| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,773,941:1 | +G | 13.2% | coding (945/1053 nt) | calA → | coniferyl alcohol dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,773,941 | 1 | . | G | 13.2% | 122.8 / 17.3 | 38 | coding (945/1053 nt) | calA | coniferyl alcohol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (18/15); new base G (2/3); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
TCGGCCTGATCGAACCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCTGTACGGGCCTCTTCGCGG > NC_002947/2773799‑2774081 | tCGGCCTGATCGAACCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATc < 1:68847/149‑1 (MQ=255) cgtccTGATCGAACCGATCGACCCGGCTTTGCATGCGGGCAATCTGGGGATGCAGGGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTGAGGATGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCg < 8:331569/146‑1 (MQ=255) tGATCGAACCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGCGGATATCcagca > 5:252570/1‑147 (MQ=255) gAACCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTgg < 4:175443/148‑1 (MQ=255) aCCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTCGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGCACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGATGGata > 2:326248/1‑149 (MQ=255) gATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATcc > 7:159571/1‑149 (MQ=255) aCCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCT‑GCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGCGGATATCCAGCATCTCGATAT‑CGCAAcg > 8:295364/1‑148 (MQ=255) ggCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCT‑GCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGCGGATATCCAGCATCTCGATAT‑CGCAAcggatg < 7:295364/149‑6 (MQ=37) gCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGCGGATATCCAGCATCTCGATAT‑CGCAAcggatg < 8:337990/149‑6 (MQ=38) cATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAAc < 7:350275/149‑1 (MQ=255) aTTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGCGGATATCCAGCATCTCGATAT‑CGCAAcggatgtc < 6:252570/149‑8 (MQ=37) aTTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAAc > 6:329858/1‑148 (MQ=255) cATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAgg < 6:188028/148‑1 (MQ=255) cATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGc < 3:84900/149‑1 (MQ=255) gCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTa > 6:187848/1‑149 (MQ=255) cGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTAt > 5:14392/1‑149 (MQ=255) cAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑Ga < 7:99755/105‑1 (MQ=255) cAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑Ga > 8:99755/1‑105 (MQ=255) gTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGgatga < 8:159571/148‑1 (MQ=255) gaAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGCCTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCg > 2:328026/1‑149 (MQ=255) gaAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCg > 3:327655/1‑149 (MQ=255) aaGCGCCGGGTGCTGGCGGGGTCGTTGATAGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGAGCCGAGCACGACATCCGTTGC‑GAAATCGAGATGCTGGATATCCGCAACATCAACAAGGCCTATGAGAGGATGATTGcctg > 2:305897/1‑147 (MQ=255) gacGGGTGCTTGCGGGGTCTTTGATGGGGGGGATTGCCGAGACTCATGAAGTGCTGGACTTCTGCGCCGAGCATGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGAt < 1:305897/147‑1 (MQ=255) gCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTAc < 3:156086/147‑1 (MQ=255) tCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGa > 6:147252/1‑148 (MQ=255) tCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGa > 6:145171/1‑148 (MQ=255) ttGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACg < 6:14392/149‑1 (MQ=255) tCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCt > 4:142393/1‑149 (MQ=255) ggAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCTGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCtgat < 1:326248/149‑1 (MQ=255) ggAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCtgat > 1:280462/1‑149 (MQ=255) gCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCg > 2:90038/1‑149 (MQ=255) tGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGt > 2:95905/1‑149 (MQ=255) ttCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCt > 4:190337/1‑149 (MQ=255) gcCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCTGTACgg < 5:329858/149‑1 (MQ=255) aCGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCTGTACGGGCCTCtt > 8:212966/1‑149 (MQ=255) aTCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCTGTACGGGCCTCTTCGCgg < 5:187848/149‑1 (MQ=255) ccGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATgg > 5:72930/1‑98 (MQ=255) ccGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATgg < 6:72930/98‑1 (MQ=255) | TCGGCCTGATCGAACCGATCGACCCGGCATTGCATGCGGCCAATCTGGTGATGAAGCGCCGGGTGCTGGCGGGGTCGTTGATCGGGGGGATTGCCGAGACTCAGGAAGTGCTGGACTTCTGCGCCGAGCACGACATCCGTTGC‑GATATCGAGATGCTGGATATCCGCAACATCAACCAGGCCTATGAGCGGATGATTGCCGGTGATGTGAAGTACCGGTTCGTGATCGACATGGCCACGCTACAGGGCTGATTGATCGGTTGCCTGTACGGGCCTCTTCGCGG > NC_002947/2773799‑2774081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |