| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,456,793 | 1 | . | T | 20.0% | 32.0 / 10.0 | 15 | intergenic (+44/‑137) | gapB/PP_2150 | glyceraldehyde‑3‑phosphate dehydrogenase/thiamine biosynthesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base . (5/7); new base T (2/1); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGAATTCGGTTATAGCTGCCAGGTGGTTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGC > NC_002947/2456670‑2456933 | aCGAATTCGGTTATAGCTGCCAGGTGGTTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAAtgtgtg > 8:68662/1‑147 (MQ=255) gCTGCCAGGTGGTTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTa < 3:140798/119‑1 (MQ=255) gCTGCCAGGTGGTTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTa > 4:140798/1‑119 (MQ=255) ggtggtTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTcgc > 7:103639/1‑147 (MQ=255) gtggtTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTcgcg < 1:261329/147‑1 (MQ=255) tggtTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTcgcg < 8:103639/146‑1 (MQ=255) aGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTAc < 3:133855/147‑1 (MQ=255) aCATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCGTTTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACa > 3:229392/1‑147 (MQ=255) aCCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCGTTTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGATTATCGCTGcc < 4:229392/146‑1 (MQ=255) ccGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGATTATCGCTGCCCCTg < 1:209967/147‑1 (MQ=255) aTTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCAG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGCGTAAACCCGCAACAAAAGCATTATCGCTGACCCAGTAGGACCGGTTTTACCcgc > 8:63802/1‑146 (MQ=255) cTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCGACAGGATTATAGCTGGCCCGGTAGGAGCGGGTTTACCCGCGAagag > 4:15406/1‑147 (MQ=39) cAATGAAAAAACGGGAACCTTCGGGTTCCCGTTTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAAACCCCAACAACAAGATTATAGCGGGCCCTGTAGGGGCGGGTTTACCCGCGAAAAGGCCAGCAc > 4:5295/1‑147 (MQ=255) ggAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTTAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGaaa < 3:15406/147‑1 (MQ=255) gTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGTATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTgc < 7:68662/147‑1 (MQ=255) | ACGAATTCGGTTATAGCTGCCAGGTGGTTCGTGTGATGGAAGACATGGCTGGTGTGAACCCGCCAGCGTTTCCACGCTGATTCGCTTGTAAGGCAATGAAAAAACGGGAACCTTCGGGTTCCCG‑TTTTTTTTTATTCTGAATGTGTGTTGTCAGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGC > NC_002947/2456670‑2456933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |