Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,126,645:1 +C 100% intergenic (‑99/+50) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,126,6451.C95.9% 158.3 / ‑0.1 49intergenic (‑99/+50)tdcG‑II/gcvP‑IL‑serine dehydratase/glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (20/27);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑C‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAG  >  NC_002947/1126500‑1126785
                                                                                                                                                  |                                                                                                                                              
gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑c                                                                                                                                               <  3:96989/147‑1 (MQ=255)
gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCCAATGCGGAGGTATAGCCACCATCCCATTCGCGGGTAAA‑cac                                                                                                                                             >  6:377739/1‑147 (MQ=255)
  ggggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑c‑ccgc                                                                                                                                          <  6:221430/149‑4 (MQ=255)
                    gAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGccc                                                                                                                           <  5:15482/146‑1 (MQ=255)
                    gAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGc                                                                                                                         <  3:112437/148‑1 (MQ=255)
                          gTCGACGACTCTCAGTGACATGTGCACCGCCTGCCAGGCTGTTGGGTGGACATTGAGCCCTCTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCaa                                                                                                                  <  8:349403/149‑1 (MQ=37)
                          gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGATTGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCaa                                                                                                                  <  6:88509/149‑1 (MQ=255)
                          ctccatcaCGCTCAGTCACCTGTCGCCCTCCTGCCTGGGTGTTGGGTGGACATTGCGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCaa                                                                                                                  <  8:344604/142‑1 (MQ=25)
                                   gCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGACCCCTGTAGGCGCGGGTTTAACCCCCAATGCGGAGGTATAGCCACCAACGCATTAGCGGGTAAACC‑C‑CCTCCTACACAGAGCCCGCCACCCAACCCAGGTgg                                                                                                         >  4:61364/1‑149 (MQ=255)
                                         tGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGACCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa                                                                                                   <  3:61364/149‑1 (MQ=255)
                                               gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGCATGCGGAGGTATAGCCACCATCGCATTAGCGGGTAAACC‑C‑GCGACTACACCGAGCCCCCCCCCCACCCCACGGGGCGGGGCGGGCTa                                                                                             >  8:258124/1‑149 (MQ=255)
                                                tgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt                                                                                            <  3:293354/149‑1 (MQ=255)
                                                  gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAAAGCGGAGGTTTAGCCCCCATCGCCTTAGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCCCCCCAGCCAGGt                                                                                                           >  6:72726/1‑132 (MQ=37)
                                                  gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGt                                                                                                           <  5:72726/132‑1 (MQ=255)
                                                   aCCTCCGGCCTGGTTGTGGGGGGGACATTGCGCCCTGTAGGCGCGGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg                                                                                         <  5:6173/149‑1 (MQ=37)
                                                     ctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTTTAGCCACCATGGCATTAGCGGGTAAACC‑C‑GCAACTACACAGAGCCCGCCCCCCAACCCAGGTGGCGGGGAGGGCTATAAGGc                                                                                       >  1:120433/1‑149 (MQ=255)
                                                     cgcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc                                                                                       <  1:318271/147‑1 (MQ=255)
                                                     ccccggCCCGGTGGTTGGGGGGACTTTGCGCCCTGTCGGGGCGGGTTTACCCGCTAATGCGGCGGTATACCCACCATCGCATTCGGGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc                                                                                       <  8:129290/144‑1 (MQ=25)
                                                       cctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCCAATGCGGGGGGATAGCCACCATCGCATTAGCCGGTAAACC‑C‑GCGCCAACACACAGCCCCCCACCCAAGCCAGGGGGCGGGGGGGGGTATCATGCgg                                                                                     >  8:79901/1‑148 (MQ=255)
                                                        ctGCCAGGGTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTCCCCGCGAATGCGGAGGTCTAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                    <  5:316430/149‑1 (MQ=38)
                                                           ccAGGTTGTGGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc                                                                                   <  3:57537/147‑1 (MQ=255)
                                                            cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa                                                                                <  5:301705/149‑1 (MQ=255)
                                                               gttgttGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGAGCTATCAGGACGCCTGATAg                                                                             <  2:131608/149‑1 (MQ=37)
                                                               gttgttGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATGCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAg                                                                             <  7:150528/149‑1 (MQ=255)
                                                                  gttgGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CAC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGct                                                                           <  6:113999/148‑1 (MQ=255)
                                                                          gACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatcga                                                                  <  6:144140/149‑1 (MQ=255)
                                                                                 aGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAg                                                           <  5:377739/149‑1 (MQ=255)
                                                                                             gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                    >  8:268081/1‑112 (MQ=255)
                                                                                             gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                    <  7:268081/112‑1 (MQ=255)
                                                                                                       ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                     >  1:148380/1‑149 (MQ=255)
                                                                                                        cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc                                    <  7:258124/149‑1 (MQ=255)
                                                                                                         gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTaca                                                                                                                                   >  4:355667/1‑53 (MQ=18)
                                                                                                         gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTaca                                                                                                                                   <  3:355667/53‑1 (MQ=18)
                                                                                                         gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc                                    >  6:319771/1‑148 (MQ=255)
                                                                                                            aaTGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGaa                                 >  6:284791/1‑148 (MQ=255)
                                                                                                               gCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCTCACACCAGGTTGCGGTCGCCGAACACg                             >  7:298472/1‑149 (MQ=255)
                                                                                                                cGGAGGTATAGCCACCAACGCATTCGCGGGTAAA‑C‑CAGCTACTACACAGAGCCCGCCACCCAAGCCACGTTGCGCGGAGCGCTATCAGGCGTCCTGATAGCTCTCGATCTACCGGCACGCGCACACCAGGTTGCGGTCGCCGAACACga                            >  1:54236/1‑148 (MQ=255)
                                                                                                                 ggAGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt                           >  2:297521/1‑149 (MQ=255)
                                                                                                                   aGGTATAGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGt                         <  7:79901/149‑1 (MQ=255)
                                                                                                                        taGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGCTGCGGTCGCCGAACACGTTGTCGAcc                    <  2:120433/149‑1 (MQ=255)
                                                                                                                         aGCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc                   >  6:25592/1‑149 (MQ=255)
                                                                                                                          gCCACCATCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATTGCTCTCGATCGACGGGGAGGCGCACACCAGGTTGCGGTCGGCGATCACGTTGTCGACCCg                  >  8:156493/1‑149 (MQ=255)
                                                                                                                            caccaTCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc                >  1:150000/1‑149 (MQ=255)
                                                                                                                             accaTCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctct                                                                         >  5:97507/1‑91 (MQ=255)
                                                                                                                             accaTCGCATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctct                                                                         <  6:97507/91‑1 (MQ=255)
                                                                                                                              ccaTCCAATTCGCGGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCg              <  7:224354/149‑1 (MQ=255)
                                                                                                                                aTCGCATTCGCGGATAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc            >  8:61511/1‑149 (MQ=255)
                                                                                                                                         gcgGGTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc     >  7:136246/1‑147 (MQ=255)
                                                                                                                                         gcgGGTAAACC‑C‑GCGCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa   <  6:173899/149‑1 (MQ=255)
                                                                                                                                           gggTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg  >  2:278084/1‑148 (MQ=255)
                                                                                                                                             gTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg                                                                                         >  4:132719/1‑59 (MQ=38)
                                                                                                                                             gTAAACC‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg                                                                                         <  3:132719/59‑1 (MQ=38)
                                                                                                                                                  |                                                                                                                                              
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑C‑C‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAG  >  NC_002947/1126500‑1126785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑