Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,843,971	C→T	A275T (GCG→ACG)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,971	0	C	T	100.0%	109.5 / NA	33	A275T (GCG→ACG)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

GCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCA  >  NC_002947/1843833‑1844108                                                                                                                                           |                                                                                                                                          gCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAg                                                                                                                                  <  5:45854/148‑1 (MQ=255) gCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAg                                                                                                                                  <  1:65804/148‑1 (MQ=255)                   cGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGAttt                                                                                                                 >  4:25402/1‑147 (MQ=255)                     cTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTc                                                                                                                <  1:26298/146‑1 (MQ=255)                      ttttttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                             <  5:156704/148‑1 (MQ=255)                      ttttttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                             <  2:154030/148‑1 (MQ=255)                      ttttttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                             <  5:12481/148‑1 (MQ=255)                              agcagGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGGTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCa                                                                                                     >  5:71466/1‑148 (MQ=255)                                 agGTGTGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGt                                                                                                  <  3:136965/148‑1 (MQ=255)                                   gTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTc                                                                                                >  5:159343/1‑148 (MQ=255)                                                        aTTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGcc                                                                           >  1:120643/1‑148 (MQ=255)                                                            aGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTgg                                                                        <  1:31662/147‑1 (MQ=255)                                                             gCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCGCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGt                                                                       <  7:142462/147‑1 (MQ=255)                                                              cGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCg                                                                     >  7:67661/1‑148 (MQ=255)                                                                      ggATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCtgtg                                                             >  6:74096/1‑148 (MQ=255)                                                                            cGTGGCTCATGTTGGCCAGGAACTCCGACTTGAAGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCTATTGTTTCCAGGTTCTGTCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCa                                                        <  3:24740/147‑1 (MQ=255)                                                                                 cTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGGTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGttctt                                                  <  8:30749/148‑1 (MQ=255)                                                                                  tCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGttcttc                                                 >  6:90377/1‑148 (MQ=255)                                                                                     tggtGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTg                                              <  7:36092/145‑1 (MQ=255)                                                                                     tGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGt                                               <  5:67190/147‑1 (MQ=255)                                                                                     tGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTg                                              <  8:116502/148‑1 (MQ=255)                                                                                            cAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGGGTTGCAGTTCTTCGTGGGCATTc                                       >  8:162193/1‑148 (MQ=255)                                                                                                aaCTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCa                                   <  1:105107/148‑1 (MQ=255)                                                                                                 aCTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGTTGGCGGGGGCGAGGCTGTGTTGCTTTTCTACGTGGGCATTcgcctg                                  >  6:6602/1‑143 (MQ=255)                                                                                                 aCTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAg                                  <  5:169911/148‑1 (MQ=255)                                                                                                   tCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAggg                                >  1:37227/1‑148 (MQ=255)                                                                                                     cGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGtt                              >  3:25440/1‑148 (MQ=255)                                                                                                             tGCGGCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCAt                      <  1:89106/148‑1 (MQ=255)                                                                                                                 gCTGGCCTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCgg                  <  3:134546/148‑1 (MQ=255)                                                                                                                       cTCAAGTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATg            >  5:160263/1‑148 (MQ=255)                                                                                                                            gTGCCTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCACATCTTCGAGCGCATACACCACGGTATCCCCCATCCGGATGATGCCGtc       >  3:95616/1‑147 (MQ=255)                                                                                                                               ccTCTTTGCGCGTCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGCTGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGc    >  3:99347/1‑148 (MQ=255)                                                                                                                                 tctTTGCGCGTCACGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCa  >  3:25211/1‑148 (MQ=255)                                                                                                                                           |                                                                                                                                          GCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCA  >  NC_002947/1843833‑1844108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑