Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,761,723 G→C 8.1% E357D (GAG→GAC) ‡ PP_3325 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,761,7230GC 8.1% 189.9 / 6.3 62E357D (GAG→GAC) ‡PP_3325ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (33/24);  new base C (3/2);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

GGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCAGCCATCAC  >  NC_002947/3761576‑3761869
                                                                                                                                                   |                                                                                                                                                  
ggCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGc                                                                                                                                                   >  6:17160/1‑149 (MQ=255)
   cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                                                <  4:62414/149‑1 (MQ=255)
   cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTgg                                                                                                                                                <  8:24651/149‑1 (MQ=38)
    aaCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTggg                                                                                                                                               >  8:243966/1‑149 (MQ=255)
       cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtg                                                                                                                                             <  1:70324/148‑1 (MQ=38)
       cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtg                                                                                                                                             >  2:70324/1‑148 (MQ=38)
            cacCCAGGGCACGCGCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                                        <  6:307703/148‑1 (MQ=255)
               ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                                                     >  3:171638/1‑148 (MQ=255)
                 aGGGCACGCTCAAGGGCACCAGCTCCGAGCCCCCGGACCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGCCAACATCACCCAGGCCCAGCTGCACGGGCAGATCGAGCGGGGTGGCTGgcagca                                                                                                                                   >  1:248811/1‑148 (MQ=255)
                 aGGGCACGCTCAAGGGCAACAGCTCCGAGCCGCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAAGGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                                                  <  7:333861/149‑1 (MQ=255)
                 aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                                                  <  4:38245/149‑1 (MQ=255)
                      aCGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAc                                                                                                                             <  4:192781/149‑1 (MQ=255)
                        gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGccc                                                                                                                            >  6:102829/1‑148 (MQ=255)
                        gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                                           >  4:190036/1‑149 (MQ=255)
                          tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcagca                                                                                                                                   <  2:31061/139‑1 (MQ=255)
                          tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGa                                                                                                                              <  5:367213/144‑1 (MQ=255)
                          tCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGa                                                                                                                              >  6:367213/1‑144 (MQ=255)
                          tCAAGGGCAACAGCTCCGAGCCCCAGGCCCAGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcagca                                                                                                                                   >  1:31061/1‑139 (MQ=255)
                             aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACGTCGATTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTACGAGCGGGGTGGCTGGCAGCATCAGACCCgggtg                                                                                                                      >  8:281547/1‑149 (MQ=255)
                              gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtgg                                                                                                                     >  2:161773/1‑149 (MQ=255)
                                gCAACACCTCCCAGCCCCACGCCCTGGTGAGCACCACCATCACCCGCTTCTATCGCGAACCCGACTTCGAATTGAACTACAAAATTACCCACGCCGATCTGCACTGGCATTTTGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTgggg                                                                                                                   <  7:234699/149‑1 (MQ=255)
                                    cAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTg                                                                                                                <  8:136465/148‑1 (MQ=255)
                                    cAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTg                                                                                                                >  7:136465/1‑148 (MQ=255)
                                         ccGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                                          >  5:330404/1‑149 (MQ=255)
                                          cGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                                          >  3:142527/1‑148 (MQ=255)
                                          cGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAc                                                                                                          >  3:75615/1‑148 (MQ=255)
                                             gCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGaaa                                                                                                      <  5:17160/149‑1 (MQ=255)
                                               cccAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAAc                                                                                                    <  6:15904/149‑1 (MQ=255)
                                                ccAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAAc                                                                                                    >  8:160606/1‑148 (MQ=255)
                                                 cAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGACCGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAACCTa                                                                                                  >  2:176723/1‑149 (MQ=255)
                                                  aGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTAc                                                                                                 <  1:161773/149‑1 (MQ=255)
                                                                accaccATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGaa                                                                                   <  4:127486/149‑1 (MQ=255)
                                                                accaccATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGaa                                                                                   <  3:190036/149‑1 (MQ=255)
                                                                  caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                                                  >  7:11824/1‑148 (MQ=255)
                                                                    ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                                               >  2:176114/1‑149 (MQ=255)
                                                                             gcTTCTATCGCGAACGCGACTTCGTTTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                                        <  1:289976/83‑1 (MQ=255)
                                                                             gcTTCTATCGCGAACGCGACTTCGATTGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                                        >  2:289976/1‑83 (MQ=255)
                                                                                    tCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCAc                                                                 <  4:171638/147‑1 (MQ=255)
                                                                                                 ttCGAATGGAACGACAACATCACCCAGGCAGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATgg                                                  >  1:160790/1‑149 (MQ=255)
                                                                                                    gAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCt                                               >  6:106871/1‑149 (MQ=255)
                                                                                                    gAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTAAGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCt                                               >  4:125798/1‑149 (MQ=255)
                                                                                                        ggAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                                            >  4:370169/1‑148 (MQ=255)
                                                                                                          aaCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAACACTACCGCCACAGCCAGAAGTACCCGCATAGCGCCACGTCCGCAGCGTATGGGCTGGatag                                         >  6:4886/1‑148 (MQ=255)
                                                                                                            cGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCt                                       <  5:106871/149‑1 (MQ=255)
                                                                                                              actacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                                     <  7:281547/146‑1 (MQ=255)
                                                                                                              acaacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                                     <  1:176114/149‑1 (MQ=255)
                                                                                                              acaacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                                     <  7:160606/149‑1 (MQ=255)
                                                                                                               caacaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTAc                                     >  3:209884/1‑148 (MQ=255)
                                                                                                                    tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg                               <  3:370169/149‑1 (MQ=255)
                                                                                                                    tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg                               >  1:320600/1‑149 (MQ=255)
                                                                                                                    ccACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCg                               >  5:223020/2‑149 (MQ=38)
                                                                                                                      aCCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg                              >  3:74329/1‑148 (MQ=255)
                                                                                                                       cccAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGAGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta                            >  6:139222/1‑149 (MQ=255)
                                                                                                                       cccAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTTCCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGt                             >  8:314125/1‑148 (MQ=38)
                                                                                                                        ccAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta                            >  8:311161/1‑148 (MQ=255)
                                                                                                                          aGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc                         <  7:311161/149‑1 (MQ=255)
                                                                                                                             ccGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTTGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTCGATATCTACAACCCGGTATACGGc                      <  8:11824/149‑1 (MQ=255)
                                                                                                                              cGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                                            <  3:364169/126‑1 (MQ=255)
                                                                                                                              cGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGa                                            >  4:364169/1‑126 (MQ=255)
                                                                                                                                          gCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACACTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAagccag         >  1:261874/1‑149 (MQ=255)
                                                                                                                                              ttCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCAGCCAt     >  7:205173/1‑149 (MQ=255)
                                                                                                                                                 gAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCAGCCATCAc  >  2:350904/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAATGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCAGCCATCAC  >  NC_002947/3761576‑3761869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑