Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,504,056	(A)6→5	intergenic (+23/‑240)	rpsI → / → petA	30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,504,051	0	A	.	100.0%	184.2 / NA	41	intergenic (+18/‑245)	rpsI/petA	30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/22);  total (19/22)

TACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTT  >  NC_002947/1503908‑1504184                                                                                                                                                |                                                                                                                                      tACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGaaaaa                                                                                                                                   >  2:117590/1‑148 (MQ=255)      cgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTAATCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCg                                                                                                                             <  5:43606/148‑1 (MQ=255)           cccTGCGTGGCGCTCTGCGTCGTGCGGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTcc                                                                                                                        <  8:83055/148‑1 (MQ=255)             cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCtt                                                                                                                      <  1:121255/148‑1 (MQ=255)             cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCtt                                                                                                                      >  3:74544/1‑148 (MQ=255)             cTGCGTGGCGCGCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGGGGGTCTGCGTACAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCtt                                                                                                                      <  5:118114/148‑1 (MQ=255)              tGCGTGGCGCTCTGCGGCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTg                                                                                                                     <  8:28399/148‑1 (MQ=255)                          tGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCttt                                                                                                         >  2:168182/1‑148 (MQ=255)                             gtcgtGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCAAGGAACCGGGCttt                                                                                                         <  4:92475/145‑1 (MQ=255)                                 tGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa                                                                                                  <  4:101305/148‑1 (MQ=255)                                   cTGGCTACGTCACTCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGc                                                                                                <  3:59839/148‑1 (MQ=255)                                    tGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGCGGCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCACGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGcc                                                                                               <  3:56661/148‑1 (MQ=255)                                              aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAAc                                                                                     >  4:67556/1‑148 (MQ=255)                                                ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCt                                                                                   >  1:84614/1‑148 (MQ=255)                                                       gCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagca                                                                            <  3:98169/148‑1 (MQ=255)                                                           gTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtca                                                                        >  6:72409/1‑148 (MQ=255)                                                                  ggAGCTTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCTTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAACCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGcc                                                                  <  4:132143/146‑1 (MQ=255)                                                                    aGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGc                                                                <  2:18321/147‑1 (MQ=255)                                                                       gTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGt                                                            >  5:43185/1‑148 (MQ=255)                                                                            aaaGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAt                                                       <  1:13301/148‑1 (MQ=255)                                                                                    tCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc                                                   >  4:73220/1‑144 (MQ=255)                                                                                         gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATc                                            <  5:72409/146‑1 (MQ=255)                                                                                              gcgcGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCaaa                                     <  3:67556/148‑1 (MQ=255)                                                                                                    aaGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  4:90402/148‑1 (MQ=255)                                                                                                    aaGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  1:117590/148‑1 (MQ=255)                                                                                                    aaGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  2:84614/148‑1 (MQ=255)                                                                                                        gTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTg                           >  1:186296/1‑148 (MQ=255)                                                                                                              aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGATCTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGCTCAAGCAAAGCGTGGGTTGTCGccc                     >  5:107062/1‑148 (MQ=255)                                                                                                              aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGccc                     >  1:15413/1‑148 (MQ=255)                                                                                                                tACTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCtt                   <  1:168182/148‑1 (MQ=255)                                                                                                                tACTCCAAGCGCTAATACCGCTTCGGCACTCG‑AAAAAGCCCGCTTCCTTTGAACCCGGCTATTTTTTTAGACATGAAATAAACTGTCAGCAGTCAGTGGACGCATGTCGCATAAACCAACATCAAGCAAAGCGTGGGTTGTCGCCCtt                   >  2:171741/1‑148 (MQ=255)                                                                                                                  cTCCAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCCGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGc                 >  7:42322/1‑148 (MQ=255)                                                                                                                    cgaaGCGGTATTAACGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg               >  2:28522/3‑148 (MQ=255)                                                                                                                     cAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgg              >  2:68014/1‑148 (MQ=255)                                                                                                                     cAAGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCa                                           >  2:124074/1‑118 (MQ=255)                                                                                                                     cAAGCGTTAATACCGCTTCGGCAGTCG‑‑AAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg             >  6:103492/1‑148 (MQ=255)                                                                                                                      aaGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg             >  7:26785/1‑148 (MQ=255)                                                                                                                      aaGCGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCaa                                          <  1:124074/118‑1 (MQ=255)                                                                                                                         cGTTAATACCGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTa          >  2:23450/1‑148 (MQ=255)                                                                                                                                  cGCTTCGGCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCtt  <  1:171741/147‑1 (MQ=255)                                                                                                                                        ggCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCAcc    >  1:29477/1‑139 (MQ=255)                                                                                                                                         gCAGTCG‑AAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCt   <  2:29477/139‑1 (MQ=255)                                                                                                                                                |                                                                                                                                      TACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTT  >  NC_002947/1503908‑1504184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑