| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,271,674:1 | +T | 9.6% | intergenic (+58/‑42) | PP_t32 → / → PP_t33 | tRNA‑Asp/tRNA‑Asp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,271,674 | 1 | . | T | 9.6% | 189.3 / 16.7 | 52 | intergenic (+58/‑42) | PP_t32/PP_t33 | tRNA‑Asp/tRNA‑Asp |
| Reads supporting (aligned to +/- strand): ref base . (23/24); new base T (3/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGAACACCTT > NC_002947/2271527‑2271821 | agGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgt < 5:230138/149‑2 (MQ=255) cGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGa < 1:233835/149‑1 (MQ=255) cACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATc > 4:325292/1‑149 (MQ=255) aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCATTAACAGATGGGTGCGTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATc > 1:2869/1‑148 (MQ=255) tGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg‑gg > 8:47899/1‑140 (MQ=255) tGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg‑gg > 8:41886/1‑140 (MQ=255) tGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg‑gg < 7:47899/140‑1 (MQ=255) tGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg‑gg < 7:41886/140‑1 (MQ=255) cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGGCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGt < 4:122902/149‑1 (MQ=255) cAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTGCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGttt < 7:94030/149‑1 (MQ=255) aGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTc < 2:90158/149‑1 (MQ=255) gTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCgg < 3:325292/148‑1 (MQ=255) tCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGgc < 6:75075/148‑1 (MQ=255) gTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCt > 5:255240/1‑149 (MQ=255) cTGGCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGc < 6:312999/149‑1 (MQ=255) tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCg < 6:255240/147‑1 (MQ=255) aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTa < 3:334411/148‑1 (MQ=255) gCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGtt < 5:256689/149‑1 (MQ=255) tCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCg < 7:308388/135‑1 (MQ=255) tCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCg > 8:308388/1‑135 (MQ=255) gcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCATTAAAAGATCCTTGCAAACCAACCGTCTAAAACCACAAAACACATCCCTTT‑ATCTGATAAAATTTTCAATCCGTCACAAGCATGAACGATACGCAGCGGTAGTTCAATCGGTTa > 6:137753/1‑149 (MQ=255) gAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATa < 3:172186/118‑1 (MQ=255) gAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATa > 4:172186/1‑118 (MQ=255) cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGt > 2:332439/1‑149 (MQ=255) gcgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg > 3:347392/1‑149 (MQ=255) cgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg‑g < 4:347392/149‑1 (MQ=255) cgcCATTCTTCAGTATCACCTGGGCGCCTGGCACCTGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCATTCGGGCTGAATCCTGATCTATTCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg‑g < 6:135979/149‑1 (MQ=25) cATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGt > 5:215784/1‑149 (MQ=255) ttttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACAGGCCTGTCACGCCGG‑GGGTcgc > 1:228514/1‑149 (MQ=255) cgcaaCAGATCGGTGCCTGGCACCGGGCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTa < 6:348940/112‑1 (MQ=25) cAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGt > 1:281581/1‑149 (MQ=255) cAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGt > 1:286440/1‑149 (MQ=255) aTGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTccc > 2:174392/1‑149 (MQ=255) gggTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGt > 5:100718/1‑149 (MQ=255) ggTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTc < 1:332439/149‑1 (MQ=255) cGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCgg < 6:255250/101‑1 (MQ=255) aGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc < 2:281581/149‑1 (MQ=255) aGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc < 2:286440/149‑1 (MQ=255) agcccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGttt > 3:151036/5‑148 (MQ=14) gcccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGtt < 5:260048/114‑1 (MQ=17) gcccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGtt > 6:260048/4‑117 (MQ=17) ccACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGCGGGTCGCGGGTTCGAGTCCCGTCCGCTgcg < 1:225757/135‑1 (MQ=255) ccACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTgcg > 2:225757/1‑134 (MQ=255) cccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc > 6:144136/3‑147 (MQ=255) cacaAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAg > 5:53560/1‑149 (MQ=255) acaAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg > 3:46372/1‑149 (MQ=255) acaAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg > 4:212397/1‑149 (MQ=255) ggcatgtGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTcc < 4:151036/143‑1 (MQ=255) ggCACATTCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACTATACGCTGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTccc < 2:2869/149‑1 (MQ=255) acaTGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTccc > 2:233904/1‑146 (MQ=255) acaTGCCTTG‑CGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTAAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGCGTACGAGTCCCGTCCGCTGCGCCATCTTCGTATCAAGGTccc > 6:250803/1‑146 (MQ=17) ccTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGAacac < 6:53560/149‑1 (MQ=255) cTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGGCTGAACGATACGGAGCGGTAGTTCAGTGGTTTAGAATACGGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCGGTCCGCTGCGCCATCCTCGTTTCAAGGTCCCTAGAACAcc < 5:250803/149‑1 (MQ=17) cTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGa > 6:232842/1‑104 (MQ=255) cTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGa < 5:232842/104‑1 (MQ=255) ttG‑GGCTGATCCCAGTTTCAATCGAGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGAACACCt > 1:230300/1‑149 (MQ=255) tG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGAACACCtt > 7:301660/1‑149 (MQ=255) | AGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGG‑GGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGAACACCTT > NC_002947/2271527‑2271821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |