Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	57.5 / NA	18	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCC  >  NC_002947/499060‑499323                                                                                                                                                |                                                                                                                         gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCa                                                                                                                      >  1:12078/1‑148 (MQ=255)    cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGCTTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGc                                                                                                                   <  7:4665/148‑1 (MQ=255)             ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACg                                                                                                          <  6:161517/148‑1 (MQ=255)                  aGGCACGCCTGATGCAGAAGTTGACAGTGAACTCGCTGGCGGAGCGGGGGAAGCACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                     <  3:26674/148‑1 (MQ=255)                    gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCTGCCCGCGGACcgggcagg                                                                                                   >  3:172557/1‑143 (MQ=255)                              tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGa                                                                                           <  2:134975/146‑1 (MQ=255)                                                aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                        <  6:124591/147‑1 (MQ=255)                                                     cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGCCAGGCCTGCTGATCTCTATTGCTGCGCCGGccgattc                                                                  >  5:58148/1‑143 (MQ=255)                                                         cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                              <  3:159406/148‑1 (MQ=255)                                                                 gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCGGCGTATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCCTGccc                                                      >  3:132649/1‑148 (MQ=255)                                                                 gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggcc                                                                                                 >  5:75140/1‑105 (MQ=255)                                                                  tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggcct                                                                                                <  6:75140/105‑1 (MQ=255)                                                                       tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGCCGGGCCGGCGGATCTCTATGGCTGCGCCGGCCGCTTCGCGGGCATGCCCGCGccc                                                >  3:137361/1‑148 (MQ=255)                                                                                     caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGCCGGGCCTGCTGATCTCTATGGCTGGGCCGGGCTCTTAGCGGGCATGCCCGCGCCCACAGGTTtggtggg                                  >  7:51929/1‑147 (MQ=255)                                                                                        gcggatctgttTCCTACATCGGGGTCCCGTAAACTTGCCGACGCCTTTGGGGGCGCGGCCAGGCCGGCAAAGGGGCCGGGCGTGCTGATCTCTTTGGGTGCGCGGGCCTGTTCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTgg                               <  4:20053/145‑1 (MQ=255)                                                                                                              ggTGCCCTAAACTTGCCGATGCCTTTGTGGGTGGGGGCATGCCCGCGAAGGGGCCTGGCCTGCTGATCTCTTTGGCTGAGCGGGCCTCTTCGCGGGAATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc         <  4:137361/148‑1 (MQ=255)                                                                                                               gTGCCCTAAACTTGCCTATGCCTATTTGGTTGCGGGCATGCCCGCTAACGGGCGTGTCCTGCTTATTTATATTGCTGAGCCGTCATTTTCGGGGTATTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCTCAAGGGCATTCCAATCCCg        <  6:168705/148‑1 (MQ=255)                                                                                                                     gAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTTATATATATTGCTGAGCCGGCCACATACCGGGCATTCCCCCTACCACACCTTTAGATGTTGTCAAGCAAGGGCATTACACTACCGcccaac  >  8:164735/1‑144 (MQ=255)                                                                                                                                                |                                                                                                                         GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCC  >  NC_002947/499060‑499323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑