| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 97.4% | 103.0 / ‑3.1 | 38 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/22); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499361‑1499649 | gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑cg‑cc < 6:99762/149‑1 (MQ=37) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCcac < 3:67479/149‑1 (MQ=25) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTg > 6:111453/1‑149 (MQ=21) cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCAt < 7:19469/148‑1 (MQ=21) cgcgCCTGAACGACGCCTGGGCACCACCGCCGCACTGAGGCTGCCAAACGGGGGGAGCGGGCGGGCCCGCTAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTa < 6:290773/146‑1 (MQ=18) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 1:250671/147‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 1:69053/147‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCCCCAAACAAGCGCCCCCGTGAATAGCACCGGCTTTGCCCGTTTTAGCGGGCA‑AGCCCCCACCCAAAGACAGATTGCCAATAATACGGCCTa > 6:173123/1‑148 (MQ=255) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTTCCCGCGCAACAGGCGCCCCGGTGCCTGGCCCCCGCTTTGCCGGTGTTAGCGGGCACCC‑CCGCTCCCCCAGACTGATTGCCATTAAGCGGGCCAAACGc > 8:11052/1‑149 (MQ=255) tgCACCACTGCCGGACTGAGGCTGCCAAACGGTGGGCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGTTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 8:378391/146‑1 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 6:311490/148‑1 (MQ=21) accagAGCAGGACTGAGGCTGCAAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGGGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 8:67243/145‑1 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTTCCATTAAGCGGGCCTATCGCTACAgag > 7:67243/1‑146 (MQ=21) tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACAGATTGc > 4:278491/1‑109 (MQ=14) tGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt > 6:310461/1‑149 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 7:382367/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:322591/147‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 5:173123/149‑1 (MQ=21) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt < 5:111453/149‑1 (MQ=21) gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 1:359594/101‑1 (MQ=14) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:344539/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGGCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:33198/149‑1 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc < 2:239954/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 7:3315/1‑149 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc > 1:97757/1‑98 (MQ=17) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc < 2:97757/98‑1 (MQ=17) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:38293/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 4:201731/1‑121 (MQ=21) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 3:201731/121‑1 (MQ=21) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:66306/1‑147 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 5:310461/149‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGAA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATAATGACTCACTTAGAACGCTATc > 2:339625/1‑147 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCGATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 4:254975/1‑149 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTCTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 7:14080/146‑1 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTATTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc > 8:14080/1‑146 (MQ=37) gCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtc < 7:113478/148‑1 (MQ=255) ccGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt > 3:353954/1‑149 (MQ=255) tttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 3:250335/143‑1 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg > 5:112715/1‑149 (MQ=255) | GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499361‑1499649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |