Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,392,921:1	+A	13.5%	intergenic (+91/‑95)	PP_1214 → / → ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,392,921	1	.	A	13.5%	132.7 / 15.7	37	intergenic (+91/‑95)	PP_1214/ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base . (12/20);  new base A (2/3);  total (14/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTG  >  NC_002947/1392775‑1393069                                                                                                                                                    |                                                                                                                                                      aGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑a                                                                                                                                                    <  7:25074/149‑1 (MQ=255)    aCGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑Attt                                                                                                                                                 <  8:348338/149‑1 (MQ=255)         tATTCCAATGCCCAGATTTCCGTCGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAAt                                                                                                                                            >  7:354031/1‑149 (MQ=255)                aTGCCGAGATTTCCGACGAGATCATGGAAAAGCTCGGCTAAGGTGTTTTGTCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGTTATTAGTCG‑C‑ATTTCAAATgtgttg                                                                                                                                      <  4:311613/148‑1 (MQ=38)                     cAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTAc                                                                                                                                <  1:160694/149‑1 (MQ=255)                     cAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTAc                                                                                                                                >  1:291745/1‑149 (MQ=255)                       gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCt                                                                                                                              <  3:268756/149‑1 (MQ=255)                       gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCt                                                                                                                              <  2:291745/149‑1 (MQ=255)                        aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTg                                                                                                                             <  2:18241/149‑1 (MQ=255)                            ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                          <  6:28078/148‑1 (MQ=255)                            ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCAC‑ATTTGAAATG‑GGCGTAATACCTGTAg                                                                                                                          <  1:138792/148‑1 (MQ=18)                              gacgaTATCATGCAAAACCTCGGCTAAGCTGATTTGGCATATTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCAC‑ATTTGAAATG‑GGCGTAATACCTGTAGGAg                                                                                                                       <  7:351568/149‑1 (MQ=17)                               acgaGATCATGGAAAACCTCGGCTAAGGTTATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                      <  8:216061/149‑1 (MQ=255)                                 gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                    <  1:51707/149‑1 (MQ=255)                                    aTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGtt                                                                                                                 <  2:174341/149‑1 (MQ=255)                                     tCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGtt                                                                                                                 <  2:214317/148‑1 (MQ=255)                                       aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAAtg                                                                                                                                           >  2:374722/1‑120 (MQ=255)                                       aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAAtg                                                                                                                                           <  1:374722/120‑1 (MQ=255)                                          gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAccc                                                                                                            <  8:354031/148‑1 (MQ=255)                                                                                        gTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                    <  1:46051/95‑1 (MQ=255)                                                                                        gTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                    >  2:46051/1‑94 (MQ=255)                                                                                                                        gTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAcccc                             >  1:353202/1‑149 (MQ=255)                                                                                                                              ccGCTCCTACAGGTATTACGCC‑A‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                       >  3:149741/1‑149 (MQ=255)                                                                                                                              ccGCTCCTACAGGTACTGCACCAC‑ATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc                              >  3:40489/1‑142 (MQ=17)                                                                                                                              ccGCTCCTACAGGTACTGCACCAC‑ATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc                              <  4:40489/142‑1 (MQ=17)                                                                                                                              ccGCTCCTACAGGTACTGCACCAC‑ATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTA‑CCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                      >  2:45213/1‑149 (MQ=18)                                                                                                                                    cTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcgagat                 >  7:32739/1‑145 (MQ=255)                                                                                                                                    cTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                      <  8:32739/144‑1 (MQ=255)                                                                                                                                      aCAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGAct                                                  <  6:379066/114‑1 (MQ=255)                                                                                                                                      aCAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGAct                                                  >  5:379066/1‑114 (MQ=255)                                                                                                                                       cAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                         >  2:309655/1‑122 (MQ=255)                                                                                                                                       cAGGTATTACGCC‑C‑ATTTCAAATGTGCTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                         <  1:309655/122‑1 (MQ=255)                                                                                                                                          gTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc           >  8:18797/1‑149 (MQ=255)                                                                                                                                           tATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc           >  1:195704/1‑148 (MQ=255)                                                                                                                                               aCGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATg       <  7:18797/148‑1 (MQ=255)                                                                                                                                                cGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATgg      <  2:195704/148‑1 (MQ=255)                                                                                                                                                 gCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCg    >  8:175651/1‑149 (MQ=255)                                                                                                                                                  cc‑c‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt   >  7:176846/1‑149 (MQ=255)                                                                                                                                                   c‑c‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg  >  7:319905/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑C‑ATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTG  >  NC_002947/1392775‑1393069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑