Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,987,204	G→A	V96M (GTG→ATG)	kefB‑III →	glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,987,204	0	G	A	100.0%	102.5 / NA	31	V96M (GTG→ATG)	kefB‑III	glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/12);  total (19/12)

ACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCAGAGCCTGGCCGAGAGCAAGCAGCT  >  NC_002947/5987059‑5987346                                                                                                                                                  |                                                                                                                                               aCTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAatg                                                                                                                                              <  8:30889/148‑1 (MQ=255)      gCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGc                                                                                                                                          <  1:133280/147‑1 (MQ=255)        cTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGa                                                                                                                                       <  3:90772/148‑1 (MQ=255)                cgACACAGAAAGCGGGGCGCACATTTCCGAACTGGGCGTGGGCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGGGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCa                                                                                                                               <  8:56120/148‑1 (MQ=255)                      aGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATc                                                                                                                         >  1:160793/1‑148 (MQ=255)                       gAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCg                                                                                                                        <  5:46234/148‑1 (MQ=255)                       gAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGACCAAATGGTGCTGACCGGCGCAGTCATCg                                                                                                                        >  3:108784/1‑148 (MQ=255)                                   cacaTTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGccc                                                                                                            >  2:137542/1‑148 (MQ=255)                                    acaTTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCt                                                                                                           >  6:94723/1‑148 (MQ=255)                                    acaTTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCt                                                                                                           >  6:126254/1‑148 (MQ=255)                                               cTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCa                                                                                                <  7:9258/148‑1 (MQ=255)                                                   gCGTTGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCGTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCAATGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGc                                                                                              <  4:129521/146‑1 (MQ=255)                                                         tCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCt                                                                                      <  6:12191/148‑1 (MQ=255)                                                          cTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCATCAGCCAGACGCTa                                                                                     >  6:11747/1‑148 (MQ=255)                                                           ttGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGCCGCTa                                                                                     >  6:103076/1‑147 (MQ=255)                                                                              gCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCg                                                                  <  1:94306/147‑1 (MQ=255)                                                                                   gAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTgg                                                            >  3:116668/1‑148 (MQ=255)                                                                                            cccAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGgcgc                                                   >  6:88933/1‑148 (MQ=255)                                                                                             ccAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCt                                                  >  2:23151/1‑148 (MQ=255)                                                                                              cAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCtt                                                 >  4:47942/1‑148 (MQ=255)                                                                                                aaCGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTc                                               >  6:104949/1‑148 (MQ=255)                                                                                                  cGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCAGCGGCGACATCGCCCTGTTCGGCTTAATCCATACGCTACCAGCAGCCATAGTGCTCGGTCTGGGCCTGGCGCTTTCGt                                             >  6:120418/1‑148 (MQ=255)                                                                                                            tGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCt                                   >  4:148019/1‑148 (MQ=255)                                                                                                            tGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCt                                   >  7:167217/1‑148 (MQ=255)                                                                                                                gcgcAAGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGc                               <  3:92941/148‑1 (MQ=255)                                                                                                                     aGTCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCACCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCt                             >  7:57695/1‑145 (MQ=255)                                                                                                                       tCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCaga                        <  2:85164/148‑1 (MQ=255)                                                                                                                       tCGGTGTTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCaga                        <  6:156222/148‑1 (MQ=255)                                                                                                                           tgtTTGGTGTCGGCACTGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCAGAGCCt                    <  1:137542/148‑1 (MQ=255)                                                                                                                                 gtgtCGGCACTGACCAAATGGTGCTGAACGGCGCAGTCATCGGCGCCATCGCACTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTAGGTCTGGGCCTGGCGCTTTCGTCCCCCGCCCTTGGCCTGCAGCGCCTGGCCga              >  3:98320/1‑148 (MQ=255)                                                                                                                                           tGCCCAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGTCGCTTTCGTCCACCGCACTTGGCCTGCAGAGCCTGGCCGAGAGCAagcag    >  8:123054/1‑148 (MQ=255)                                                                                                                                              ccAAATGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCAGAGCCTGGCCGAGAGCAAGCAGCt  >  1:20779/1‑147 (MQ=255)                                                                                                                                                  |                                                                                                                                               ACTTGGCCTGATCCGCGACACAGAAAGCGTGGCGCACATTTCCGAACTGGGCGTGGTCTTGCTGCTGTTCATCATCGGCCTGGAACTGTCGCCCAAACGCTTGTGGCTGATGCGCAAGTCGGTGTTTGGTGTCGGCACTGCCCAAGTGGTGCTGACCGGCGCAGTCATCGGCGCCATCGCCCTGTTCGGCTTCAGCCAGACGCTACCAGCAGCCATCGTGCTCGGTCTGGGCCTGGCGCTTTCGTCCACCGCCCTTGGCCTGCAGAGCCTGGCCGAGAGCAAGCAGCT  >  NC_002947/5987059‑5987346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑