Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,636,417 A→T 13.9% E115D (GAA→GAT)  PP_3203 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,636,4170AT13.9% 107.9 / 8.9 43E115D (GAA→GAT) PP_3203MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base T (3/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGCGCTGGGGG  >  NC_002947/3636273‑3636556
                                                                                                                                                |                                                                                                                                           
ggCCGGGGGGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCg                                                                                                                                         <  4:112343/149‑1 (MQ=255)
  ccGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                         <  6:224895/147‑1 (MQ=255)
  ccGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCg                                                                                                                                         >  5:224895/1‑147 (MQ=255)
    ggggTGTTGGCAGACCGAGGGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCTTCGGCCAAGCCGATGCCGGcgc                                                                                                                                     <  5:267828/149‑3 (MQ=255)
                   cGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGcac                                                                                                                      >  4:307329/1‑149 (MQ=255)
                        ggggCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGc                                                                                                                 <  8:312913/149‑1 (MQ=255)
                            cggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGAt                                                                                                             <  3:10039/149‑1 (MQ=255)
                             ggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCATGCTGATg                                                                                                            <  4:316736/149‑1 (MQ=255)
                             ggcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATg                                                                                                            <  5:254186/149‑1 (MQ=255)
                              gcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATgg                                                                                                           <  5:214448/149‑1 (MQ=255)
                              gcgAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATgg                                                                                                           <  1:67859/149‑1 (MQ=255)
                                                        tGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCgg                                                                                 >  2:250453/1‑149 (MQ=255)
                                                         gCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCggg                                                                                >  5:295728/1‑149 (MQ=255)
                                                               cAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGcgcg                                                                            <  3:146702/147‑1 (MQ=255)
                                                               cAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCgg                                                                           <  7:2419/148‑1 (MQ=255)
                                                               cAGCCTGTGCGGTTTGTTGCCGTTGGGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGTCATCATGACCTGTTGCAGCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGcgcg                                                                            <  8:113449/147‑1 (MQ=255)
                                                                  ccTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGgttgt                                                                       >  7:185708/1‑149 (MQ=255)
                                                                        cGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCtt                                                                 >  3:209453/1‑149 (MQ=255)
                                                                          gTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTCTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTg                                                               <  2:61542/149‑1 (MQ=255)
                                                                           tttGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATGATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGGTGTTCGCTTTg                                                               >  8:284974/1‑148 (MQ=255)
                                                                                  ccGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGAtggt                                                       >  6:4469/1‑149 (MQ=255)
                                                                                   cGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGAtggtg                                                      >  4:56617/1‑149 (MQ=255)
                                                                                              gACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCTACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCAtt                                           <  2:184945/149‑1 (MQ=255)
                                                                                                 tCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGCGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggc                                        >  1:278358/1‑149 (MQ=255)
                                                                                                   gCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggc                                        <  4:341555/147‑1 (MQ=255)
                                                                                                    cTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggc                                     <  2:268445/149‑1 (MQ=255)
                                                                                                       gTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggcggc                                  >  4:292765/1‑149 (MQ=255)
                                                                                                       gTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTggcggcg                                    <  7:296023/147‑1 (MQ=255)
                                                                                                         gCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                                >  1:40614/1‑149 (MQ=255)
                                                                                                            gATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgttgt                             >  3:142219/1‑149 (MQ=255)
                                                                                                              ttGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTc                           <  1:250453/149‑1 (MQ=255)
                                                                                                                 tCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAt                         <  1:199241/148‑1 (MQ=255)
                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGgcgcc                                                                            >  4:262942/1‑93 (MQ=255)
                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGgcgcc                                                                            <  3:262942/94‑2 (MQ=255)
                                                                                                                    ccAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgggc                     >  5:166877/1‑149 (MQ=255)
                                                                                                                        tgatgcCGGCTTCGGCCAAGCCGATGCCGGCGTCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                                <  7:350363/130‑1 (MQ=38)
                                                                                                                        tgatgcCGGCTTCGGCCAAGCCGATGCCGGCGTCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCgt                                >  8:350363/5‑134 (MQ=38)
                                                                                                                           tgcCGGCTTCGGCCAAGCCGATGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCAtgggcgtggggg              >  1:333995/2‑147 (MQ=255)
                                                                                                                            gcCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTCCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCgg             <  3:307329/149‑1 (MQ=255)
                                                                                                                              cGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTTTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTg           >  7:190102/1‑149 (MQ=255)
                                                                                                                               ggCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTgc          <  8:185708/149‑1 (MQ=255)
                                                                                                                                   tCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCAACTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGCGCTg      >  3:276898/1‑149 (MQ=255)
                                                                                                                                        ttGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGCGCTggggg  >  7:246244/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GGCCGGGGTGTTGGCAGACCGAGTGGGGCGGCGAGCACTGCTGCTGGCGAGCATGTTGCTCTACAGCCTGTGCGGTTTGTTGCCGTTGTGGCTGGACTCGCTGGTGCTGATTGTCGCCAGCCGCGCCGGCATCGGCTTGGCCGAAGCCGGCATCATGACCTGTTGCACCACGCTGATGGGTGACTACTTCGACGGCCAGCGCCGGGCGCGGTTGTTCGCTTTGCAGATGGTGGTCACCTCATTGGCGGCGGCGTTGTTCATGGGCGTGGGCGGTGCGCTGGGGG  >  NC_002947/3636273‑3636556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑