| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 89.4% | 135.3 / 10.6 | 47 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base A (20/22); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑T‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCC > NC_002947/278598‑278876 | tCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑T‑aaac > 2:218879/1‑147 (MQ=255) gCTACAGCTGATCGCGACATACGTGTGCTGGTCCAGTGGTCCGGTTTTTTTACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑T‑AA‑accc < 1:127044/149‑2 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTc < 5:228864/149‑1 (MQ=255) gATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCtactac > 5:235775/1‑148 (MQ=255) aTCGCGACAGACGTGTGCTTGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTacac < 8:160097/149‑1 (MQ=255) cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACAt < 7:184268/149‑1 (MQ=255) gACGTGTGATGGTCAGGTGGTCCGGTATATATAAACTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACCCACATGCCt > 8:41715/1‑148 (MQ=255) ggtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGa < 4:33235/148‑1 (MQ=255) tgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGAt < 7:168070/148‑1 (MQ=255) gCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGAtat > 4:17692/1‑147 (MQ=255) tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑ACCCCTCCTACACACATGCATCAAGATCtgt > 1:358009/1‑149 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCTC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCtgtg > 3:225773/1‑148 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑ACCGCTCCTAAACAAATGCCTAAAGaaatgat > 3:19942/1‑142 (MQ=255) tccggtgttccggtATATATTCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATTTTTATAGAAGGGGTATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTAAACACATGCCTCAATATCTGTGc < 6:122380/148‑1 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 7:295013/149‑1 (MQ=255) gtccggtATATATACACTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg < 3:199988/148‑1 (MQ=255) tccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg > 7:105753/1‑147 (MQ=255) cggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGc < 8:46586/149‑1 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 1:218879/149‑1 (MQ=255) tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTc < 4:129488/105‑1 (MQ=255) tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTc > 3:129488/1‑105 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg > 2:369141/1‑138 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg < 1:369141/138‑1 (MQ=255) tttATAAAATTTTTATTAAATAACTTTTTGTATTATTTTTTGAAGGGGGATTTGTTACTGAGGTGCCGGGCTCTTCGG‑GGT‑TAAA‑CCCGCTCCTACACACATGCCTCAAGTTCTGTGCACTGTAGGAGGGGTTTTACCCGCGAAAggg < 4:225773/148‑1 (MQ=255) tttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGTCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGc > 8:325859/1‑149 (MQ=255) ttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGc < 6:375294/148‑1 (MQ=255) tataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGTCGGGCTCTTCGC‑GGG‑T‑AACCCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGcc < 7:325859/148‑1 (MQ=255) tataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATTCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCg > 1:340431/1‑149 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt < 8:129476/119‑1 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt > 7:129476/1‑119 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑ACCGCTCCTACACACATGCCTCAAGATATGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCgg > 5:357806/1‑148 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAg < 5:92502/149‑1 (MQ=255) aactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg > 6:99478/1‑149 (MQ=255) actTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTa > 6:284739/1‑73 (MQ=255) actTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTa < 5:284739/73‑1 (MQ=255) tttttGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCGCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg > 8:861/1‑149 (MQ=255) aaTATTTATAGAAGGGGGATTTGTTACACCGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAACATCTGTGCACTGTAGGCGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTccc < 7:178242/149‑1 (MQ=39) tttttATAGACGGGGTATTTGTTACAGAGGTGCCGGGCTCTGCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAACATCTGTGCACTGTAGCAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGa < 8:105753/147‑1 (MQ=255) tatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTAAACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTTACCGCGAAAGGGCCGGAACAGGTTTACACGGAGGCCCCAGTccc > 2:128076/1‑147 (MQ=255) taGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATATGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACt > 5:137580/1‑149 (MQ=255) aGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACtt > 8:101657/1‑149 (MQ=255) gAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTg < 5:99478/149‑1 (MQ=255) cAGAGGTGCCGGGCTCTTCGCTGTA‑A‑AA‑CCCGCTCATACACACATGCATCATTATCTGTCCACTGTAGGAGGGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgc < 8:308332/148‑1 (MQ=255) cAGAGGTGCCGGGCTCTTCGC‑GGGAA‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgcg > 4:284785/1‑149 (MQ=255) gCCGGGCTCTTCGG‑GGG‑TAAA‑GCGGTTCTTAAACACATGCTTTTTTTTCTGTGATCTGTAGGTGCGGTTTTTCCCGCGAAAGGGCCGTAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACtctc < 2:358009/149‑1 (MQ=255) ccGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctc > 6:100889/1‑148 (MQ=255) ccGGGCTCTTCGC‑GGG‑TAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctc < 5:100889/148‑1 (MQ=255) ggCTCTTCGC‑GGG‑A‑AACCCCGCTCCTACACACATGCCTCACGAGCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttcc < 7:41715/149‑1 (MQ=39) | TCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGC‑GGG‑T‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCC > NC_002947/278598‑278876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |