| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 94.9% | 123.1 / 0.2 | 39 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (23/14); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑CCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932495‑1932780 | ggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCTCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑cccgc < 2:36259/149‑4 (MQ=255) ttCGAAGAGGATCTGCACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTTTAGGAGC‑GGGCTTGCCCGC‑GAAggg > 8:182907/1‑149 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCAGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGc > 7:3593/1‑147 (MQ=255) ttCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAAt > 6:464/1‑149 (MQ=255) cATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcg > 4:150244/1‑149 (MQ=255) gTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 3:150244/149‑1 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 7:182907/149‑1 (MQ=255) cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAAATTTCGCCTACGCGGATCAACGAt > 7:204274/1‑149 (MQ=37) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGGTc > 1:147904/1‑149 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg > 2:72179/1‑149 (MQ=255) ccgTAACCGCTGGGGCGGCTTGGCGGCCCGGCGCGGGCAAGCCCGCGCCTACACAGGATATGCGTCCCCTGTCGGAGC‑GGGCTGGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg < 3:229801/147‑1 (MQ=37) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGAAAAAAGATATGCGGGCCCGGTTGGAGCGGGGGTTG‑CCCC‑GAAAGGCGGGAAAGCAGCCCCCAATTTTAGACTAACCGGGTAAGCGATACCGCACGGGGAt > 4:143431/1‑149 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg > 7:30242/1‑149 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAACGGATATCCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 5:113617/149‑1 (MQ=255) gccaccccTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGGCCCGTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 1:131521/137‑1 (MQ=37) gCCCTTCGCGGGCAAGCCCGCTCCTACAATGTATATGCGTCCCCTTTAGGAGG‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcagc < 2:147904/149‑1 (MQ=38) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt > 7:91815/1‑148 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGCGGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt > 1:98725/1‑149 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 5:464/149‑1 (MQ=255) aGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 1:72179/149‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc > 1:212770/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 2:4469/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 6:138342/1‑149 (MQ=255) tCCTACATATGAAATGCTTCCCGTGTTGGTGCGGGGTTTC‑CCGC‑GAAGGGCTGCAAGGCAGCCCCATATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa < 8:204274/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc > 5:308024/1‑113 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 6:308024/113‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 4:222874/1‑149 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 1:342222/1‑149 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 2:73439/1‑149 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 2:224076/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 7:197423/1‑149 (MQ=255) cGTCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 5:79675/1‑149 (MQ=255) tCCCCTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 3:222874/149‑1 (MQ=255) cccTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg < 8:30242/149‑1 (MQ=255) tttGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCCGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 2:327481/3‑149 (MQ=255) ccTGTAGGAGC‑GGGCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 3:117657/1‑149 (MQ=255) tAGGAGC‑GGGCTTGCCCGC‑GAAGGGCCGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATgc < 1:327481/148‑2 (MQ=255) gggCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat < 1:4469/149‑1 (MQ=255) gggCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat < 5:138342/149‑1 (MQ=255) ggCTTGCCCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAGATCATCGTTGCCGATGTTCACGTAtt > 2:145613/1‑149 (MQ=255) | GGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTTG‑CCGC‑GAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932495‑1932780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |