Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,733,447	A→G	Y730H (TAT→CAT)	sucA ←	2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,733,447	0	A	G	100.0%	200.3 / NA	56	Y730H (TAT→CAT)	sucA	2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/31);  total (25/31)

GCAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCAT  >  NC_002947/4733303‑4733588                                                                                                                                                 |                                                                                                                                              gcAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTTAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGc                                                                                                                                             >  4:87629/1‑147 (MQ=255) gcAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGc                                                                                                                                             >  7:218262/1‑147 (MQ=255) gcAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGc                                                                                                                                             >  1:19258/1‑147 (MQ=255)      gggCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCgtgt                                                                                                                                        >  3:131231/1‑147 (MQ=255)       ggCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGtgtg                                                                                                                                       <  6:72738/147‑1 (MQ=255)        gCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCGTCATGGCCGtgtg                                                                                                                                       >  8:100752/1‑146 (MQ=255)           aaTGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGgcag                                                                                                                                   <  7:114457/147‑1 (MQ=255)            aTGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGgca                                                                                                                                    >  5:43899/1‑145 (MQ=255)                ccTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGgcagcagca                                                                                                                              >  8:232644/1‑147 (MQ=255)                ccTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGgcagcagc                                                                                                                               <  3:130283/146‑1 (MQ=255)                 cTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCAt                                                                                                                             <  6:40882/147‑1 (MQ=255)                     cGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTc                                                                                                                         <  5:99252/147‑1 (MQ=255)                            gcagGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGc                                                                                                                  <  8:200548/147‑1 (MQ=255)                             cagGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGca                                                                                                                 <  7:67943/147‑1 (MQ=255)                                gtggtAAATCTGTGCCTGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAAAGCTCCAGACGCGCGTAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAg                                                                                                              <  5:70721/147‑1 (MQ=255)                                    tAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAggcg                                                                                                           <  6:43899/146‑1 (MQ=255)                                      aaTCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGcc                                                                                                        >  3:115375/1‑147 (MQ=255)                                       ttCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGcc                                                                                                        <  6:37830/145‑1 (MQ=255)                                            tgCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACtt                                                                                                  >  1:246981/1‑147 (MQ=255)                                             gCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACtt                                                                                                  <  4:102816/146‑1 (MQ=255)                                             gCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACt                                                                                                   <  4:131231/145‑1 (MQ=255)                                              ccGGCGTCGTCGGTACGCGGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTtgt                                                                                                >  7:141066/1‑147 (MQ=255)                                               cGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTtgtg                                                                                               >  3:232111/1‑147 (MQ=255)                                                 gCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCt                                                                                             <  8:117528/147‑1 (MQ=255)                                                        cgGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACt                                                                                      <  4:263956/147‑1 (MQ=255)                                                           tACGCAGACCTGGATGTTGTGCTCGGCGCAGAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGt                                                                                   <  2:56868/147‑1 (MQ=255)                                                           tACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTgg                                                                                    >  8:107744/1‑146 (MQ=255)                                                               cAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGtgatg                                                                               <  4:232111/147‑1 (MQ=255)                                                                  aCCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAAc                                                                            <  7:232644/147‑1 (MQ=255)                                                                    cTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTg                                                                          <  8:141066/147‑1 (MQ=255)                                                                     tGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTgg                                                                         <  7:107744/147‑1 (MQ=255)                                                                     tGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTgg                                                                         <  1:121211/147‑1 (MQ=255)                                                                                   ggCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTca                                                                                           <  1:144931/115‑1 (MQ=255)                                                                                   ggCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTca                                                                                           >  2:144931/1‑115 (MQ=255)                                                                                           gCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATcac                                                                 <  4:2393/133‑1 (MQ=255)                                                                                           gCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATcac                                                                 >  3:2393/1‑133 (MQ=255)                                                                                             tGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTg                                                 >  7:122267/1‑147 (MQ=255)                                                                                                 ggTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGa                                             <  2:246981/147‑1 (MQ=255)                                                                                                   tAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAg                                           >  4:263465/1‑147 (MQ=255)                                                                                                        cTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGCCCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGAGCACCACTTGCGCACCGTTGGCGAAGTCGc                                       >  7:179277/1‑146 (MQ=255)                                                                                                        cTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCCCCGTTGGCGAAGTCGc                                       >  1:160146/1‑146 (MQ=255)                                                                                                         tCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCg                                     <  1:46542/147‑1 (MQ=255)                                                                                                            aGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAAc                                  >  6:199236/1‑147 (MQ=255)                                                                                                              aCGCGCGGAGGAGTGCTCCGGCCCCTGCCCTCCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTg                                >  5:258984/1‑147 (MQ=255)                                                                                                               cgcgcgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTgg                               <  6:29443/147‑1 (MQ=255)                                                                                                               cgcgcgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACAGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTg                                >  1:69189/1‑146 (MQ=255)                                                                                                                 cgcgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGCCCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGCACTGGTCGATCACCACTTGCGCCCCGTTGGCGAAGTCGCCGAACTGGGc                             >  7:147880/1‑147 (MQ=255)                                                                                                                 cgcgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGc                             <  4:258561/147‑1 (MQ=255)                                                                                                                   cgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTca                                                                                           >  1:123983/1‑83 (MQ=255)                                                                                                                   cgGAGGAGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTca                                                                                           <  2:123983/83‑1 (MQ=255)                                                                                                                    ggaggaGTGCTCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTc                          <  3:87629/147‑1 (MQ=255)                                                                                                                              tCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTgg                                                >  5:65445/1‑115 (MQ=255)                                                                                                                              tCCGGCCCCTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTgg                                                <  6:65445/115‑1 (MQ=255)                                                                                                                                   ccccTGCCCTTCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAgcgc           >  3:178938/1‑147 (MQ=255)                                                                                                                                           cTTCATGGCCGGGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCGTCCCAGATCACCAGCGCGTTCGGCa   <  8:122267/147‑1 (MQ=255)                                                                                                                                            ttCATGGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCAt  <  7:185710/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              GCAGCGGGCGAATGACCTGGCGACGCAGCAGGTGGTAAATCTGTGCCGGCGTCGTCGGTACGCAGACCTGGATGTTGTGCTCGGCGCACAGCTGCAGGTAACGCTCCAGACGCGCGGAGGAGTGCTCCGGCCCCTGCCCTTCATAGCCGTGTGGCAGCAGCATGGTCAGACCGCACAGGCGGCCCCACTTGTGCTCACCACTGGTGATGAACTGGTCGATCACCACTTGCGCACCGTTGGCGAAGTCGCCGAACTGGGCTTCCCAGATCACCAGCGCGTTCGGCAT  >  NC_002947/4733303‑4733588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑