| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 97.2% | 109.7 / ‑5.0 | 37 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (19/16); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921322‑2921600 | cTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCg < 5:21742/148‑1 (MQ=255) gAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGa > 3:156323/1‑149 (MQ=255) aGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGAc < 5:194341/149‑1 (MQ=255) cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGCCGTGGGTACCGACACCCTGAG‑CAGGGGGGGCGGCAACGACTTcc > 8:343850/1‑149 (MQ=255) cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTcc < 4:156323/149‑1 (MQ=255) cGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACg > 2:295618/1‑149 (MQ=255) ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGTAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg > 2:304885/1‑149 (MQ=255) gtcgcTGGCGATGACTGCCTGGATGGCGGCTACGGCAATGACACGATGCAGTGAGGCGATGGTAACGACACATTGTTCGGCGAGGGGGGTACCGTCACCCTGAT‑CTGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa < 6:240795/147‑1 (MQ=255) gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGTCAACGATACggtggt < 7:343850/149‑1 (MQ=255) aCAGCCTGGATGGCGGCAACGACAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggt > 8:59396/1‑149 (MQ=255) cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCTGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg < 1:295618/149‑1 (MQ=255) aGCCTGGATGGCGGTAACGGGAATGCCACCCTGCAGGGCGGCGCTGGTAACGACACATTGTTGGGCGACGTGGGTACCGACACCCGGATCCGGGGGCGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg < 1:304885/149‑1 (MQ=255) gATGTCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTACGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg < 7:242923/149‑1 (MQ=255) caacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGaca > 1:117133/1‑149 (MQ=255) acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc < 2:117133/149‑1 (MQ=255) gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCaa > 6:146091/1‑97 (MQ=255) gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCaa < 5:146091/97‑1 (MQ=255) acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc < 3:137734/140‑1 (MQ=255) acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc > 4:137734/1‑140 (MQ=255) ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc > 1:194109/1‑148 (MQ=255) ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa < 1:158177/149‑1 (MQ=255) tGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATgtgt > 4:284555/1‑149 (MQ=255) gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc < 2:194109/149‑1 (MQ=255) acaTTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc > 1:57280/1‑149 (MQ=255) aTTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg < 2:200115/92‑1 (MQ=255) aTTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg > 1:200115/1‑92 (MQ=255) aTTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg > 3:178073/1‑149 (MQ=255) gTTCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCTGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAGCGATGTGTTCCAGTTCGCTGCa < 7:59396/148‑1 (MQ=255) ttCGGCGACGTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg < 4:178073/148‑1 (MQ=255) gTGGGTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa < 3:284555/149‑1 (MQ=255) gggTACCGACACACTTAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACAGATGGAAACGATGTGTTCAAGTTCGCTGAAGGCTTTGGCAAc > 1:25890/1‑148 (MQ=255) gTACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc < 2:57280/149‑1 (MQ=255) tACCGACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc > 7:52821/1‑149 (MQ=255) gACACCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat > 7:263966/1‑149 (MQ=255) acCCTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAGGACCTGatcat > 6:55931/1‑149 (MQ=255) ccTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc > 3:371630/1‑148 (MQ=255) ccTGAT‑CAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc > 3:317999/1‑148 (MQ=255) | CTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAT‑CGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921322‑2921600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |