Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,636,403 A→T 12.8% I111F (ATC→TTC)  PP_3203 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,636,4030AT12.8% 120.1 / 8.6 47I111F (ATC→TTC) PP_3203MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (17/24);  new base T (3/3);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGC  >  NC_002947/3636257‑3636548
                                                                                                                                                  |                                                                                                                                                 
ccTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATc                                                                                                                                                 <  6:188571/149‑1 (MQ=255)
ccTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATc                                                                                                                                                 >  2:199241/1‑149 (MQ=255)
     ttgGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCtt                                                                                                                                            <  4:192555/149‑1 (MQ=255)
       ggCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTg                                                                                                                                           <  5:370206/148‑1 (MQ=255)
                ggCCGGGGGGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCg                                                                                                                                 <  4:112343/149‑1 (MQ=255)
                  ccGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                 <  6:224895/147‑1 (MQ=255)
                  ccGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                 >  5:224895/1‑147 (MQ=255)
                    ggggTGTTGGCAGACCGAGGGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCGGcgc                                                                                                                             <  5:267828/149‑3 (MQ=255)
                                   cGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGcac                                                                                                              >  4:307329/1‑149 (MQ=255)
                                    gAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCtt                                                                                                                                            <  1:282807/118‑1 (MQ=255)
                                    gAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCtt                                                                                                                                            >  2:282807/1‑118 (MQ=255)
                                        ggggCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGc                                                                                                         <  8:312913/149‑1 (MQ=255)
                                            cggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGAt                                                                                                     <  3:10039/149‑1 (MQ=255)
                                             ggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCATGCTGATg                                                                                                    <  4:316736/149‑1 (MQ=255)
                                             ggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATg                                                                                                    <  5:254186/149‑1 (MQ=255)
                                              gcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATgg                                                                                                   <  1:67859/149‑1 (MQ=255)
                                              gcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATgg                                                                                                   <  5:214448/149‑1 (MQ=255)
                                                                        tGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCgg                                                                         >  2:250453/1‑149 (MQ=255)
                                                                         gCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCggg                                                                        >  5:295728/1‑149 (MQ=255)
                                                                               cAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGcgcg                                                                    <  3:146702/147‑1 (MQ=255)
                                                                               cAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCgg                                                                   <  7:2419/148‑1 (MQ=255)
                                                                               cAGCCTGTGCGGTTTGTTGCCGTTGGGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGTCATCATGACCTGTTGCAGCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGcgcg                                                                    <  8:113449/147‑1 (MQ=255)
                                                                                  ccTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGgttgt                                                               >  7:185708/1‑149 (MQ=255)
                                                                                        cGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCtt                                                         >  3:209453/1‑149 (MQ=255)
                                                                                          gTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTCTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTg                                                       <  2:61542/149‑1 (MQ=255)
                                                                                           tttGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATGATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGGTGTTCGCTTTg                                                       >  8:284974/1‑148 (MQ=255)
                                                                                                  ccGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGAtggt                                               >  6:4469/1‑149 (MQ=255)
                                                                                                   cGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGAtggtg                                              >  4:56617/1‑149 (MQ=255)
                                                                                                              gACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCTACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCAtt                                   <  2:184945/149‑1 (MQ=255)
                                                                                                                 tCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGCGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggc                                >  1:278358/1‑149 (MQ=255)
                                                                                                                   gCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggc                                <  4:341555/147‑1 (MQ=255)
                                                                                                                    cTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggc                             <  2:268445/149‑1 (MQ=255)
                                                                                                                       gTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggcggc                          >  4:292765/1‑149 (MQ=255)
                                                                                                                       gTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggcg                            <  7:296023/147‑1 (MQ=255)
                                                                                                                         gCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                        >  1:40614/1‑149 (MQ=255)
                                                                                                                            gATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgttgt                     >  3:142219/1‑149 (MQ=255)
                                                                                                                              ttGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTc                   <  1:250453/149‑1 (MQ=255)
                                                                                                                                 tCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAt                 <  1:199241/148‑1 (MQ=255)
                                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGgcgcc                                                                    >  4:262942/1‑93 (MQ=255)
                                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGgcgcc                                                                    <  3:262942/94‑2 (MQ=255)
                                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgggc             >  5:166877/1‑149 (MQ=255)
                                                                                                                                        tgatgcCGGCTTCGGCCAAGCCGATGCCGGCGTCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                        <  7:350363/130‑1 (MQ=38)
                                                                                                                                        tgatgcCGGCTTCGGCCAAGCCGATGCCGGCGTCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                        >  8:350363/5‑134 (MQ=38)
                                                                                                                                           tgcCGGCTTCGGCCAAGCCGATGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgggcgtggggg      >  1:333995/2‑147 (MQ=255)
                                                                                                                                            gcCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTCCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCgg     <  3:307329/149‑1 (MQ=255)
                                                                                                                                              cGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTTTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTg   >  7:190102/1‑149 (MQ=255)
                                                                                                                                               ggCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTgc  <  8:185708/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCTTGTTGGCGCCGCTGGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGC  >  NC_002947/3636257‑3636548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑