Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 499,203 A→G intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947499,2030AG100.0% 105.1 / NA 30intergenic (+47/‑249)uhpA/PP_0411two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/19);  total (11/19)

CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCT‑CGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499062‑499346
                                                                                                                                             |                                                                                                                                                
ccATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACTCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                                             <  6:132588/147‑1 (MQ=255)
      gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                                        <  8:16992/146‑1 (MQ=255)
       cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                                      <  7:188922/147‑1 (MQ=255)
         cccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGaa                                                                                                                                    >  3:221998/1‑147 (MQ=255)
          ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGTTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAc                                                                                                                                   <  8:151424/147‑1 (MQ=255)
             caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACggg                                                                                                                                <  1:167623/147‑1 (MQ=255)
             caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGAACggg                                                                                                                                >  5:268467/1‑147 (MQ=255)
                          aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                                        <  4:144181/126‑1 (MQ=255)
                          aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                                        >  3:144181/1‑126 (MQ=255)
                              aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtct                                                                                                                >  4:35485/1‑146 (MQ=255)
                                aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTa                                                                                                             <  5:138893/147‑1 (MQ=255)
                                   ttGAAAGTGATCTCGCTGGCGGATCTGGTGAAGTACGCCATGGAGCACACGCTGATCTGATTGAGACATACGTGTTCACGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg                                                                                                          <  8:53460/147‑1 (MQ=255)
                                   ttGAAACTGAACTCGCTGGCGGATCTGGTGAAGTAAGCCATGGAGCACAACCTGATCTTATTGCGACATACGGGTGCCCGAAACTTGCAGAAGCCTATGTGGCAGCGGCCATGCCCGCGAACTGGCCTGGCCTGCTGATCTCTATgg                                                                                                          <  6:268000/147‑1 (MQ=255)
                                        cgTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGAGCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAg                                                                                                     <  2:244468/146‑1 (MQ=255)
                                                      cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgcg                                                                                       <  3:35485/147‑1 (MQ=255)
                                                       ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                      <  4:140448/147‑1 (MQ=255)
                                                       ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                      <  6:99644/147‑1 (MQ=255)
                                                            tGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATg                                                                                 <  1:54570/147‑1 (MQ=255)
                                                            tGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATg                                                                                 <  5:208622/147‑1 (MQ=255)
                                                               tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGccc                                                                              <  5:40156/147‑1 (MQ=255)
                                                                            ggAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCGATGGCTGAGCCGGCCTCTTGGCGGGCATGCCCGCTCCCCCAGGtt                                                                 >  7:182095/1‑147 (MQ=255)
                                                                                    aGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGtt                                                         >  8:67553/1‑147 (MQ=255)
                                                                                        gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTGCCACAGGTTTCGTGGTTgggt                                                     >  3:160620/1‑145 (MQ=255)
                                                                                          tctgatTGCCAAATCGGGGTGCCCTAAACTTGCCTAAGCCTATGTGGGAGCGGGCATGCCCGCTAACGGGCCTGGCCTGCTTATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCT‑c                                                   <  4:160620/146‑1 (MQ=38)
                                                                                                     cATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTACCCCCAACCGGCCAGGCCGGCTGATCTATATGGCAGAGCCCGGCTCATAGCCGGCATTCCCCCTCCCCCCGGGTTGGTGGGTGGCTGCGCAAggccta                                         >  8:13310/1‑142 (MQ=255)
                                                                                                                 cGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctgg                                                                                                                           <  7:48724/52‑1 (MQ=255)
                                                                                                                 cGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctgg                                                                                                                           >  8:48724/1‑52 (MQ=255)
                                                                                                                                ccTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCT‑CGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATc            <  7:67553/147‑1 (MQ=255)
                                                                                                                                          aGCGGGCATGCCCGCGCACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCT‑CGCAAGGGCATTGCAATCCCGCCATCCTTGTAGGGCGATCCCTacaaca   >  6:193504/1‑146 (MQ=255)
                                                                                                                                          aGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGGTTCGTGGTTGGCT‑CGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGAGATCCCTAcaacaa  >  6:201977/1‑147 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCT‑CGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499062‑499346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑