Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,988,8090GA30.0% 43.5 / 19.0 31intergenic (+103/‑443)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (6/15);  new base A (5/4);  total (11/20)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCG  >  NC_002947/5988668‑5988953
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gttccgggcctggtaggtgttcgccgggcgagtttcagcggctgggaggtgttcgccgggagtgtttcagcgccggggagagcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTc                                                                                                                                            <  8:91678‑M2/51‑1 (MQ=255)
     gctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCtgtg                                                                                                                                       <  1:62751/148‑1 (MQ=255)
                  tttGATTAGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGACTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACCCCCAcgcg                                                                                                                          >  5:158790/1‑148 (MQ=255)
                     gATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGc                                                                                                                       >  1:16406/1‑148 (MQ=255)
                      aTTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGcc                                                                                                                      >  5:36503/1‑148 (MQ=255)
                           gATGCGTACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTGGTAGGGCCTGTGAGATGGGGCGCCGCCCGCGCGGCGCTGCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCt                                                                                                                  <  7:23050/147‑1 (MQ=37)
                             tGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGGTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc                                                                                                               <  7:46837/148‑1 (MQ=255)
                                         ctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGat                                                                                                   >  3:175321‑M2/57‑148 (MQ=255)
                                              tGGCGGGTTGCTTGATGGTTGTAGTTCCTGTTATATTGGTCGCCGCCCGGGCGGCGCTTCGCGTGTAAACCCGCTTCTACGTTTGTTTAGGGGCATTTATTCCTGTTGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATc                                                                                                <  6:158790/146‑1 (MQ=255)
                                              aggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATcgc                                                                                              <  7:10043‑M2/97‑1 (MQ=255)
                                                            agagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAg                                                                                <  6:5644‑M2/111‑1 (MQ=255)
                                                            aGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaacaa                                                                                 <  2:16406/147‑1 (MQ=255)
                                                             gggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAgg                                                                               <  1:147261/148‑1 (MQ=255)
                                                               ttttgggcgcccaggagatcgagcgccgcccgcgCGGCGCTTCGCGGGGAAACCCGCTCCTACGGTTGTTGCGGGCCAGTTCTTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg                                                                             <  1:172769‑M2/114‑1 (MQ=255)
                                                                     gTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaacaa                                                                                 >  8:14448/1‑138 (MQ=255)
                                                                      tGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg                                                                                <  7:14448/138‑1 (MQ=255)
                                                                      cgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc                                                                      <  5:151213‑M2/121‑1 (MQ=255)
                                                                             gagaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTg                                                               <  1:192474/148‑1 (MQ=255)
                                                                                aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGGCGCGCGCGCCTGGca                                                            <  3:160873/148‑1 (MQ=255)
                                                                                              gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc                                              <  2:7889‑M2/145‑1 (MQ=255)
                                                                                                            gggTAAACCCGCGCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTa                                <  1:140198/148‑1 (MQ=255)
                                                                                                                   cccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg                         <  6:36503/148‑1 (MQ=255)
                                                                                                                    ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg                        >  4:70101/1‑148 (MQ=255)
                                                                                                                     cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt                       >  3:9651/1‑148 (MQ=255)
                                                                                                                        tCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc                   >  4:92607/1‑148 (MQ=11)
                                                                                                                         ccTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAc                   <  3:8515‑M2/148‑2 (MQ=255)
                                                                                                                              gtttgtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATAGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcgc               >  4:175829/1‑146 (MQ=2)
                                                                                                                                   tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAATCAAGGCGGTCGCGCGCGACTGGTGCAGGCGGGACTGGCC‑GAACCACACGTAGGAGCGGGTTTACCCGCGAAgcgc        >  7:133840/1‑148 (MQ=255)
                                                                                                                                        ggCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGCGCGGGTTTCcccgcgaagcgccgcg    <  7:69514‑M2/148‑17 (MQ=255)
                                                                                                                                         gCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCcgcgcg  >  3:36674/1‑148 (MQ=14)
                                                                                                                                          ccAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcg  <  8:126626‑M2/148‑19 (MQ=255)
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GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCG  >  NC_002947/5988668‑5988953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑