| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,929,798 | A→T | 23.4% | intergenic (+21/+17) | PP_3464 → / ← PP_5603 | hypothetical protein/FAD dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,929,798 | 0 | A | T | 23.4% | 56.6 / 14.7 | 34 | intergenic (+21/+17) | PP_3464/PP_5603 | hypothetical protein/FAD dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (12/14); new base T (2/6); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CCACCTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCC > NC_002947/3929651‑3929943 | ccaccTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGaa < 1:183394/149‑1 (MQ=255) accTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAagc < 8:61617/149‑1 (MQ=255) ccTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAagca < 1:346011/149‑1 (MQ=255) ccTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCAG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAagca < 3:251039/149‑1 (MQ=255) ctgctgATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAagcag > 3:21997/1‑149 (MQ=255) ctgATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccc < 6:51191/149‑1 (MQ=255) aTCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGTATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACTTCCTGGGGCTGCGAAGCAGcccca < 1:130647/148‑2 (MQ=255) aTCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGCTCCGACATCCTGGGGCTGCGAAGCAGCCCCgg > 3:275903/1‑149 (MQ=255) aTCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGcccca < 5:239416/148‑2 (MQ=255) aTCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGcccca < 8:64543/148‑2 (MQ=255) tCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccag < 5:347075/148‑3 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAAtctc > 5:121453/1‑139 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAAccc > 1:22026/1‑149 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCCGGGGCTGCTTCGCAGccccagg > 3:224100/1‑130 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCCGGGGCTGCTTCGCAGccccagg < 4:224100/133‑4 (MQ=255) tttCAACAAGCCGGAGCGC‑GTGGGGCCCATTTGTGGTCCGCTGCGGTTTGGGCGGGAAACGGGTAATAACCTCCATATGTTTGGCGGCACCTTTTTCTGATCCGAAATCCTGGGGCTGCGAACCAGCCCCGGCAATCTCAAACCAAccc < 2:290578/149‑1 (MQ=255) cTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTg > 4:181291/1‑149 (MQ=255) aCAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccagg > 2:267357/1‑125 (MQ=255) aCAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccagg < 1:267357/128‑4 (MQ=255) gCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTcccc > 3:150174/1‑149 (MQ=255) cAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACAGAGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCTCCATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTAAAACCAACCCCTGCTCCACCAGCCAccg > 6:200325/1‑149 (MQ=255) aatctgggTACGCTGCTGGGTGGGGGGTAAACCGGGACTTACCTGCATTTGATTGTCGGGACCTTTTTCTGATCCGACTTCCTGGGGCGGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGctc < 4:302932/143‑1 (MQ=255) tGGTACGCAGCGGGGTGGGCGGTATACCGGCACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTctg < 8:199614/149‑1 (MQ=255) cacttcGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAACCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTctg < 2:67578/144‑1 (MQ=255) ggtgggCGGGATCCGGGGACTTACCTCCAATTGATTGGCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGACGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCa < 2:22026/149‑1 (MQ=255) gCGGTATACCGGCACTTACCTGCAATGGATGGCCGGGCCCTTTTTCTCACCCCACACCCTGGGGCTCCGAACCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGccc < 8:70738/149‑1 (MQ=255) gggATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAAACAAAACCTGCTACCCCAGCCCCACCCGCACGCTCCACTGCGGGTAACCCGCAAGcccc > 5:349675/1‑148 (MQ=255) aCCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTctgctg > 1:10143/1‑117 (MQ=255) ccacttcattgTCGGCAGATTTTTCTGATCCGACATCCTGTGCCTGCGAACCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACa < 4:275903/140‑1 (MQ=255) tgTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCACCCACCGCCGCACGCGCCTCTGCGGTTGACCCGCAAGCCCCGCCCATACCCCCTCCACAGGCCGcccc > 6:311820/1‑146 (MQ=255) gACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTTCCCCAGCCACCCCCGCACGCGCATCTGCAGTACACCCCCAAGGCCCGACAAAACCGCCACAACAGGCCCCCCAcgccgttt > 1:158367/1‑142 (MQ=255) gatttttCTCTACCTACATCCTGGGGCTGCTAACGAGCCCCTGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTAttt < 5:200325/147‑1 (MQ=255) ccTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAg < 4:150174/149‑1 (MQ=255) cTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTGCCCCAGCCACCGCCGCACGGTCCGCTGGGGTTGCCCCGCAAGCCCCGGCACTACCGCCGCCAACGGCCGCCCCCTGGGTATTTGTCGCCCCACCCGCGCCAGt > 2:195330/1‑149 (MQ=255) cGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCAcccc < 6:349675/148‑1 (MQ=255) | CCACCTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCC > NC_002947/3929651‑3929943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |