Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	11,569:1	+G	6.4%	coding (467/1104 nt)	dnaN →	DNA polymerase III subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	11,569	1	.	G	6.4%	308.1 / 12.6	78	coding (467/1104 nt)	dnaN	DNA polymerase III subunit beta
Reads supporting (aligned to +/- strand):  ref base . (38/35);  new base G (2/3);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCCTGTCGACCCTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGAGTTG  >  NC_002947/11422‑11714                                                                                                                                                       |                                                                                                                                                  cccTGTCGACCCTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑c                                                                                                                                                  <  8:345985/149‑1 (MQ=255)      tCGACCCTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCa                                                                                                                                              <  7:229532/148‑1 (MQ=255)        gACCCTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCAtg                                                                                                                                            <  5:188272/148‑1 (MQ=255)          cccTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCAtgctg                                                                                                                                         >  3:262664/1‑149 (MQ=255)            ctgcctgcCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAAGCAGCAtgccgt                                                                                                                                        >  1:101956/1‑146 (MQ=255)             tgcctgccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCtt                                                                                                                                      >  6:19372/1‑149 (MQ=255)               cctgccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGa                                                                                                                                    <  5:19372/149‑1 (MQ=255)                ctgccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAg                                                                                                                                   <  3:155036/149‑1 (MQ=255)                ctgccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAg                                                                                                                                   >  2:92150/1‑149 (MQ=255)                  gccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGt                                                                                                                                 >  2:228402/1‑149 (MQ=255)                   ccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAAGCAGCATGCCG‑TTGAgg                                                                                                                                  <  7:39089/147‑1 (MQ=255)                   ccAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTAGTACCTCAAGCTGCATGCCG‑TTGAgg                                                                                                                                  <  6:252650/147‑1 (MQ=255)                    cAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGttt                                                                                                                               >  3:279700/1‑149 (MQ=255)                     aaTGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTc                                                                                                                              <  4:187553/149‑1 (MQ=255)                     aaTGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTc                                                                                                                              >  5:30515/1‑149 (MQ=255)                             cccGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAAGCAGCATGCCG‑TTGAGGtaatagc                                                                                                                           >  7:325349/1‑138 (MQ=255)                             cccGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAAGCAGCATGCCG‑TTGAGGtaatagc                                                                                                                           <  8:325349/144‑7 (MQ=255)                             cccGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAaca                                                                                                                      >  2:184794/1‑149 (MQ=255)                             cccGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAaca                                                                                                                      >  4:244146/1‑149 (MQ=255)                              ccGTCTGTGGAAGTAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGGCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAaca                                                                                                                      <  3:342161/148‑1 (MQ=255)                                 aCTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGtt                                                                                                                  <  4:27135/149‑1 (MQ=255)                                     tgGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTgcgc                                                                                                              >  1:292026/1‑149 (MQ=255)                                       gaagaaGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTgcgcgc                                                                                                            >  3:104273/1‑149 (MQ=255)                                        aagaagGCCCGGGTTCGCTGACCTGCAACCGGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCt                                                                                                           <  5:196096/149‑1 (MQ=255)                                           aagGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGGTCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTa                                                                                                        >  2:176380/1‑149 (MQ=255)                                              gCCCGGGTTCGCTGACCTGCAACCTGGAGCAGGAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATc                                                                                                      >  1:45305/1‑149 (MQ=255)                                                ccGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCATAGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCACAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCa                                                                                                    >  1:51687/1‑149 (MQ=255)                                                ccGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCAc                                                                                                   <  6:45953/149‑1 (MQ=255)                                                    gTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAg                                                                                                                                   <  2:18750/113‑1 (MQ=255)                                                    gTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAg                                                                                                                                   >  1:18750/1‑113 (MQ=255)                                                     ttCGCTGACCTGCAACATGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACg                                                                                              <  4:305278/149‑1 (MQ=255)                                                        gCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGt                                                                                            >  4:159185/1‑148 (MQ=255)                                                          tGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCAc                                                                                         >  5:141382/1‑149 (MQ=255)                                                            aCCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCAAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCg                                                                                       >  6:152979/1‑149 (MQ=255)                                                              cTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCTTGGCCCAGCAGGATGTGCGCTATTACCTCTA‑CGGCATGCTGCTTGAGGTTTAGCGCAACACGTTGCGCGATGTATCCACCGAGGGTCCCGGcc                                                                                     >  8:297418/1‑149 (MQ=255)                                                              cTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGcc                                                                                     >  1:159920/1‑149 (MQ=255)                                                               tGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCt                                                                                    >  8:171343/1‑149 (MQ=255)                                                               tGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCt                                                                                    >  6:136490/1‑149 (MQ=255)                                                                gCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTg                                                                                   >  5:247567/1‑149 (MQ=255)                                                                     cTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCt                                                                              >  6:183983/1‑149 (MQ=255)                                                                         agca‑gagca‑AGCTGCGCCGCCTGGTCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGc                                                                          <  1:176380/149‑1 (MQ=255)                                                                         agca‑gagca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGc                                                                          >  4:165533/1‑149 (MQ=255)                                                                               agca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTc                                                                                           <  3:199081/127‑1 (MQ=255)                                                                               agca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTc                                                                                           >  4:199081/1‑127 (MQ=255)                                                                                gca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGAt                                                                     <  7:336388/148‑1 (MQ=255)                                                                                 ca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGa                                                                   <  4:262664/149‑1 (MQ=255)                                                                                 ca‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGa                                                                   <  1:184794/149‑1 (MQ=255)                                                                                            cgccTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGAt                                                         <  1:228402/149‑1 (MQ=255)                                                                                                tGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTg                                                                                   <  8:124782/119‑1 (MQ=255)                                                                                                tGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTg                                                                                   >  7:124782/1‑119 (MQ=255)                                                                                                 gATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGc                                                    <  3:165533/149‑1 (MQ=255)                                                                                                  aTCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAg                                                     >  6:178977/1‑147 (MQ=255)                                                                                                  aTCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAg                                                     <  3:244146/147‑1 (MQ=255)                                                                                                  aTCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGc                                                    <  8:194687/148‑1 (MQ=255)                                                                                                     gAGCGAACCAGCTTCGACATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATCGGCGCGCTCATCGAGCaaa                                                 <  6:30515/148‑3 (MQ=255)                                                                                                     caGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCagg                                                 <  5:152979/147‑1 (MQ=255)                                                                                                      aGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCaggag                                               >  6:98131/1‑149 (MQ=255)                                                                                                          aaCCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATc                                           <  7:297418/149‑1 (MQ=255)                                                                                                           aCCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCg                                          >  8:254729/1‑149 (MQ=255)                                                                                                               gCTTCGCCATGGCCCAGCAGGATGAGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGacac                                      >  2:156474/1‑149 (MQ=255)                                                                                                                cTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGacac                                      >  2:295036/1‑148 (MQ=255)                                                                                                                cTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACAcc                                     <  3:370351/149‑1 (MQ=255)                                                                                                                 ttCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGacac                                      >  6:202908/1‑147 (MQ=255)                                                                                                                 ttCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGacac                                      >  2:136398/1‑147 (MQ=255)                                                                                                                 ttCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGacac                                      <  5:202908/147‑1 (MQ=255)                                                                                                                    gCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAgg                                  <  1:92150/148‑1 (MQ=255)                                                                                                                             cagcagGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTGGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGcc                        <  6:73274/149‑1 (MQ=255)                                                                                                                                    aTGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTaaa                 <  3:55526/149‑1 (MQ=255)                                                                                                                                    aTGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTaaa                 <  5:136490/149‑1 (MQ=255)                                                                                                                                     tgtgCGCTATTACCACAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTaaa                 <  2:51687/148‑1 (MQ=255)                                                                                                                                      gtgCGCTATTACCTCAA‑CGGCATGCTCCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAgg               >  4:7215/1‑149 (MQ=255)                                                                                                                                       tgCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAgg               >  7:118603/1‑148 (MQ=255)                                                                                                                                       tgCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGt              <  7:171343/149‑1 (MQ=255)                                                                                                                                       tgCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGt              >  5:107930/1‑149 (MQ=255)                                                                                                                                              ttACCTCAA‑GGGCATGCTGCTTGAGGTTTACCGCAACTCGTTGCGCGCTGTATCCTCCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTg        <  4:104273/148‑1 (MQ=255)                                                                                                                                              ttACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCt         <  3:159185/147‑1 (MQ=255)                                                                                                                                                aCCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGa      <  5:178977/148‑1 (MQ=255)                                                                                                                                                  cTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGAGtt   >  1:281631/1‑149 (MQ=255)                                                                                                                                                  cTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGAGtt   >  5:75058/1‑149 (MQ=255)                                                                                                                                                     aa‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGAGTTg  <  2:292026/147‑1 (MQ=255)                                                                                                                                                      a‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAgg                                  >  8:126123/1‑114 (MQ=255)                                                                                                                                                       |                                                                                                                                                  CCCTGTCGACCCTGCCTGCCAATGACTTCCCGACTGTGGAAGAAGGCCCGGGTTCGCTGACCTGCAACCTGGAGCA‑GAGCA‑AGCTGCGCCGCCTGATCGAGCGAACCAGCTTCGCCATGGCCCAGCAGGATGTGCGCTATTACCTCAA‑CGGCATGCTGCTTGAGGTTTCCCGCAACACGTTGCGCGCTGTATCCACCGACGGTCACCGCCTGGCGCTTTGCTCGATGAGCGCGCCGATCGAGCAGGAGGATCGACACCAGGTCATCGTGCCGCGTAAAGGTATCCTGGAGTTG  >  NC_002947/11422‑11714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑