| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 96.9% | 105.5 / ‑3.0 | 32 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (15/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCG > NC_002947/3845558‑3845843 | ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc‑g > 5:244247/1‑147 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gg > 7:209365/1‑147 (MQ=255) tgTCGGCATGATCACTTGAAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTACTGGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGAAGAAAATACGTGTTTC‑GC‑gggc > 3:120378/1‑144 (MQ=255) tgTCCGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑ggg < 4:114659/145‑2 (MQ=255) cGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGAt > 3:112490/1‑147 (MQ=255) cGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gggctga < 5:206849/146‑6 (MQ=255) tgatgaCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AA‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑AAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCAt < 4:120378/146‑1 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGAGTTGCGC‑‑‑AAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCg > 7:171832/1‑146 (MQ=255) aTCACGTGCAGGCTCTGCTAGAGATCGCGGTGGCGGCTCATTCATTGATTGTTATTGTTGTGATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCAt < 1:225543/146‑1 (MQ=255) tGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCa > 5:228261/1‑147 (MQ=255) aGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCTCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGTCAGAAAAGACGTGTTAC‑GCGGGCTGATCCATCGTCAGCCACg < 4:226732/146‑1 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGAt > 3:65698/1‑146 (MQ=255) ctctGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGa < 1:105724/144‑1 (MQ=255) ctctGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGa > 2:105724/1‑144 (MQ=255) ctctGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCCCCATCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGa < 1:219703/147‑1 (MQ=255) tGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATc < 4:112490/147‑1 (MQ=255) cAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAg > 2:69515/1‑147 (MQ=255) aaGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAg < 2:93888/146‑1 (MQ=255) ttCATTTATTGTTATTGTTGTCATCGCGGGCTCTCGGTCTGAAGCGTGGCGC‑‑‑CACACTAAGAGCTGGATGACGGGCATAAAACACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACTATGTATCAGCCCGCGCCCTGCCAGGCCg < 6:244247/145‑1 (MQ=255) tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag > 7:146806/1‑146 (MQ=255) tgttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg > 6:239628/1‑147 (MQ=255) gttgtCATCGCGGTCGCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTGC‑GCGGGCTGATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg < 7:197365/146‑1 (MQ=255) tgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtgg < 8:209365/147‑1 (MQ=255) ctgtctgAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag > 6:100678/1‑147 (MQ=255) aaGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg > 7:133555/1‑147 (MQ=255) gc‑‑‑CATACTATTAGCTGTTTGACGGCCATAAATGCCGTGTGTCGGG‑GGCTGCGCCTGCGTCCGCCACCATGTACATCCCCGCGCCCTGCCAGGCCTGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGATCgt < 8:171832/147‑1 (MQ=255) c‑‑‑cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtg > 7:132188/1‑147 (MQ=255) gagCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcg > 2:98812/1‑147 (MQ=255) cTGTATGACGTGCAGAAATGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgcg < 7:141400/146‑1 (MQ=255) gggCATAAAACACGTGTTTC‑GCGGGCTGATCCTTGGTCAGCCACTATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt < 6:57686/146‑1 (MQ=255) gCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc < 4:65698/147‑1 (MQ=255) aaaaGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt < 1:69515/147‑1 (MQ=255) cGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg < 6:245638/116‑1 (MQ=255) cGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg > 5:245638/1‑116 (MQ=255) cGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCa < 5:239628/147‑1 (MQ=255) tttC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCa > 3:132054/1‑143 (MQ=255) tttC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgcg > 7:7404/1‑147 (MQ=255) | CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCG‑AC‑TTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGC‑‑‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCG > NC_002947/3845558‑3845843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |