Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,761,710 A→C 8.0% H353P (CAC→CCC)  PP_3325 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,761,7100AC 8.0% 177.8 / 6.5 63H353P (CAC→CCC) PP_3325ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base C (3/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGC  >  NC_002947/3761564‑3761849
                                                                                                                                                  |                                                                                                                                           
gAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACg                                                                                                                                           >  3:38245/1‑149 (MQ=255)
          cTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGa                                                                                                                                 <  8:41906/149‑1 (MQ=255)
            ggCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGc                                                                                                                               >  6:17160/1‑149 (MQ=255)
               cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                            <  4:62414/149‑1 (MQ=255)
               cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTgg                                                                                                                            <  8:24651/149‑1 (MQ=38)
                aaCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTggg                                                                                                                           >  8:243966/1‑149 (MQ=255)
                   cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtg                                                                                                                         <  1:70324/148‑1 (MQ=38)
                   cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtg                                                                                                                         >  2:70324/1‑148 (MQ=38)
                     cTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                   <  7:167823/136‑1 (MQ=255)
                     cTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                   >  8:167823/1‑136 (MQ=255)
                        cacCCAGGGCACGCGCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                    <  6:307703/148‑1 (MQ=255)
                           ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                                 >  3:171638/1‑148 (MQ=255)
                             aGGGCACGCTCAAGGGCACCAGCTCCGAGCCCCCGGACCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGCCAACATCACCCAGGCCCAGCTGCACGGGCAGATCGAGCGGGGTGGCTGgcagca                                                                                                               >  1:248811/1‑148 (MQ=255)
                             aGGGCACGCTCAAGGGCAACAGCTCCGAGCCGCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAAGGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                              <  7:333861/149‑1 (MQ=255)
                             aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                              <  4:38245/149‑1 (MQ=255)
                                  aCGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAc                                                                                                         <  4:192781/149‑1 (MQ=255)
                                    gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGccc                                                                                                        >  6:102829/1‑148 (MQ=255)
                                    gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                       >  4:190036/1‑149 (MQ=255)
                                      tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcagca                                                                                                               <  2:31061/139‑1 (MQ=255)
                                      tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGa                                                                                                          >  6:367213/1‑144 (MQ=255)
                                      tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGa                                                                                                          <  5:367213/144‑1 (MQ=255)
                                      tCAAGGGCAACAGCTCCGAGCCCCAGGCCCAGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcagca                                                                                                               >  1:31061/1‑139 (MQ=255)
                                         aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACGTCGATTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTACGAGCGGGGTGGCTGGCAGCATCAGACCCgggtg                                                                                                  >  8:281547/1‑149 (MQ=255)
                                          gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtgg                                                                                                 >  2:161773/1‑149 (MQ=255)
                                            gCAACACCTCCCAGCCCCACGCCCTGGTGAGCACCACCATCACCCGCTTCTATCGCGAACCCGACTTCGAATTGAACTACAAAATTACCCACGCCGATCTGCACTGGCATTTTGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTgggg                                                                                               <  7:234699/149‑1 (MQ=255)
                                                cAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTg                                                                                            <  8:136465/148‑1 (MQ=255)
                                                cAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTg                                                                                            >  7:136465/1‑148 (MQ=255)
                                                     ccGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                      >  5:330404/1‑149 (MQ=255)
                                                      cGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                      >  3:142527/1‑148 (MQ=255)
                                                      cGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                      >  3:75615/1‑148 (MQ=255)
                                                         gCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGaaa                                                                                  <  5:17160/149‑1 (MQ=255)
                                                           cccAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAAc                                                                                <  6:15904/149‑1 (MQ=255)
                                                            ccAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAAc                                                                                >  8:160606/1‑148 (MQ=255)
                                                             cAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGACCGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAACCTa                                                                              >  2:176723/1‑149 (MQ=255)
                                                              aGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTAc                                                                             <  1:161773/149‑1 (MQ=255)
                                                                            accaccATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGaa                                                               <  3:190036/149‑1 (MQ=255)
                                                                            accaccATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGaa                                                               <  4:127486/149‑1 (MQ=255)
                                                                              caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                              >  7:11824/1‑148 (MQ=255)
                                                                                ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                           >  2:176114/1‑149 (MQ=255)
                                                                                         gcTTCTATCGCGAACGCGACTTCGTTTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                    <  1:289976/83‑1 (MQ=255)
                                                                                         gcTTCTATCGCGAACGCGACTTCGATTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                    >  2:289976/1‑83 (MQ=255)
                                                                                                tCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCAc                                             <  4:171638/147‑1 (MQ=255)
                                                                                                             ttCGAATGGAACGACAACATCACCCAGGCAGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATgg                              >  1:160790/1‑149 (MQ=255)
                                                                                                                gAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCt                           >  6:106871/1‑149 (MQ=255)
                                                                                                                gAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAAGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCt                           >  4:125798/1‑149 (MQ=255)
                                                                                                                    ggAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                        >  4:370169/1‑148 (MQ=255)
                                                                                                                      aaCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAACACTACCGCCACAGCCAGAAGTACCCGCATAGCGCCACGTCCGCAGCGTATGGGCTGGatag                     >  6:4886/1‑148 (MQ=255)
                                                                                                                        cGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCt                   <  5:106871/149‑1 (MQ=255)
                                                                                                                          actacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                 <  7:281547/146‑1 (MQ=255)
                                                                                                                          acaacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                 <  7:160606/149‑1 (MQ=255)
                                                                                                                          acaacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                 <  1:176114/149‑1 (MQ=255)
                                                                                                                           caacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                 >  3:209884/1‑148 (MQ=255)
                                                                                                                                tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg           <  3:370169/149‑1 (MQ=255)
                                                                                                                                tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg           >  1:320600/1‑149 (MQ=255)
                                                                                                                                ccACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg           >  5:223020/2‑149 (MQ=38)
                                                                                                                                  aCCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg          >  3:74329/1‑148 (MQ=255)
                                                                                                                                   cccAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGAGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta        >  6:139222/1‑149 (MQ=255)
                                                                                                                                   cccAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTTCCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGt         >  8:314125/1‑148 (MQ=38)
                                                                                                                                    ccAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta        >  8:311161/1‑148 (MQ=255)
                                                                                                                                      aGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc     <  7:311161/149‑1 (MQ=255)
                                                                                                                                         ccGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTTGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTCGATATCTACAACCCGGTATACGGc  <  8:11824/149‑1 (MQ=255)
                                                                                                                                          cGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                        <  3:364169/126‑1 (MQ=255)
                                                                                                                                          cGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                        >  4:364169/1‑126 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGC  >  NC_002947/3761564‑3761849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑