)                                                                                                                                     cAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCATCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg                      >  8:42350/1‑137 (MQ=255)                                                                                                                                      aGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAg         >  4:124953/1‑149 (MQ=255)                                                                                                                                       gcgcACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGa                                      >  5:168755/1‑119 (MQ=255)                                                                                                                                       gcgcACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGa                                      <  6:168755/119‑1 (MQ=255)                                                                                                                                           aCCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACg    >  5:278941/1‑149 (MQ=255)                                                                                                                                           aCCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACg    <  3:141918/149‑1 (MQ=255)                                                                                                                                             cGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACGCt  >  1:113049/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                  CCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGTCGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTGGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGGCTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAGGCACGCT  >  NC_002947/3614584‑3614872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑