Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,779,129	A→T	10.8%	S112T (TCT→ACT)	nikR ←	nickel‑responsive regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,779,129	0	A	T	10.8%	161.2 / 11.1	65	S112T (TCT→ACT)	nikR	nickel‑responsive regulator
Reads supporting (aligned to +/- strand):  ref base A (28/30);  new base T (4/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

CTGACGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTG  >  NC_002947/3778981‑3779274                                                                                                                                                     |                                                                                                                                                   cTGACGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCgaga                                                                                                                                                    <  1:278317/149‑1 (MQ=255) cTGACGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGGCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCgaga                                                                                                                                                    <  4:93542/149‑1 (MQ=255)  tGACGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCgaga                                                                                                                                                    >  1:358343/1‑148 (MQ=255)      gTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACt                                                                                                                                               >  8:353770/1‑149 (MQ=255)      gTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACt                                                                                                                                               >  5:253367/1‑149 (MQ=255)       ttGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTc                                                                                                                                              >  8:91079/1‑149 (MQ=255)          gACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGc                                                                                                                                            <  3:209596/148‑1 (MQ=255)             ttCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAt                                                                                                                                        >  4:31311/1‑149 (MQ=255)             ttCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAt                                                                                                                                        >  1:13496/1‑149 (MQ=255)               cTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGTTGTCTCGCCAAt                                                                                                                                        <  4:14601/147‑1 (MQ=255)                tGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCg                                                                                                                                     >  6:46182/1‑149 (MQ=255)                   gTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGTTGTCTCGCCAAttgatg                                                                                                                                   <  6:127909/148‑5 (MQ=255)                   gTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAGTCGGcc                                                                                                                                  >  8:44749/1‑149 (MQ=255)                         gCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑Tc                                                                                                                           <  1:365400/149‑1 (MQ=255)                          cccTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGGCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCg                                                                                                                          >  7:197957/1‑149 (MQ=255)                          cccTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCAT‑AATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGc                                                                                                                         >  8:50046/1‑149 (MQ=255)                               gggCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGAGCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGccc                                                                                                                       <  3:19183/147‑1 (MQ=255)                                ggCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTgc                                                                                                                    <  7:113610/149‑1 (MQ=255)                                ggCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTg                                                                                                                     <  3:31311/148‑1 (MQ=255)                                            cACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTgc                                                                                                                    <  6:294972/137‑1 (MQ=255)                                            cACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTgc                                                                                                                    >  5:294972/1‑137 (MQ=255)                                            cACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGAcc                                                                                                        >  1:340483/1‑149 (MQ=255)                                                 aCAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAg                                                                                                   <  2:358343/149‑1 (MQ=255)                                                   aaGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAAATCGACCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGc                                                                                                 >  2:125683/1‑149 (MQ=255)                                                     gTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAg                                                                                               >  1:37761/1‑149 (MQ=255)                                                      tGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGt                                                                                              <  8:197957/149‑1 (MQ=255)                                                        aGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCCCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTg                                                                                             <  4:12739/148‑1 (MQ=255)                                                          gCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGcc                                                                                          >  2:244384/1‑149 (MQ=255)                                                           cAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTCTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGcc                                                                                          <  1:244384/149‑1 (MQ=255)                                                           cAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCg                                                                                         <  2:340483/149‑1 (MQ=255)                                                           cAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACATTGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCg                                                                                         <  6:211550/149‑1 (MQ=255)                                                             aCGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGc                                                                                       <  7:44749/149‑1 (MQ=255)                                                                 cTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATc                                                                                    >  6:167218/1‑148 (MQ=255)                                                                           cTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACAt                                                                         >  2:70889/1‑149 (MQ=255)                                                                                 cTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAggg                                                                   <  7:91079/149‑1 (MQ=255)                                                                                    ccccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGt                                                                  <  5:72828/147‑1 (MQ=255)                                                                                    ccccGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTg                                                                 >  7:4689/1‑148 (MQ=255)                                                                                       cGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGGCGGCATCCAGGTGCACATGCAGGGTGGATc                                                             <  5:46182/149‑1 (MQ=255)                                                                                       cGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAg                                                                                 <  5:229401/129‑1 (MQ=255)                                                                                       cGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAg                                                                                 >  6:229401/1‑129 (MQ=255)                                                                                         gAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCgg                                                           <  2:13496/149‑1 (MQ=255)                                                                                              gggATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCTTCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGt                                                         >  3:319085/1‑146 (MQ=255)                                                                                                    acctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtg                                                <  6:347472/149‑1 (MQ=255)                                                                                                     cctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtgg                                               <  7:353770/149‑1 (MQ=255)                                                                                                     cctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtgg                                               >  8:90699/1‑149 (MQ=255)                                                                                                      ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtggt                                              <  2:37761/149‑1 (MQ=255)                                                                                                      ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtggt                                              >  7:240146/1‑149 (MQ=255)                                                                                                      ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCgtggt                                              <  4:319085/149‑1 (MQ=255)                                                                                                        gcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAgg                                                                    >  2:361928/1‑125 (MQ=255)                                                                                                        gcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAgg                                                                    <  1:361928/125‑1 (MQ=255)                                                                                                        gcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGtggtgg                                            >  4:346975/1‑149 (MQ=255)                                                                                                                ccATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTc                                                        <  7:112552/129‑1 (MQ=255)                                                                                                                ccATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTc                                                        >  8:112552/1‑129 (MQ=255)                                                                                                                 cATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAgg                                   <  8:240146/149‑1 (MQ=255)                                                                                                                 cATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAgg                                   <  7:90699/149‑1 (MQ=255)                                                                                                                   tGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑ACGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGt                                 <  2:274986/149‑1 (MQ=255)                                                                                                                      tCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAgg                                   <  8:352709/144‑1 (MQ=255)                                                                                                                      tCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAgg                                   >  7:352709/1‑144 (MQ=255)                                                                                                                             cgcgCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGc                       >  2:216158/1‑149 (MQ=255)                                                                                                                              gcgcTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGcc                      <  6:253367/149‑1 (MQ=255)                                                                                                                                 cTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTc                   >  6:130304/1‑149 (MQ=255)                                                                                                                                      ggccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGa                         >  3:322806/6‑138 (MQ=255)                                                                                                                                      ggccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGa                         <  4:322806/133‑1 (MQ=255)                                                                                                                                        cataaTTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCGCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAg            <  7:50046/146‑1 (MQ=255)                                                                                                                                          gaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGtt          >  7:349021/2‑149 (MQ=255)                                                                                                                                          gaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGtt          >  3:103701/2‑149 (MQ=255)                                                                                                                                          gaaTTGGCGAGTTGTCTCGCCAATCCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCC‑GGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTc         >  6:3524/2‑149 (MQ=255)                                                                                                                                            aTTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTcg        >  2:34879/1‑149 (MQ=255)                                                                                                                                                  gagaCAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTg  <  5:130304/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTGACGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTT‑TCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTG  >  NC_002947/3778981‑3779274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑