Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,580,567 A→T V277D (GTC→GAC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,5670AT100.0% 112.7 / NA 36V277D (GTC→GAC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/16);  total (20/16)

ATACCGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATG  >  NC_002947/1580424‑1580698
                                                                                                                                               |                                                                                                                                   
aTACCGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTTCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGc                                                                                                                                 <  6:152674/148‑1 (MQ=255)
  aCCGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTGGCCGTTGAACTGGGCGCTGTCGGc                                                                                                                                 >  3:38501/1‑146 (MQ=255)
    cGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTa                                                                                                                              <  6:140001/147‑1 (MQ=255)
    cGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAg                                                                                                                             >  8:68224/1‑148 (MQ=255)
             tCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCAccc                                                                                                                    >  2:114598/1‑148 (MQ=255)
             tCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCAccc                                                                                                                    >  5:592/1‑148 (MQ=255)
             tCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTATACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCAccc                                                                                                                    >  8:155477/1‑148 (MQ=255)
             tCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTACAGCGCGTTGGCGACGGTTGCCAGTTTTACCGCCAGGCAGGAAGACGGCTTGCCGTTGAACTGGTCGCTGTCGGCGTAGTGTTAAccc                                                                                                                    >  7:98485/1‑148 (MQ=255)
               ccGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCTGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCAccccc                                                                                                                  >  4:138885/1‑148 (MQ=255)
                cggcggGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCAccccc                                                                                                                  <  4:102006/147‑1 (MQ=255)
                      gaagaaCGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCt                                                                                                           >  5:98582/1‑148 (MQ=255)
                      gaagaaCGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCt                                                                                                           >  3:9290/1‑148 (MQ=255)
                       aagaaCGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTa                                                                                                          >  5:11238/1‑148 (MQ=255)
                          aaCGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACtt                                                                                                       >  4:87516/1‑148 (MQ=255)
                            cGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTgg                                                                                                     <  4:43267/148‑1 (MQ=255)
                             ggTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTggg                                                                                                    >  8:71101/1‑148 (MQ=255)
                             ggTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTggg                                                                                                    >  2:22314/1‑148 (MQ=255)
                                           gATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAgg                                                                                      >  1:187958/1‑148 (MQ=255)
                                                    gcgcAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCtg                                                                              >  2:57391/1‑147 (MQ=255)
                                                             cTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCt                                                                    <  3:177287/148‑1 (MQ=255)
                                                              ttGGCGGGGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCtt                                                                   <  5:127052/148‑1 (MQ=255)
                                                                 gCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCttt                                                                  <  7:71101/146‑1 (MQ=255)
                                                                 gCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTttg                                                                 <  6:592/147‑1 (MQ=255)
                                                                     tgtCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGAc                                                            <  2:19417/148‑1 (MQ=255)
                                                                     tgtCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGAc                                                            <  2:125557/148‑1 (MQ=255)
                                                                          aGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGGACCGTCTTTGTTGACCTTGa                                                       >  1:26525/1‑148 (MQ=255)
                                                                          aGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGa                                                       <  2:129325/148‑1 (MQ=255)
                                                                                ttGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGa                                                 <  6:20477/148‑1 (MQ=255)
                                                                                      ccGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCTCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATActct                                           <  1:95417/148‑1 (MQ=255)
                                                                                             ccAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTg                                    >  8:33373/1‑148 (MQ=255)
                                                                                              cAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCACAGGCGCACCTGTGACCCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGc                                   >  2:119357/1‑148 (MQ=255)
                                                                                                        gCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGttt                         >  4:13277/1‑148 (MQ=255)
                                                                                                         ccAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGttt                         >  3:81869/1‑147 (MQ=255)
                                                                                                          cAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGc                       <  4:9290/148‑1 (MQ=255)
                                                                                                                              cGTTGAACTTGGCGCTGTCGGCGTAGTTTTCACCCACAAGGCCTACTTTGGCGACATCTCCCAGGCTCAACTGTGAACCGTCTTTGTTGACCTTTAGCAGTATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCgatgat   <  1:101023/148‑1 (MQ=255)
                                                                                                                               gTTGAACTGGGCGCTGTCGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCACCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGatgatg  <  1:114598/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                   
ATACCGCCTTGACTCCCGGCGGGAAGAACGGTTCCAGGCCTTTGATGGTCTCGCGCAGTGCCTTGGCGGTGTCCAGCGCGTTGGCGCCGGTTGCCAGTTTTACCGCCAGGCCGGAAGCCGGCTTGCCGTTGAACTGGGCGCTGACGGCGTAGTTTTCACCCCCCAGGCCTACTTGGGCGACATCGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATG  >  NC_002947/1580424‑1580698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑