Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	211.9 / NA	62	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (34/28);  total (34/28)

AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG  >  NC_002947/307719‑308004                                                                                                                                                    |                                                                                                                                             aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑cc                                                                                                                                            <  5:382160/149‑2 (MQ=255) aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑cc                                                                                                                                            <  5:376242/149‑2 (MQ=255)   ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                          >  1:329270/1‑146 (MQ=255)        ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCt                                                                                                                                      <  5:138274/149‑1 (MQ=255)         tctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCt                                                                                                                                      >  3:371560/1‑148 (MQ=255)           tcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGAt                                                                                                                                   >  1:36914/1‑149 (MQ=255)           tcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGACCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGAt                                                                                                                                   >  7:229208/1‑149 (MQ=255)             ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATc                                                                                                                                  <  4:212996/148‑1 (MQ=255)                gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGt                                                                                                                              >  4:28141/1‑148 (MQ=255)                     aCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTgacgac                                                                                                                        <  4:371560/149‑1 (MQ=255)                        tCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAg                                                                                                                     >  3:150002/1‑149 (MQ=255)                             ctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACgg                                                                                                                <  2:329270/149‑1 (MQ=255)                                ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                        >  5:273018/1‑123 (MQ=255)                                ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                        <  6:273018/123‑1 (MQ=255)                                ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGttt                                                                                                             <  3:28141/149‑2 (MQ=255)                                       ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGaa                                                                                                      <  6:407897/149‑1 (MQ=255)                                        cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                     >  4:100321/1‑149 (MQ=255)                                             tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                <  4:150002/149‑1 (MQ=255)                                             tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                <  4:301851/149‑1 (MQ=255)                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                               >  8:136735/1‑148 (MQ=255)                                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                             <  3:100321/147‑1 (MQ=255)                                                     aTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                        >  5:204811/1‑149 (MQ=255)                                                                  ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                           <  2:36914/149‑1 (MQ=255)                                                                   cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                          >  8:40302/1‑149 (MQ=255)                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTggg                                                                                                           >  4:87375/1‑115 (MQ=255)                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTggg                                                                                                           <  3:87375/115‑1 (MQ=255)                                                                     aCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCGTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                     <  7:206547/136‑1 (MQ=255)                                                                     aCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCATTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                     >  8:206547/1‑136 (MQ=255)                                                                      cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                          >  1:352713/1‑79 (MQ=255)                                                                      cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                          <  2:352713/82‑4 (MQ=255)                                                                             gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                           <  6:219885/138‑1 (MQ=255)                                                                             gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                           >  5:219885/1‑138 (MQ=255)                                                                                 aagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTg                                                             >  7:271650/1‑148 (MQ=255)                                                                                    gCGATGTGACCTTGTTCTTCGGCCTGACCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATc                                                         <  8:229208/149‑1 (MQ=255)                                                                                         gtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                            >  2:219452/1‑125 (MQ=255)                                                                                         gtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                            <  1:219452/125‑1 (MQ=255)                                                                                            aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                 >  7:331960/1‑149 (MQ=255)                                                                                                tGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCAGCTGACGAAAGCCGTTACCTGATC‑GGTgac                                                                                                                           <  3:342009/71‑1 (MQ=255)                                                                                                tGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTATCAGCTGACGAAAGCCGTTACCTGATC‑GGTgac                                                                                                                           >  4:342009/1‑71 (MQ=255)                                                                                                 gTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                            <  4:353317/101‑1 (MQ=255)                                                                                                 gTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                            >  3:353317/1‑101 (MQ=255)                                                                                                   tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                           <  8:271650/148‑1 (MQ=255)                                                                                                     ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                        >  6:362948/1‑149 (MQ=255)                                                                                                      tcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACgg                                                                                                                >  6:287536/1‑76 (MQ=255)                                                                                                      tcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACgg                                                                                                                <  5:287536/76‑1 (MQ=255)                                                                                                      tcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                       >  8:44430/1‑149 (MQ=255)                                                                                                        ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa                                     <  5:362948/149‑1 (MQ=255)                                                                                                         gCCTGTCCGGGAATGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAATGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                <  2:68993/137‑1 (MQ=255)                                                                                                         gCCTGTCAGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCAAGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                >  1:68993/1‑137 (MQ=255)                                                                                                              tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCg                                               <  5:256942/133‑1 (MQ=255)                                                                                                              tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCg                                               >  6:256942/1‑133 (MQ=255)                                                                                                                             aCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                <  7:40302/149‑1 (MQ=255)                                                                                                                               tACCCTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc               >  1:26365/1‑148 (MQ=255)                                                                                                                                 cccTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg            >  7:214793/1‑149 (MQ=255)                                                                                                                                  ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg            >  1:48121/1‑149 (MQ=255)                                                                                                                                  ccTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACg                                      >  8:207415/1‑122 (MQ=255)                                                                                                                                  ccTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc               <  1:215678/145‑1 (MQ=255)                                                                                                                                  ccTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc               >  2:215678/1‑145 (MQ=255)                                                                                                                                   cTTTCGGCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCa          >  6:396612/1‑149 (MQ=255)                                                                                                                                         gCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                         <  4:37613/128‑1 (MQ=255)                                                                                                                                         gCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                         >  3:37613/1‑128 (MQ=255)                                                                                                                                         gCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                >  4:182715/1‑137 (MQ=255)                                                                                                                                         gCTGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                <  3:182715/137‑1 (MQ=255)                                                                                                                                           tGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  >  1:173927/1‑149 (MQ=255)                                                                                                                                           tGACGAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  >  2:341541/1‑149 (MQ=255)                                                                                                                                               gAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTggg                                                                                                           <  8:348128/40‑1 (MQ=255)                                                                                                                                               gAAAGCCGTTACCTGATC‑GGTGACGACGAGCACGGTTggg                                                                                                           >  7:348128/1‑40 (MQ=255)                                                                                                                                                    |                                                                                                                                             AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATC‑GGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG  >  NC_002947/307719‑308004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑