Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,784,244 A→G 6.0% I190V (ATC→GTC)  nikE → nickel ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,784,2440AG 6.0% 196.4 / 2.5 66I190V (ATC→GTC) nikEnickel ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (30/32);  new base G (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

GCGCCGAGCATGCCCAACGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGA  >  NC_002947/3784098‑3784392
                                                                                                                                                  |                                                                                                                                                    
gcgcCGCGCATGCCCAACGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGTCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATc                                                                                                                                                    >  6:120198/1‑149 (MQ=255)
  gcCGAGCATGCCCAACGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCt                                                                                                                                                  >  2:290702/1‑149 (MQ=255)
              cAACGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTgg                                                                                                                                       >  4:276292/1‑148 (MQ=255)
              cAACGCCTGCCGCAGCAGTTGAGTGGCGCCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTgg                                                                                                                                       >  1:213506/1‑148 (MQ=255)
               aaCGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATc                                                                                                                                                    <  5:377796/134‑1 (MQ=255)
               aaCGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATc                                                                                                                                                    >  6:377796/1‑134 (MQ=255)
                aCGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAg                                                                                                                                    <  6:378489/149‑1 (MQ=255)
                  gCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGcc                                                                                                                                  <  8:32827/149‑1 (MQ=255)
                   ccTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGccc                                                                                                                                 >  7:86182/1‑149 (MQ=255)
                    cTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGccc                                                                                                                                 >  5:144064/1‑148 (MQ=255)
                    cTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCt                                                                                                                                <  6:7577/149‑1 (MQ=255)
                           agcagTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCcag                                                                                                                         <  8:150007/149‑1 (MQ=255)
                               gTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCcagcg                                                                                                                       >  5:53900/1‑147 (MQ=255)
                                       gcggcCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCACGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGATGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCACTACGCCAGCGCAGCGGCAcc                                                                                                             >  8:226468/1‑149 (MQ=255)
                                        cggcCTGTTGCAGGGTGCGAAGATTGCCCGGGCGCTGGCCGCCTAGACACAGCTTGTAGTGCTCGACGATGCGGTGTCGAACCTGGACCGGCTGTTGCATCTGCAGATCCTGCAGCAGCTGGATGCCCTACTCCAGCTCTGCGGCACCg                                                                                                            <  4:98547/149‑1 (MQ=255)
                                                  cAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGGAGTGCTCGACGAAGCGTTGTCGCACCTGGACCGGCTGTTGCAGCGGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGc                                                                                                   <  7:260631/148‑1 (MQ=255)
                                                    gCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGa                                                                                                <  3:276292/149‑1 (MQ=255)
                                                       tGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCa                                                                                             <  2:237740/149‑1 (MQ=255)
                                                        gCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAg                                                                                            >  7:210588/1‑149 (MQ=255)
                                                         ccAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGc                                                                                           <  2:246236/149‑1 (MQ=255)
                                                          cAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGc                                                                                           >  4:347811/1‑148 (MQ=255)
                                                           aaCATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGGTCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGa                                                                                                >  8:84009/1‑142 (MQ=255)
                                                           aaCATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGGTCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGa                                                                                                <  7:84009/142‑1 (MQ=255)
                                                           aaCATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCa                                                                                         >  4:132591/1‑149 (MQ=255)
                                                            aCATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCAt                                                                                        >  3:2073/1‑149 (MQ=255)
                                                               ttGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGAc                                                                                     <  1:290702/149‑1 (MQ=255)
                                                               ttGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGAc                                                                                     <  6:99715/149‑1 (MQ=255)
                                                                  cccGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCt                                                                                   <  3:347811/148‑1 (MQ=255)
                                                                  cccGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGCCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCt                                                                                   >  2:114927/1‑148 (MQ=255)
                                                                   ccGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGa                                                                                 <  3:132591/149‑1 (MQ=255)
                                                                        cgcTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTg                                                                            <  8:354284/149‑1 (MQ=255)
                                                                         gcTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTgg                                                                           <  2:213506/149‑1 (MQ=255)
                                                                           tGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTg                                                                         <  8:121857/149‑1 (MQ=255)
                                                                                     gCCACAGCTTGTAGTGCTCGTCGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCGAGCGCAGCGGCACGGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCt                                                               <  7:226468/149‑1 (MQ=255)
                                                                                     gCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGACGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCt                                                               >  5:265883/1‑149 (MQ=255)
                                                                                      ccACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTg                                                              <  8:210588/149‑1 (MQ=255)
                                                                                           gCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGc                                                         >  7:117112/1‑149 (MQ=255)
                                                                                              ggTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCggg                                                      >  6:10937/1‑149 (MQ=255)
                                                                                              ggTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCggg                                                      >  2:82048/1‑149 (MQ=255)
                                                                                               gTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGGCCTGAGCCTGGTGCAg                                                                      >  5:348045/1‑132 (MQ=255)
                                                                                               gTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGGCCTGAGCCTGGTGCAg                                                                      <  6:348045/132‑1 (MQ=255)
                                                                                               gTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCt                                                               >  1:188799/1‑139 (MQ=255)
                                                                                               gTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCt                                                               <  2:188799/139‑1 (MQ=255)
                                                                                               gTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCggg                                                      <  8:86182/148‑1 (MQ=255)
                                                                                                tAGGGCTCGACGAAGCGGTGGCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGtt                                                    <  8:258295/149‑1 (MQ=255)
                                                                                                     cTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGtgc                                                <  5:10937/148‑1 (MQ=255)
                                                                                                         acgaAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGtgctgctg                                           >  7:249946/1‑149 (MQ=255)
                                                                                                         acgaAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGtgctgctg                                           >  1:224430/1‑149 (MQ=255)
                                                                                                          cgaAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGtgctgctgc                                          <  8:117112/149‑1 (MQ=255)
                                                                                                             aGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCAcg                                       <  6:144064/149‑1 (MQ=255)
                                                                                                              gCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCAcgg                                      >  4:355842/1‑149 (MQ=255)
                                                                                                                    tCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACggcgggcg                                >  1:219505/1‑149 (MQ=255)
                                                                                                                    tCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACggcgggc                                 <  3:360337/148‑1 (MQ=255)
                                                                                                                    tCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACggcgggc                                 <  5:120198/148‑1 (MQ=255)
                                                                                                                        aCCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCggg                                                      >  6:194006/1‑123 (MQ=255)
                                                                                                                        aCCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCggg                                                      <  5:194006/123‑1 (MQ=255)
                                                                                                                          cTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGt                          >  7:115246/1‑149 (MQ=255)
                                                                                                                           tGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTg                         >  8:52585/1‑149 (MQ=255)
                                                                                                                            ggACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTg                         >  3:245823/1‑148 (MQ=255)
                                                                                                                             gACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTgga                       <  4:245823/149‑1 (MQ=255)
                                                                                                                             gACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTgg                        <  6:53900/148‑1 (MQ=255)
                                                                                                                                      ttGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGccc              >  8:354700/1‑149 (MQ=255)
                                                                                                                                         ctgCTGCAGGATCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGc                <  5:335850/142‑1 (MQ=255)
                                                                                                                                         ctgCTGCAGGATCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGc                >  6:335850/3‑144 (MQ=255)
                                                                                                                                          aGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGt           >  3:370706/1‑148 (MQ=255)
                                                                                                                                            cTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTcac        <  2:224430/149‑1 (MQ=255)
                                                                                                                                                  aTCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGa  >  6:378593/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCGCCGAGCATGCCCAACGCCTGCCGCAGCAGTTGAGTGGCGGCCAGTTGCAGCGTGCCAACATTGCCCGGGCGCTGGCCGCCTCGCCACAGCTGGTAGTGCTCGACGAAGCGTTGTCGAACCTGGACCGGCTGTTGCAGCTGCAGATCCTGCAGCAGCTGGAAGCCCTACGCCAGCGCAGCGGCACCGCTTTCGTGCTGATCAGCCATGACCTGAGCCTGGTGCAGTACTTCTGCCAGCGGGTTGTGCTGCTGCACGGCGGGCGCATCGTGGAGGAACGCCCCGTCACGCACGA  >  NC_002947/3784098‑3784392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑