Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG97.0% 221.2 / ‑1.7 66intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (26/38);  total (27/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCC  >  NC_002947/4740677‑4740950
                                                                                                                                           |                                                                                                                                      
tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                              >  5:215519/1‑149 (MQ=255)
      ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                        <  5:367725/149‑1 (MQ=255)
       cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                       <  7:70997/149‑1 (MQ=255)
        gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                      >  8:121569/1‑149 (MQ=255)
        gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTACCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                        >  5:220043/1‑147 (MQ=255)
        gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTACCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                        <  6:220043/147‑1 (MQ=255)
              gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                <  4:174355/149‑1 (MQ=255)
                tCGGCCGTCCGCGCCAGCTGTAGACCGGCGAGAAGAATCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                              <  7:107952/149‑1 (MQ=255)
                       tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                        >  2:281291/1‑148 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                                     <  5:414747/148‑1 (MQ=38)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     <  1:102051/149‑1 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     <  7:85740/149‑1 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     <  1:157406/149‑1 (MQ=255)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                   <  7:121569/149‑1 (MQ=38)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                   <  8:219154/149‑1 (MQ=38)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                   <  7:234761/149‑1 (MQ=255)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                   <  1:252649/149‑1 (MQ=255)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCCAGGCCGCGAagaa                                                                                                  >  3:212342/1‑148 (MQ=255)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                  <  7:313841/149‑1 (MQ=38)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                    <  4:212342/147‑1 (MQ=255)
                                gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                  <  3:180072/145‑1 (MQ=38)
                                gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                  >  4:180072/1‑145 (MQ=38)
                                 cTGTACCCCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTCTTCGCGGCCTTGCCCGCCAAGAGGCCgg                                                                                             >  2:277043/1‑149 (MQ=255)
                                  tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                   >  3:361009/1‑142 (MQ=255)
                                  tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                   <  4:361009/142‑1 (MQ=255)
                                       acCTGCGAGCAGAAGTGCTACATCGTTGCCCTGAAATACCGCAAGTAAGCTGCAGTAGCCGATCTCAAGATGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGACGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                      <  2:113039/134‑1 (MQ=255)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTTCACACCGGCCGCC‑AATTTTATTTGTCCTATGACGGCCTCTTCACGGCCTTGccaccca                                                                                                      >  1:113039/1‑129 (MQ=255)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                       >  5:109229/1‑149 (MQ=255)
                                        ccGGGGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCACTTAAGCGGCAGTAGCCGAACGCAAGATGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCATGGCCGCGAAGAGGCCGGCACAgga                                                                                      <  6:215519/149‑3 (MQ=25)
                                          ggCGACCAGAAGCGCTACATCGTGCCCCTCAAAGACCGCAAGTACGCGGCCGTAGCCGAACGCAAGAAGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGCCCTTCCCCGCTAAGAGGCCGGCACAGacac                                                                                    <  2:337282/149‑1 (MQ=255)
                                                cagccgcgcTACATCGGGGCCCTCAACGCCCCCACGTCAGCTGCCTTCGCCCAACCCACGACGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCGGTGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaa                                                                              <  8:73959/144‑1 (MQ=255)
                                                cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                    <  2:391193/111‑1 (MQ=255)
                                                cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                    >  1:391193/1‑111 (MQ=255)
                                                                ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                              <  1:281291/149‑1 (MQ=255)
                                                                   cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATccgcc                                                           >  3:173239/1‑149 (MQ=255)
                                                                    ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                          >  6:92598/1‑149 (MQ=255)
                                                                     cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                  <  4:333843/108‑1 (MQ=25)
                                                                     cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                  >  3:333843/1‑108 (MQ=25)
                                                                      tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGCCTTGCCCCCGAAGAGGCCGGCACAGACGCCACAAAACCTCAGTTTTTCTacgcccgc                                                        >  4:132136/1‑149 (MQ=255)
                                                                       gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCCGCCTTGCCCCCGAAGAGGCCGGCACAGCCACCACAAAACCTCAGATTTTATccgccccc                                                        >  7:373931/1‑146 (MQ=255)
                                                                            aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCAAAGGTTTTATACGCCCGCGCACg                                                   >  4:184881/1‑148 (MQ=255)
                                                                              cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     <  1:103411/96‑1 (MQ=38)
                                                                              cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     >  2:103411/1‑96 (MQ=38)
                                                                                cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                              >  8:119808/1‑149 (MQ=255)
                                                                                    tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                           >  8:378091/1‑148 (MQ=255)
                                                                                      cggtGCCGTAGCCTAACGCAACACGGCGGCCGTGGGGCAG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTCCCCGCTATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCTTTTCa                                        <  4:157361/146‑1 (MQ=255)
                                                                                      aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCCCGCGAACAGGCCCGCACAGACACCACAAAACCACAGTTTTTCTCCGCCCCCGCACGCCGCCCttt                                          >  3:302190/1‑147 (MQ=255)
                                                                                        ctccAGTTGCCCAACGAATGAAGGCTGCCCTCGGGAAG‑CTTTTTTTTTTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                      <  2:341692/146‑1 (MQ=255)
                                                                                        cTGCAGTAGCCGAACGCAACACGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTGGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                      <  8:373931/149‑1 (MQ=255)
                                                                                          gCAGTAGCCGAACGCAAGACCGCTGCCAGCGGGCAGCC‑TTTTTTATTTGTCGGGGGCCGCCCTCTTCGCGGCCTGGCCCGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGt                                    <  8:185074/149‑1 (MQ=255)
                                                                                           cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACAACACAAAACCTCAGTTTTTCTACGCCCGCGCACGCAGCCCGTTGAGGGtt                                   >  2:333679/1‑148 (MQ=255)
                                                                                             gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTGGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttttt                                                                  <  6:87231/116‑1 (MQ=38)
                                                                                             gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttttt                                                                  >  5:87231/1‑116 (MQ=38)
                                                                                                 ccGAACGCAAGAAGGCTGCCCGCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                             <  3:322880/149‑1 (MQ=255)
                                                                                                      cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                          >  6:112127/1‑147 (MQ=255)
                                                                                                      cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                          <  5:112127/147‑1 (MQ=255)
                                                                                                         aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                     <  7:378091/149‑1 (MQ=255)
                                                                                                         aaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGaata                            >  4:132401/4‑140 (MQ=255)
                                                                                                              ggCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCTAATAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                <  1:277043/149‑1 (MQ=255)
                                                                                                                   cccTCGTGCTGCCTTTTTTT‑TTTGTCGTGGGCCGCCCTCTTCCCGGCGTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc           <  4:302190/149‑1 (MQ=255)
                                                                                                                    ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCGTGGCCGCGAAGAGGCCGGGCCAGCCCCCACAAAACCACAGGTTTTATACGCCCGCGCCCCCCGCCCATTGAGGGTATTGAACGGCGCCGCCACCACGAAcg          >  4:391301/1‑148 (MQ=255)
                                                                                                                     cTCGGGCAG‑CTTTTTTTATTTGCCCTGTGCCGCCCTCTTCGGGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt         <  7:330487/149‑1 (MQ=255)
                                                                                                                        gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                       <  7:83788/116‑1 (MQ=38)
                                                                                                                        gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                       >  8:83788/1‑116 (MQ=38)
                                                                                                                         ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg     <  6:109229/149‑1 (MQ=255)
                                                                                                                             agccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcc  <  7:119808/145‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCC  >  NC_002947/4740677‑4740950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑