Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,615,456 A→G 14.9% intergenic (+19/‑210) PP_3186 → / → codB transcriptional regulator/cytosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,615,4560AG14.9% 86.4 / 6.7 40intergenic (+19/‑210)PP_3186/codBtranscriptional regulator/cytosine transporter
Reads supporting (aligned to +/- strand):  ref base A (14/20);  new base G (3/3);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTGGCGGCGCA  >  NC_002947/3615309‑3615604
                                                                                                                                                   |                                                                                                                                                    
aaGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCGTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGAt                                                                                                                                                     <  7:220970/149‑1 (MQ=255)
 aGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGAtt                                                                                                                                                    <  6:358838/149‑1 (MQ=255)
             aaCTGGCCGCTCGGAGCATGACCGAGCAGCGGTTCGCCGTACTGGCATGTATTTTTGGACAGGCGTCCATCGTTGAGGCTGATTTCTGTCTTATGGGGTTGGACGGGACCGTGTAATCCGCCGTGGGTTGGGTAGTAATCAcccagcc                                                                                                                                         <  6:357802/148‑1 (MQ=255)
             aaCTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTAGTAATCAcccagcc                                                                                                                                         <  2:99560/148‑1 (MQ=255)
               cTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTAcccagcccag                                                                                                                                      >  2:64950/1‑149 (MQ=255)
                         gcagcaTGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTg                                                                                                                            <  1:340313/149‑1 (MQ=255)
                         gcagcaTGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTg                                                                                                                            >  3:252441/1‑149 (MQ=255)
                          cagcaTGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTgg                                                                                                                           <  4:327749/149‑1 (MQ=255)
                          cagcaTGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTgg                                                                                                                           <  4:252478/149‑1 (MQ=255)
                           agcaTGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGt                                                                                                                          <  7:73136/149‑1 (MQ=255)
                                   cGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCCTGGGCTGGGTAGTAATCAcccagcccacgg                                                                                                                                    <  4:81574/131‑4 (MQ=38)
                                   cGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCCTGGGCTGGGTAGTAATCAcccagcccacgg                                                                                                                                    >  3:81574/1‑128 (MQ=38)
                                     agcagcGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGgcgc                                                                                                                <  6:308279/149‑1 (MQ=255)
                                                gCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGGTGTTACTACCCACCCCAGGACCGCTTTGGCGCCTTGGGGCGTCAAAATAAg                                                                                                     >  5:196744/1‑149 (MQ=255)
                                                 ccGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAgt                                                                                                    >  7:224535/1‑149 (MQ=255)
                                                      cTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCGGGGGGGTTACTACCCCGCCCCGGCCCGCGTTGGTGGGGGGGCGGCGCACAATAAGTCGGcc                                                                                               >  6:133701/1‑149 (MQ=255)
                                                         gCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCCGGAACGCTTTGGTTCATGGGCGCCGCAAAATAAGTAGTCCCg                                                                                             >  2:208396/1‑148 (MQ=255)
                                                            gggATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGcaca                                                                                         <  4:252441/149‑1 (MQ=255)
                                                                      gCCAGGCCTCCAGCGTGGAGGCGGATTTCTTGCAAATGGGGTTGGAGGGGTCCGTTTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGCGCATGTGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATc                                                                                <  6:196744/148‑1 (MQ=255)
                                                                       ccAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGt                                                                              >  2:250198/1‑149 (MQ=255)
                                                                       ccAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGt                                                                              <  8:224535/149‑1 (MQ=255)
                                                                        cAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCATTGGTGCATGGGCGCCTCAAAATAAATCGTCCCCCACACGCCAGATCGt                                                                              >  8:30922/1‑148 (MQ=255)
                                                                           gCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGAAGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTAATACCCCGCCCAGGACCCCTTTTGTGCATGGGCGGCTCAAAATAAGTCGTGCCGCACAAGCCAGATAGt                                                                              >  6:332622/1‑145 (MQ=255)
                                                                            ccTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGAt                                                                         <  5:275039/149‑1 (MQ=255)
                                                                             cTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATc                                                                        <  1:250198/149‑1 (MQ=255)
                                                                              tCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTTATAACCAGCCCAGGACCGCGTTTGTGCATGGGCGGCGCAAAAAAAGTAGTCCCCCCCCCACCAGATCGTCAGAtag                                                                       >  3:157568/1‑147 (MQ=255)
                                                                              tCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCg                                                                       >  7:348770/1‑149 (MQ=255)
                                                                              tCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTAATACCCACCCCACGACCCCGGTTGTGCATGGGCGCCGCAAAAAAAATAGTCCCCCACACACCAGATAGTCacaaag                                                                       >  2:105268/1‑144 (MQ=255)
                                                                                            ggATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAgtc                                                                                                   <  3:205555/107‑1 (MQ=255)
                                                                                                                 ggACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTg                                     <  7:3459/148‑1 (MQ=255)
                                                                                                                  gACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTgg                                    <  1:105268/148‑1 (MQ=255)
                                                                                                                              tAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCg                        <  1:208396/148‑1 (MQ=255)
                                                                                                                                 aCCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGAttt                    <  1:64950/149‑1 (MQ=255)
                                                                                                                                          ggCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGt           <  5:43800/149‑1 (MQ=255)
                                                                                                                                              gggTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTggcg       >  3:313430/1‑149 (MQ=255)
                                                                                                                                               ggTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTggcgg      >  2:346781/1‑149 (MQ=255)
                                                                                                                                                gTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGca                                                        <  7:165053/98‑1 (MQ=255)
                                                                                                                                                gTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGca                                                        >  8:165053/1‑98 (MQ=255)
                                                                                                                                                gTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGAGTTCTTCCGGGTggcggc     >  6:228577/1‑149 (MQ=255)
                                                                                                                                                 tGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTGgcggcg    <  1:313628/149‑1 (MQ=255)
                                                                                                                                                   aTTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTGGCGGCGCa  <  5:133701/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCCGATTTCTTCCGGGTGGCGGCGCA  >  NC_002947/3615309‑3615604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑