Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 317,711 C→G 7.2% E74Q (GAA→CAA)  dctD‑I ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947317,7110CG 7.2% 216.1 / 4.0 78E74Q (GAA→CAA) dctD‑IC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (36/36);  new base G (4/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAGGCT  >  NC_002947/317572‑317859
                                                                                                                                           |                                                                                                                                                      
tCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAAc                                                                                                                                                 <  1:90275/147‑1 (MQ=255)
   aGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGccg                                                                                                                                            >  6:282801/1‑147 (MQ=255)
   aGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCC‑TGCAGTTGCTGGAACAACTGCacg                                                                                                                                           <  4:235505/149‑3 (MQ=38)
        ggCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCAt                                                                                                                                      <  3:364588/149‑1 (MQ=255)
                tgGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCTTCGCCTGCACGGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCTGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCTACTGCA‑GGCCATCGATG‑c                                                                                                                               <  2:75482/147‑1 (MQ=255)
                tgGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑ccc                                                                                                                             <  6:147367/149‑1 (MQ=255)
                  gCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCgg                                                                                                                           >  5:383882/1‑149 (MQ=255)
                   cAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCgg                                                                                                                           >  2:112472/1‑148 (MQ=255)
                    aaaGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCa                                                                                                                         >  4:325552/1‑149 (MQ=255)
                         gTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGa                                                                                                                    <  8:129304/149‑1 (MQ=255)
                          tttttCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGaa                                                                                                                   >  3:18956/1‑149 (MQ=255)
                               ccAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTc                                                                                                               <  1:297637/148‑1 (MQ=255)
                                cAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCg                                                                                                              <  1:168875/148‑1 (MQ=255)
                                 aGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCATTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCAGCATGCGAATGTCGCt                                                                                                            <  7:34921/149‑1 (MQ=255)
                                 aGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCt                                                                                                            >  6:384787/1‑149 (MQ=255)
                                   gAAGTCATCAGCCCCGGCGCGCATCGCCTGCAGCGCCAGCGGCACATGGCCATGCCCGCTAATCAGCAGCAGCGGGAGTTCGCTGTCGCGGCCGTGCAGTTGTTGGAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCt                                                                                                            <  5:282801/147‑1 (MQ=255)
                                         aTAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCCTCGATG‑CCCGGCATGCGAATGTCGCTGAccacca                                                                                                    >  2:358192/1‑149 (MQ=255)
                                         aTAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATc                                                                                                                                     <  7:57529/117‑1 (MQ=255)
                                         aTAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGAAAATGCA‑GGCCATCGATG‑CCCTCCATGCGAAAGTCGCTGAccacca                                                                                                    >  8:265491/1‑149 (MQ=255)
                                              ccccGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCg                                                                                               >  4:308490/1‑149 (MQ=255)
                                              ccccGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCg                                                                                               >  4:331763/1‑149 (MQ=255)
                                              ccccGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCg                                                                                               >  6:217883/1‑149 (MQ=255)
                                               cccGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCgg                                                                                              >  8:243951/1‑149 (MQ=255)
                                                ccGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCggg                                                                                             >  2:353866/1‑149 (MQ=255)
                                                  ggCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATGCCCCGGCATGCGAATGTCGCTGACCACCACGCCGGGc                                                                                            <  1:244657/149‑1 (MQ=255)
                                                   gcgcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGc                                                                                            >  7:12384/1‑147 (MQ=255)
                                                   gcgcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACAGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGc                                                                                            >  7:7002/1‑147 (MQ=255)
                                                    cgcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGac                                                                                                         >  5:30263/1‑133 (MQ=255)
                                                    cgcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGac                                                                                                         <  6:30263/133‑1 (MQ=255)
                                                     gcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGt                                                                                        <  1:112472/149‑1 (MQ=255)
                                                     gcgcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGt                                                                                        >  6:204860/1‑149 (MQ=255)
                                                       gcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCg                                                                                      <  3:331763/149‑1 (MQ=255)
                                                       gcATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCg                                                                                      <  1:353866/149‑1 (MQ=255)
                                                                 cACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATa                                                                             <  2:190483/148‑1 (MQ=255)
                                                                  aCCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCg                                                                                <  1:153522/144‑1 (MQ=255)
                                                                  aCCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCg                                                                                >  2:153522/1‑144 (MQ=255)
                                                                  aCCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACg                                                                           >  5:342624/1‑149 (MQ=255)
                                                                    cGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCt                                                                         >  3:293550/1‑149 (MQ=255)
                                                                     gCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTc                                                                        >  1:328381/1‑149 (MQ=255)
                                                                        aGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGc                                                                     <  3:169025/149‑1 (MQ=255)
                                                                         gCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGc                                                                     >  1:253082/1‑148 (MQ=255)
                                                                            gCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAgg                                                                 >  3:174791/1‑149 (MQ=255)
                                                                              acaTCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGcc                                                               >  1:7301/1‑149 (MQ=255)
                                                                                 tCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTg                                                            <  4:287355/149‑1 (MQ=255)
                                                                                 tCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTg                                                            <  3:308490/149‑1 (MQ=255)
                                                                                 tCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCACGCCTTg                                                            >  2:23950/1‑149 (MQ=255)
                                                                                     cATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCt                                                        >  2:85010/1‑149 (MQ=255)
                                                                                     cATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCt                                                        >  6:108906/1‑149 (MQ=255)
                                                                                      aTGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCtt                                                       >  7:248206/1‑149 (MQ=255)
                                                                                       tGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTc                                                      <  4:18956/149‑1 (MQ=255)
                                                                                         gCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCg                                                     <  5:63968/148‑1 (MQ=255)
                                                                                          ccGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGGGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGc                                                   >  6:93928/1‑149 (MQ=255)
                                                                                           cGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGc                                                   >  7:337227/1‑148 (MQ=255)
                                                                                                aTCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAgcgc                                             <  2:328381/149‑1 (MQ=255)
                                                                                                aTCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAgcgc                                             <  8:248206/149‑1 (MQ=255)
                                                                                                aTCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAgcg                                              <  3:325552/148‑1 (MQ=255)
                                                                                                 tCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTGCAGCAACTGCA‑GGCCCACGATG‑CCCGGGATGCGCATTTAGCTGACCCCCACGCCCGGCCACTCGGCCGCGATAAGCTCGGCCCGGCCGTGGGCTTAGGCCAGcgcg                                            >  4:185397/1‑149 (MQ=255)
                                                                                                 tCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCAACCCAG‑CCCCGCAAGCGAATGTCACTGCCCACCACCCCGGGCCAGTCGGCCGCGAAACCCTAGGCCCCGCCATGGGCTTCGGCCcgcgcg                                            >  8:5724/1‑149 (MQ=255)
                                                                                                    gcagcaCCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACt                                         <  5:217883/149‑1 (MQ=255)
                                                                                                          ccGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTg                                   <  2:7301/149‑1 (MQ=255)
                                                                                                           cGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCTGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGa                                  <  7:243951/149‑1 (MQ=255)
                                                                                                           cGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGa                                  <  5:179207/149‑1 (MQ=255)
                                                                                                           cGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGa                                  <  5:204860/149‑1 (MQ=255)
                                                                                                             gCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTc                                                                                       <  8:192005/94‑1 (MQ=255)
                                                                                                             gCAGTTATCTGTCGCGGCCGTGCAGTTGTTCCATAAACTGCA‑GGACATAGATG‑CACGGCATGCGAATGTCGCTGACCAACACCCCGGGCCAGTc                                                                                       >  7:192005/1‑94 (MQ=255)
                                                                                                              cAGTTCGCTGTCGCGGCCGTTCAGTTGTTCCCGCAACTGCA‑GGCCCTAGATG‑CCCGGCATGCGAATTTCGCTGACCACCACGCCGGGGCAGTCGGGCTCGATACGCTCGGCCCGGGCTTGGGCGTCGGGCCGCGCGCCTACCGTGAGcc                               >  6:44057/1‑149 (MQ=255)
                                                                                                                 ttCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGGCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCgg                            <  5:384787/149‑1 (MQ=255)
                                                                                                                        tCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTc                     <  7:265491/149‑1 (MQ=255)
                                                                                                                            ggCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAgg                 <  2:253082/149‑1 (MQ=255)
                                                                                                                              ccGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGt               <  8:337227/149‑1 (MQ=255)
                                                                                                                               cctGCAGTTGCTGGAACAACTGCACGGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGt               >  7:108506/3‑149 (MQ=255)
                                                                                                                                ctGCAGTTGCTGGAACAACTGCACGGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTc              >  2:21399/2‑149 (MQ=255)
                                                                                                                                ctGCAGTTGCTGGAACAACTGCA‑GGCCATCGATG‑CCCGGCATGCGTATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCt             >  1:264707/2‑149 (MQ=255)
                                                                                                                                     gttgttCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCt         >  5:32676/1‑148 (MQ=255)
                                                                                                                                     gttgttCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCt         >  6:274433/1‑148 (MQ=255)
                                                                                                                                     gttgttCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCt         >  1:14295/1‑148 (MQ=255)
                                                                                                                                      ttgttCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCa       <  5:93928/149‑1 (MQ=255)
                                                                                                                                         ttCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAg     <  1:332773/148‑1 (MQ=255)
                                                                                                                                           ccAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAGGCt  >  8:221606/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                      
TCGAGCAGGGCGTCTGTGGCAAAGGGTTTTTCCAGGAAGTCATAAGCCCCGGCGCGCATCGCCTGCACCGCCAGCGGCACATCGCCATGGCCGGTAATCAGCAGCACCGGCAGTTCGCTGTCGCGGCCGTGCAGTTGTTCCAGCAACTGCA‑GGCCATCGATG‑CCCGGCATGCGAATGTCGCTGACCACCACGCCGGGCCAGTCGGCCTCGATACGCTCGGCCAGGCCTTGGGCTTCGGCCAGCGCGACTACCTTGAGCCCGGCCAGGTCCAGGGTCTGGCTCAAGGCT  >  NC_002947/317572‑317859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑