Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,625,537	T→A	6.3%	intergenic (‑50/+27)	PP_3195 ← / ← PP_3196	hypothetical protein/nodulation protein d1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,625,537	0	T	A	6.3%	177.0 / 3.1	64	intergenic (‑50/+27)	PP_3195/PP_3196	hypothetical protein/nodulation protein d1
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base A (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

TCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATG  >  NC_002947/3625391‑3625681                                                                                                                                                          |                                                                                                                                                 tCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTg                                                                                                                                                 <  4:30249/148‑1 (MQ=255) tCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTg                                                                                                                                                 <  7:98997/148‑1 (MQ=255) tCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTg                                                                                                                                                 <  7:66824/148‑1 (MQ=255) tCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGc                                                                                                                                                <  3:86140/149‑1 (MQ=255) tCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGc                                                                                                                                                >  5:171253/1‑149 (MQ=255)    ttCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGgt                                                                                                                                             >  6:175014/1‑149 (MQ=255)          ggTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CGCCAGACACCGCAGCGgtgctgg                                                                                                                                        <  4:310353/149‑5 (MQ=255)          ggTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTgg                                                                                                                                       <  6:96286/149‑1 (MQ=255)          ggTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTgg                                                                                                                                       >  7:345963/1‑149 (MQ=255)           gTCGTGTAGTCCGGCGGCCACTTGAGAGACAGGCTGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGTGTGTTTCCAATCGTGATCA‑‑GGGATGCGAGGTATAGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTgtg                                                                                                                                      <  8:302206/149‑3 (MQ=255)           gTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCTGGGAGGGCAGGGTGTGGCCAATCGTGTTCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑GACCGCTGCGGTGTCTggg                                                                                                                                      <  5:245914/149‑1 (MQ=255)              gTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCgg                                                                                                                                              <  1:341187/138‑1 (MQ=255)              gTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCgg                                                                                                                                              >  2:341187/1‑138 (MQ=255)               tGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGa                                                                                                                                  <  2:194009/149‑1 (MQ=255)                   gTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgc                                                                                                                              >  5:89647/1‑149 (MQ=255)                   cgccGGCGCCCACCTGATCGCAATGCACGCAGGGCGCGGTTTGCATGCAATGGACCCATGGATGGCAGGGGTGGGCCAATCGTGCTCACCTGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATg                                                                                                                                <  1:294317/147‑1 (MQ=255)                      cGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgccgc                                                                                                                           >  1:104152/1‑149 (MQ=255)                      cGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgccgc                                                                                                                           >  5:177543/1‑149 (MQ=255)                       ggCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgccgcc                                                                                                                          >  4:186308/1‑149 (MQ=255)                           cccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTc                                                                                                                      <  6:177543/149‑1 (MQ=255)                            ccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCt                                                                                                                       >  2:166157/1‑147 (MQ=255)                               cTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGc                                                                                                                  >  6:271232/1‑149 (MQ=255)                                  gAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCgg                                                                                                                <  5:285938/148‑1 (MQ=255)                                     cGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGATCGGGTTAGCAC‑CACCGCGGCGGTGTGTGGGGCGATGGCCCCCCCTCACGCgg                                                                                                                >  1:125605/1‑149 (MQ=255)                                        caggcaggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgcg                                                                                                         >  6:372866/1‑149 (MQ=255)                                        caggcaggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgcg                                                                                                         >  2:190658/1‑149 (MQ=255)                                                caggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCAt                                                                                                 <  1:166157/149‑1 (MQ=255)                                                      gcgGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCt                                                                                           <  2:104152/149‑1 (MQ=255)                                                        ggTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGc                                                                                         >  6:320167/1‑149 (MQ=255)                                                                ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCt                                                                                    <  2:24977/146‑1 (MQ=255)                                                                ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCt                                                                                    >  1:24977/1‑146 (MQ=255)                                                                         cgtaggcaggGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGaa                                                                         <  6:89647/145‑1 (MQ=255)                                                                         cGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAAt                                                                        <  5:271232/149‑1 (MQ=255)                                                                              gcagggcaggGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAt                                                                   >  3:271377/1‑149 (MQ=255)                                                                              gcagggcaggGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAt                                                                   >  4:197499/1‑149 (MQ=255)                                                                               cagggcagggTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATc                                                                  <  5:320167/149‑1 (MQ=255)                                                                                agggcagggTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCg                                                                 <  5:372866/149‑1 (MQ=255)                                                                                     agggTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGAt                                                            <  1:326600/149‑1 (MQ=255)                                                                                             gCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTcgc                                                    >  4:67392/1‑149 (MQ=255)                                                                                             gCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTcgc                                                    >  1:179379/1‑149 (MQ=255)                                                                                                aaTCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGaa                                                 <  2:125605/149‑1 (MQ=255)                                                                                                          a‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCtgtg                                       >  2:23613/1‑149 (MQ=255)                                                                                                                 tGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATgg                                  <  5:175014/149‑1 (MQ=255)                                                                                                                   cGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATgggg                                >  8:330090/1‑149 (MQ=255)                                                                                                                    gAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGt                               >  4:296758/1‑149 (MQ=255)                                                                                                                    gAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGt                               >  1:171212/1‑149 (MQ=255)                                                                                                                      ggTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCg                             <  8:345963/149‑1 (MQ=255)                                                                                                                       gTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGc                            >  6:50798/1‑149 (MQ=255)                                                                                                                        tATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGcc                           <  6:171253/149‑1 (MQ=255)                                                                                                                           cgcgAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATTGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTc                        >  1:280660/1‑149 (MQ=255)                                                                                                                            gcgAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTc                        >  8:282295/1‑148 (MQ=255)                                                                                                                               aaaCGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtg                    >  8:103895/1‑149 (MQ=255)                                                                                                                                aaCGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgc                   >  2:62282/1‑149 (MQ=255)                                                                                                                                    cgAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCg               <  4:271377/149‑1 (MQ=255)                                                                                                                                     gAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCgg              >  3:187940/1‑149 (MQ=255)                                                                                                                                     gAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCgg              >  7:264476/1‑149 (MQ=255)                                                                                                                                      aT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGGCGCCGTCCATGCTGCGGt             <  5:78934/149‑1 (MQ=255)                                                                                                                                              cccagacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCTGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCACTCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGcc          >  6:216918/7‑148 (MQ=255)                                                                                                                                              cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGaa                                                                         <  6:155124/85‑1 (MQ=255)                                                                                                                                              cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGaa                                                                         >  5:155124/1‑85 (MQ=255)                                                                                                                                               ccagacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt             <  3:2228/139‑1 (MQ=255)                                                                                                                                               ccagacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt             >  4:2228/6‑144 (MQ=255)                                                                                                                                                     aCCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGGCGCCGTCCATGCTGCGGTGCCACAGCAt   <  1:23613/149‑1 (MQ=255)                                                                                                                                                      ccGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATg  <  3:197499/149‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                 TCCTTCTGCGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCA‑‑GGGATGCGAGGTATCGCGAAACGCGAT‑‑‑G‑CCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATG  >  NC_002947/3625391‑3625681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑