Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,936,285 C→G 5.9% A24G (GCC→GGC)  PP_3469 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,936,2850CG 5.9% 214.8 / 3.7 68A24G (GCC→GGC) PP_3469hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base G (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

CAACTGTCAAATCTATCAGTCGCCCAAGAGATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAACACCTCCTTACCGCAGGCT  >  NC_002947/3936142‑3936430
                                                                                                                                               |                                                                                                                                                 
cAACTGTCAAATCTATCAGTCGCCCAAGAGATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAgc                                                                                                                                               <  3:267729/148‑1 (MQ=255)
      tCAAATCTATCAGTCGCCCAAGAGATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGt                                                                                                                                        <  6:31458/149‑1 (MQ=255)
        aaaTCTATCAGTCGCCCAAGAGATATTTCTCGACCATCCTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGtt                                                                                                                                       <  5:140426/148‑1 (MQ=255)
          aTCTATCAGTCGCCCAAGAGATATTTCTCGACCATACTCCAGCCAAAACATCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGttctt                                                                                                                                    >  2:23785/1‑149 (MQ=255)
                          agagATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGGGCTACGTTCTTCCGTGACAGCTCCgta                                                                                                                    <  5:340052/149‑1 (MQ=255)
                          agagATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCgta                                                                                                                    <  8:53953/149‑1 (MQ=255)
                           gagaTATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCgtag                                                                                                                   <  4:46339/149‑1 (MQ=255)
                             gaTATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCgtagta                                                                                                                 <  3:162915/149‑1 (MQ=255)
                                atTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTg                                                                                                              >  4:77965/1‑149 (MQ=255)
                                  ttCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCg                                                                                                                      <  5:3079/139‑1 (MQ=255)
                                  ttCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCg                                                                                                                      >  6:3079/1‑139 (MQ=255)
                                         ccATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAatta                                                                                                     >  5:282560/1‑149 (MQ=255)
                                           aTACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAattatt                                                                                                   <  5:98897/149‑1 (MQ=255)
                                            tACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGACAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtg                                                                                                  >  5:164488/1‑149 (MQ=255)
                                              cTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtgtg                                                                                                <  3:77965/149‑1 (MQ=255)
                                              cTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtgt                                                                                                 <  6:282560/148‑1 (MQ=255)
                                               tCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGGCAGCGCttgt                                                                                                                                         <  4:1191/107‑4 (MQ=255)
                                               tCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGGCAGCGCttgt                                                                                                                                         >  3:1191/1‑104 (MQ=255)
                                               tCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtgtgt                                                                                               >  5:177311/1‑149 (MQ=255)
                                               tCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtgtgt                                                                                               >  4:213997/1‑149 (MQ=255)
                                                 cAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATtgtgtg                                                                                              >  2:305475/1‑148 (MQ=255)
                                                   gCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAg                                                                                           <  1:337795/149‑1 (MQ=255)
                                                   gCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACATCTACATCCAAACGCACTTCCACTCGGTGCTCACAAGCGCTGCCAGCGCAACGTTCTACCGTCACACCTCCGTAGTACTGGCCGAATTAATGTCTGAAg                                                                                           >  3:338102/1‑149 (MQ=255)
                                                       aaaCCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCaa                                                                                       <  8:22065/149‑1 (MQ=255)
                                                           cTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAAt                                                                                      >  5:279234/1‑146 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTAc                                                                                                                >  7:160361/1‑107 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTAc                                                                                                                <  8:160361/107‑1 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGaa                                                                                                        <  3:193179/115‑1 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGaa                                                                                                        >  4:193179/1‑115 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGAcc                                                                          <  6:279234/145‑1 (MQ=255)
                                                                        aaTGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTCTGTGAAGCCAATCAGACAGCGACCACGc                                                                      <  1:209926/149‑1 (MQ=255)
                                                                          tGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCtt                                                                    >  8:4692/1‑149 (MQ=255)
                                                                                gCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGc                                                              >  7:136271/1‑149 (MQ=255)
                                                                                    gcagcaTCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGctct                                                           >  8:160151/1‑148 (MQ=255)
                                                                                     cagcaTCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATGAGACAGCGACCACGCTTGTAGGCTCTGa                                                         <  1:23785/149‑1 (MQ=255)
                                                                                         aTCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATg                                                     >  3:23943/1‑149 (MQ=255)
                                                                                         aTCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATg                                                     <  8:136271/149‑1 (MQ=255)
                                                                                            tACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGc                                                                                          <  1:129336/109‑1 (MQ=255)
                                                                                            tACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGc                                                                                          >  2:129336/1‑109 (MQ=255)
                                                                                             aCGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGc                                                   >  1:299158/1‑147 (MQ=255)
                                                                                              cGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGcc                                                  <  6:177311/147‑1 (MQ=255)
                                                                                              cGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGc                                                   >  3:339529/1‑146 (MQ=255)
                                                                                               gACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGa                                               <  7:139211/149‑1 (MQ=255)
                                                                                                 cacTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGAt                                              >  4:364034/1‑148 (MQ=255)
                                                                                                 cacTTCTACAAGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCTCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATc                                             <  8:159838/149‑1 (MQ=255)
                                                                                                    ttCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCAcc                                          <  7:160151/149‑1 (MQ=255)
                                                                                                      cTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGTCCACGCTTGTAGGct                                                             <  2:128958/128‑1 (MQ=255)
                                                                                                      cTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCAccc                                         <  3:213997/148‑1 (MQ=255)
                                                                                                          aTGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAAc                                    >  4:17290/1‑149 (MQ=255)
                                                                                                              aTACGCACTTACTGTGGTTGGTGAGAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTTCTGGCCGAATTATTGTGTGAATCCAATCAGACAGCGACGACGCTTGTAGTGTCTGACATGTCCAGATCACCCATAACgcgc                                <  4:338102/149‑1 (MQ=255)
                                                                                                               tACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCg                                 >  4:46036/1‑147 (MQ=255)
                                                                                                               tACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCa                               <  3:364034/149‑1 (MQ=255)
                                                                                                                   cACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTAc                           <  7:4692/149‑1 (MQ=255)
                                                                                                                        acacTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAg                                                                                           <  5:264508/80‑1 (MQ=255)
                                                                                                                        acacTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAg                                                                                           >  6:264508/1‑80 (MQ=255)
                                                                                                                        acacTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGaa                       <  3:17290/148‑1 (MQ=255)
                                                                                                                          acTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAac                    >  7:274359/1‑149 (MQ=255)
                                                                                                                           cTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAaca                   >  1:149094/1‑149 (MQ=255)
                                                                                                                            tCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAacac                  >  2:348129/1‑149 (MQ=255)
                                                                                                                            tCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAATACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAacac                  >  1:280522/1‑149 (MQ=255)
                                                                                                                               gTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAACAcctc               <  2:299158/149‑1 (MQ=255)
                                                                                                                                ttaacaCAACCGCTGCCAGCGCTACGTTCTTCCGAGACAGATCCATAGTACTGGACGAAAAATAGTCTGAAGAAAATCAGACAACGACAACGCTTGTAGGCTCTGACAAGGCCAGAACACAAATAACCCGCAGAACCGAAAACAcatc               >  4:328552/6‑148 (MQ=255)
                                                                                                                                   tGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAACACCTCCTTa           >  6:58931/1‑149 (MQ=255)
                                                                                                                                    gACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCAcc                                          <  4:192455/117‑1 (MQ=255)
                                                                                                                                    gACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCAcc                                          >  3:192455/1‑117 (MQ=255)
                                                                                                                                       taGCGCTGGCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGaaaa                     >  2:75484/2‑135 (MQ=255)
                                                                                                                                       taGCGCTGGCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGaaaa                     <  1:75484/134‑1 (MQ=255)
                                                                                                                                            cTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGAGATGGCCAGATCACCCATAACCCGCAGTACGGAAAACACGTCCTTACCGCAGGCt  <  4:23943/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CAACTGTCAAATCTATCAGTCGCCCAAGAGATATTTCTCGACCATACTCCAGCCAAAACCTCGGTAGATCTCAATGACCAGCCCGCAGCATCTACGACACTTCTACATGCATACGCACTTACACTCGGTGCTGACAAGCGCTGCCAGCGCTACGTTCTTCCGTGACAGCTCCGTAGTACTGGCCGAATTATTGTGTGAAGCCAATCAGACAGCGACCACGCTTGTAGGCTCTGACATGGCCAGATCACCCATAACCCGCAGTACGGAAAACACCTCCTTACCGCAGGCT  >  NC_002947/3936142‑3936430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑