Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,788,028	T→A	K203* (AAG→TAG)	PP_3350 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,788,028	0	T	A	100.0%	104.6 / NA	31	K203* (AAG→TAG)	PP_3350	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/9);  total (22/9)

CAAAGGCTAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTTGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGTATAGCGCCCGGCGGC  >  NC_002947/3787887‑3788169                                                                                                                                              |                                                                                                                                              cAAAGGCTAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGGTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGcgtcc                                                                                                                                         >  5:37225/1‑148 (MQ=255) cAAAGGCTAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACAGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGcgtca                                                                                                                                         >  2:68293/1‑147 (MQ=255)       cTAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCtt                                                                                                                                   <  7:68852/148‑1 (MQ=255)        tAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCt                                                                                                                                    >  3:138278/1‑146 (MQ=255)        tAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCt                                                                                                                                    >  4:50876/1‑146 (MQ=255)                 cATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCttgaggttgagg                                                                                                                         >  7:141384/1‑148 (MQ=255)                 cATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCttgaggttgagg                                                                                                                         >  6:3564/1‑148 (MQ=255)                        cACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGgcgc                                                                                                                  >  3:179232/1‑148 (MQ=255)                          cgacgCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGgcgcgc                                                                                                                <  8:68128/148‑1 (MQ=255)                              gCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGc                                                                                                            >  5:7119/1‑148 (MQ=255)                                cGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGGTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCg                                                                                                          <  7:128737/148‑1 (MQ=255)                                             gTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGc                                                                                             <  5:4713/148‑1 (MQ=255)                                                            tAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGt                                                                              >  1:89443/1‑148 (MQ=255)                                                            tAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGt                                                                              >  1:86703/1‑148 (MQ=255)                                                                        ttgttgttgCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGa                                                                  >  6:121157/1‑148 (MQ=255)                                                                        ttgttgttgCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGa                                                                  >  7:146849/1‑148 (MQ=255)                                                                                gctgcCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACAcc                                                          >  6:132928/1‑148 (MQ=255)                                                                                      aTCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCg                                                    >  2:26947/1‑148 (MQ=255)                                                                                             cAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAg                                              <  5:3564/147‑1 (MQ=255)                                                                                              aaCTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGc                                             >  6:136194/1‑147 (MQ=255)                                                                                                    cTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCTTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCAtc                                        <  4:138278/146‑1 (MQ=255)                                                                                                       cACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTg                                   >  8:152092/1‑148 (MQ=255)                                                                                                         ccGGTTTTCAGCGGTATCACAGCGAATGCATCCAGATAGCGTCCGTCCTTGAGTTTGAGTATTGAGCGCACGCATTCTAAGCTCATGCGGATGTTCGGCGAGTCGCGGTAGGTGACCAACACCTTGTCGCCGAATCCCAGCTCCTgg                                  >  5:119395/1‑147 (MQ=255)                                                                                                          cGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCg                                >  5:164347/1‑148 (MQ=255)                                                                                                                   gCGGGCGCACAGCGAATGCATCCAGCTAGCGGCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTAcgc                        <  5:99947/147‑1 (MQ=255)                                                                                                                          cacaGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGta                <  8:141384/148‑1 (MQ=255)                                                                                                                           acaGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGt                 >  8:121075/1‑146 (MQ=255)                                                                                                                            caGCGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGtata              <  4:78570/148‑1 (MQ=255)                                                                                                                               cGAATGCATCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGGGTGGTATAgcg           >  7:166771/1‑148 (MQ=255)                                                                                                                                      aTCCAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGTATAGCGCCcggcg    >  4:142082/1‑148 (MQ=255)                                                                                                                                         cAGCTAGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGTATAGCGCCCggcggc  >  7:21254/1‑147 (MQ=255)                                                                                                                                              |                                                                                                                                              CAAAGGCTAGCAGGTCCATGTTCCACGACGCCGACAGCGGCAAGGTGCCGTAGAGGCCGTAGAATTTGATGTTGTTGTTGCTGCCATCGTCCCAACTGTCTTCACCGGTTTTCAGCGGGCGCACAGCGAATGCATCCAGCTTGCGTCCGTCCTTGAGGTTGAGGCTGGCGCGCACGCCGTCGAAGCTCAGGCGGATGTTCGGCGCGTCGCGGTAGGTGACCAACACCTGGTCGCCGAAGCCCATCTCCTGGCGACCTACGCGTGTGGTATAGCGCCCGGCGGC  >  NC_002947/3787887‑3788169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑