Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	91.1 / NA	27	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/12);  total (15/12)

CCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740683‑4740953                                                                                                                                      |                                                                                                                                          ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACTCCGGCGAGCAGACGCGCGACAGCGGTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGTCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                            <  1:60494/148‑1 (MQ=255)    tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                         <  8:71805/148‑1 (MQ=255)       aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                       <  8:24424/147‑1 (MQ=255)         gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                                    <  7:151929/148‑1 (MQ=255)          aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                   <  7:77691/148‑1 (MQ=255)           tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGACGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCt                                                                                                                  >  5:135906/1‑148 (MQ=255)                       gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                     <  4:11723/148‑1 (MQ=38)                        ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                     <  1:161800/148‑1 (MQ=255)                          aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                                   >  7:9288/1‑148 (MQ=255)                                 cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAAGAGGCCGGcaca                                                                                            >  7:33662/1‑148 (MQ=255)                                  acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTAGTCCTGTGCCGGCCTCATCGCCGCCATGCCCCCCCAGAGGCCGGCACAg                                                                                           >  3:86075/1‑148 (MQ=255)                                                    gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGACACCACAAAACCTCAGt                                                                         >  3:73534/1‑148 (MQ=255)                                                           ttGCCCTTAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                                   <  2:25919/147‑1 (MQ=255)                                                            ggCCCTGAAGGCCCGCCAGTACGCTGCAGGCGCCGAACGCAAGACGGCTGCCCTCGGGCTG‑CGTTTTTTATTTGTCCTGGGCCGGCCTCTTGGCGGCGTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                                 <  1:11930/147‑1 (MQ=255)                                                                cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TGTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTTCCCGCGCAGAGGCCGGCACAGACACAACAAAACCGAAGTTTTTCTccgccc                                                             >  8:82444/1‑148 (MQ=255)                                                                 tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACCGCAGTTTTTCTccgcccg                                                            >  3:139136/1‑148 (MQ=255)                                                                         cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGACGCCGGCCCAGCCACCCCAAAACCGCAGTTTTTCTCCGCCCGCTCACGCa                                                    >  8:100356/1‑148 (MQ=255)                                                                                    ggCAGTAGCCGAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGCCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                         <  1:20645/147‑1 (MQ=255)                                                                                        gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTTCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGTTTTTGTCCGCCCGCGCACGCAGCCCTTTCAGGGTAt                                     >  2:139137/1‑148 (MQ=255)                                                                                             cGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCCCAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                                >  3:83060/1‑148 (MQ=255)                                                                                                  taaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGGAGAGGCCGGCCCAGCCACCACAAAACCTCAGTTTTTATCCCCCCGCTGACCCACCCCTTTGAGGGGATTGAACGgcgc                           >  6:50021/6‑148 (MQ=255)                                                                                                    aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATACCCCCGCTCACGCACCCCTTTCAGGGTATTGAAAGGCGCAt                         >  8:37092/1‑148 (MQ=255)                                                                                                              cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCAACCACAAAACCTCAGTTTTTCTACGCCCCCTCACGCAGCCCTTTAAGGGTATTTAACGGCGCATCCACCACGa                >  4:114283/1‑147 (MQ=255)                                                                                                                   gggCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgt          <  7:72027/148‑1 (MQ=255)                                                                                                                      cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTgg       <  4:132054/148‑1 (MQ=255)                                                                                                                            ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGGCGGCCCAGCCACCACAAAACCTCAGTTTTTCTGCGCCCGCTCACGCAGCCCTTTCAGGGTATTGCACGGCGCATCCACCACGAACTTGTTGGccacc  >  5:171602/1‑148 (MQ=255)                                                                                                                            ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGCAGCGGCCGGCCCAGCCCCCCCCAAACCACAGTTTTTATCCGCCCGCTCACGCAGCCCGTTCAGGGTATTGGACGGCGCCTCCACCCCGCACTTGTTGGcccc   >  2:148081/1‑145 (MQ=255)                                                                                                                                      |                                                                                                                                          CCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740683‑4740953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑