Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,735,139 Δ1 bp 7.8% coding (36/3093 nt) PP_3302 → RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,735,1390A. 7.8% 157.7 / 7.4 51coding (36/3093 nt)PP_3302RND family transporter
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base . (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGC  >  NC_002947/3735003‑3735279
                                                                                                                                        |                                                                                                                                             
cACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcgagcga                                                                                                                                  <  8:287309/149‑1 (MQ=255)
cACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcgagc                                                                                                                                    <  1:96173/147‑1 (MQ=255)
cACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcgagc                                                                                                                                    <  7:71620/147‑1 (MQ=255)
 aCGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGat                                                                                                                                 <  6:357934/149‑1 (MQ=255)
                      cACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcgc                                                                                                                                  >  1:291039/1‑126 (MQ=255)
                      cACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcgc                                                                                                                                  <  2:291039/127‑2 (MQ=255)
                       aCTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgt                                                                                                           >  8:263435/1‑149 (MQ=255)
                        cTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgtg                                                                                                          <  3:190425/149‑1 (MQ=255)
                               cgcATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATc                                                                                                    <  4:281568/148‑1 (MQ=255)
                                gcATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGc                                                                                                  >  7:302541/1‑149 (MQ=255)
                                 cATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGcc                                                                                                 >  3:361036/1‑149 (MQ=255)
                                      gctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGc                                                                                            >  5:230125/1‑149 (MQ=255)
                                       ctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTGAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCAGCTCTCGCAGTAC‑GCGAGAGTTGAATCACCCGCTGCCTGATTGTGCTGCTAGCCTTCGcc                                                                                           >  8:356505/1‑149 (MQ=255)
                                       ctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGc                                                                                            <  7:263435/148‑1 (MQ=255)
                                         gcgcGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGAGATCAATCACCCTCTTCCTGATTGAGCTAATCGCCTACGCCgg                                                                                         >  5:66712/1‑149 (MQ=255)
                                             gATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACg                                                                                     >  1:216541/1‑149 (MQ=255)
                                                  cGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGc                                                                                >  3:311702/1‑149 (MQ=255)
                                                   gTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGcc                                                                               >  4:99021/1‑149 (MQ=255)
                                                       aGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCtttt                                                                           <  1:83803/149‑1 (MQ=255)
                                                          tCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcgagc                                                                                                                                    >  8:103195/1‑89 (MQ=255)
                                                          tCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcgagc                                                                                                                                    <  7:103195/89‑1 (MQ=255)
                                                                     gCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGc                                                                                                         >  6:210591/1‑105 (MQ=255)
                                                                     gCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGc                                                                                                         <  5:210591/105‑1 (MQ=255)
                                                                           aCAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCGGCGCTCGCAGTAC‑GCGAGCGATCAATCAcc                                                                                                                         <  1:33765/83‑1 (MQ=255)
                                                                           aCAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCAcc                                                                                                                         >  2:33765/1‑83 (MQ=255)
                                                                           aCAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCagaa                                                       <  3:99021/149‑1 (MQ=255)
                                                                                tCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATcc                                                  >  8:70717/1‑149 (MQ=255)
                                                                                  aGGTCATGGGGCACCAACCATGAGCGAGCTGCGTTTCAACTTATCCGCTCTCGCAGTAC‑GCGAGCGATCAATGACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCATTTTTCAAGCTGGGCCGTGCAGAAGATCCg                                                 <  8:302541/148‑1 (MQ=255)
                                                                                  aGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCg                                                 <  8:334308/148‑1 (MQ=255)
                                                                                   ggTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGAGCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGcc                                               <  6:290307/149‑1 (MQ=255)
                                                                                    gTCATGGGGCACCAACCATGAGCCAGCAGCGTTTCAACTTAACCGCGCTCGCAGTAC‑GCGAGCGATCAATCACACTCTTCCTGATTGTGCTGATCCCCTTCGCCGGCACGCAGGCCTTTTTCAAGCTGCGCCGAGCACAAGATCCGcac                                              >  2:136457/1‑147 (MQ=255)
                                                                                         ggggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCAc                                         >  4:300649/1‑149 (MQ=255)
                                                                                           ggCACCACCCATGCTCCAGCTGCGGTTCAACTTATCCGCGCTCGCATTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGGTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGcc                                               <  4:291168/141‑1 (MQ=255)
                                                                                           ggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGcccagaacg                                        >  3:291168/1‑142 (MQ=255)
                                                                                           ggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGcc                                               <  2:351808/141‑1 (MQ=255)
                                                                                           ggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGcc                                               >  1:351808/1‑141 (MQ=255)
                                                                                           ggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCg                                       >  4:80732/1‑149 (MQ=255)
                                                                                           ggCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCg                                       <  4:361036/149‑1 (MQ=255)
                                                                                                    caTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAg                                  >  8:222411/1‑145 (MQ=255)
                                                                                                            aGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGAtt                      <  3:300649/149‑1 (MQ=255)
                                                                                                                   tttCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATc                   <  5:73685/145‑1 (MQ=255)
                                                                                                                   tttCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATc                   >  6:73685/1‑145 (MQ=255)
                                                                                                                      cAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCt            >  4:105698/1‑149 (MQ=255)
                                                                                                                             tCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCgg     >  4:60818/1‑149 (MQ=255)
                                                                                                                             tCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCgg     >  1:106817/1‑149 (MQ=255)
                                                                                                                             tCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCg      <  3:105698/148‑1 (MQ=255)
                                                                                                                              ccGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGAt                             <  6:98776/124‑1 (MQ=255)
                                                                                                                              ccGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGAt                             >  5:98776/1‑124 (MQ=255)
                                                                                                                               atcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGgc    <  7:63619/146‑1 (MQ=255)
                                                                                                                               atcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGgc    >  8:63619/3‑148 (MQ=255)
                                                                                                                                gcgcTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGgcgc  >  8:50258/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                             
CACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGC  >  NC_002947/3735003‑3735279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑