| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 95.7% | 64.6 / ‑2.6 | 23 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (10/12); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932499‑1932774 | tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGGCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGTGGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGC‑TTGCCCGCGaa < 3:78191/148‑1 (MQ=18) ctgctaaGAGGATCTGCACATTCGACAGCGGCATTTCAGGTCTAACGAAAATAGCAGTAAACGCTGGGGACGCTTTGCGGCCCTGCGCGGGCATGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGTAGCGGGC‑TTGCCCGCGAAg < 6:173055/143‑1 (MQ=255) tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCa > 7:132882/1‑148 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTAAAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAACGGCAGCAAGGccccc > 2:66879/1‑143 (MQ=38) ttCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTg < 1:6336/119‑1 (MQ=255) caccgCGGAATTGCCGGTCTCACTAAAATAGCCGTAAACGCGGGGGCCGCTTGGCGGCCCTGCGCGGGCACGCCCGCTCCTACAACGGATACGCGGCCCCTGTCGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 8:3910/143‑1 (MQ=25) ccgCGGCATTGCAGGGCTCACTAAAATAGCAGTAACCGGGGGGGCGGCTTTGCGGCCCGGCGCGGGAAAGCCCGCTCCAACAACGCATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:111802/146‑1 (MQ=25) cAGCGGCATTGCAGGGCGCACGAAAATAGCAGTAACCGCGGGGGCCGCTTGGCGGCCCTTCGCGGGCACGCCCGCTCCTACAACGGATAGGCGGCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 1:14/148‑1 (MQ=37) cAGCGGCAGTGCAGGGCCCACGAAAATCCCAGAAAACGCGGGGGCCGCTTTGCGGCCCGTCGCGGGCAACCCCGCTCCAACAAAGGATATGCGGCCCCTGAAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 3:166530/148‑1 (MQ=37) cAGCGGAATTCCAGGTCTCACTAAAATAGCCGTAAACGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCACGCCCGCTCATACAACGGATAGGGGCCCCGTGTCGGCGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 6:161411/148‑1 (MQ=25) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa > 5:82634/1‑148 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGCGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTTTCGACTACGCGGATCAGCg > 8:80389/1‑148 (MQ=38) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCCCGCCTAAAAAGGATATGCGGCCCCAGTAGGAGCGGGCGTTC‑CCCCCAAAGGCTGCAAGGCAGCCCCCAAATTTGGACAAACCCGATCAGCgct > 2:68683/1‑146 (MQ=255) tttGCGGCCGTGCGGGGGCACGCCCGCTCCTACAAAGTATAGGCGGCCCCTGTCGGCGGGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 6:113772/148‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 1:79904/1‑148 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGATCCAGAATAGAGCAGCTGCCCTGGTTTTCAAg > 3:57402/1‑148 (MQ=39) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 1:175626/1‑148 (MQ=255) aGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac > 4:152216/1‑148 (MQ=255) ggATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 4:61849/1‑148 (MQ=255) ggATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca < 2:175626/148‑1 (MQ=255) gAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca < 3:19326/148‑1 (MQ=255) gAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 2:28602/147‑1 (MQ=255) gAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 6:82634/147‑1 (MQ=255) gAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATAAGCGATCCGGCAGGCAGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTACAGCTCGATATGCACATCATCGTTGCCGATGtta > 2:114421/1‑146 (MQ=39) | TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932499‑1932774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |