Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	122.8 / NA	37	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  NC_002947/499060‑499331                                                                                                                                                 |                                                                                                                                 gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                             >  8:11118/1‑149 (MQ=255)     aTCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                         <  2:318637/149‑1 (MQ=255)      tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                        >  8:52829/1‑149 (MQ=255)          cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                        <  8:372112/145‑1 (MQ=255)            cccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACg                                                                                                                  <  8:103281/149‑1 (MQ=255)             ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACg                                                                                                                  >  1:98991/1‑149 (MQ=255)             ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                 <  8:36734/149‑1 (MQ=255)               acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAGGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                                               <  5:172353/149‑1 (MQ=255)               acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAGGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                                               <  5:166468/149‑1 (MQ=255)                caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCCAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCACGCGAACGggcc                                                                                                              >  5:298614/1‑149 (MQ=255)                  aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                            <  1:32983/149‑1 (MQ=255)                      aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                             <  6:51333/128‑1 (MQ=255)                      aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                             >  5:51333/1‑128 (MQ=255)                                cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATctct                                                                                              <  7:11118/149‑1 (MQ=255)                                 aGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                            <  2:288909/118‑1 (MQ=255)                                 aGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATAGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                            >  1:288909/1‑118 (MQ=255)                                  gAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt                                                                                            <  2:98991/149‑1 (MQ=255)                                          aaGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                            <  4:247031/125‑1 (MQ=255)                                          aaGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                            >  3:247031/1‑125 (MQ=255)                                                    gCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                         <  4:226018/102‑1 (MQ=255)                                                    gCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                         >  3:226018/1‑102 (MQ=255)                                                    gCTGGCGGAGCG‑TGTGAAGTACGCCTTGGAGCTCAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTc                                                                          <  2:105213/149‑1 (MQ=255)                                                          ggAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc                                                                                         <  2:262744/128‑1 (MQ=255)                                                          ggAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc                                                                                         >  1:262744/1‑128 (MQ=255)                                                                  gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                      <  4:193460/92‑1 (MQ=255)                                                                  gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                      >  3:193460/1‑92 (MQ=255)                                                                  gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGCTCTCTATGGCTGAGCCGGCCTCATAGCGGGCATGCCCg                                                             >  8:9244/1‑149 (MQ=255)                                                                                  aGCACAAGATGATCTTATTGCGACATACGGTTGCCCCAAACTTGCCGACGCCTATGTGGGTGCGGGAATGCCCGCGAAGGGGCCTGGCCTGCTTATCTCTATGGCTGAGCCGGCCTCTTCGCGGGAATGCCCGCTCCCACAGGTTTCGt                                             <  6:298614/149‑1 (MQ=255)                                                                                           tgatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCAGGCATGc                                                                <  2:46179/121‑1 (MQ=255)                                                                                           tgatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCAGGCATGc                                                                >  1:46179/1‑121 (MQ=255)                                                                                             atctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTc                                    <  7:52829/147‑1 (MQ=255)                                                                                                tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCggg                                                                     >  6:144879/1‑111 (MQ=255)                                                                                                tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCggg                                                                     <  5:144879/111‑1 (MQ=255)                                                                                                     gCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAATCCCGCGAACGGGCCAGGCCAGCTGAACTCTATGGCAGAGCCGGCCACTTAGCCGGCATGCCCGCACCCACAGGTTTCGTGGTTGGCTCGCCAGGGGAt                          >  8:367618/1‑149 (MQ=255)                                                                                                             cGGGTGCCCGAAACTTGCCGAAGCCTCTGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCGGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                  <  7:367618/149‑1 (MQ=255)                                                                                                              gggTGCCCTAACCTTGCCCACCCCTTGGGGGCCGCGGCCAGGCCCGCCAACGGGCCGGGCCTGCTGATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCAGGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc                 <  2:337319/149‑1 (MQ=25)                                                                                                                             gCCGAAGCCTATGTGGGAGCGGGCACCCCCCCGAACGGGCCTGGCCTGCTGATCTGTATGGCTGAGCCGGCCTCTTAGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTTGCTAGCAAGGGGAGTGCAAAACCGCCATCCTTTTAggg  >  8:216622/1‑149 (MQ=37)                                                                                                                                                 |                                                                                                                                 GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCT‑GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  NC_002947/499060‑499331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑