| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 95.6% | 143.3 / ‑0.0 | 45 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (24/19); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA > NC_002947/3845557‑3845840 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gg > 7:4564/1‑149 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gggc < 3:361885/149‑3 (MQ=255) ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCAGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gggctg < 5:224777/149‑5 (MQ=255) gTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGAGTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGAt > 1:274772/1‑149 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGAt < 5:106647/148‑1 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATc < 8:4564/149‑1 (MQ=255) gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGt > 1:111801/1‑148 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGATGGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGt > 1:303011/1‑148 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAg > 6:177183/1‑149 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAg > 2:45712/1‑149 (MQ=255) aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGc < 5:254163/148‑1 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCAc < 4:9301/147‑1 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGa > 3:71781/1‑149 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGAt < 4:71781/149‑1 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTCTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGa < 5:318440/148‑1 (MQ=255) aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATg > 2:100655/1‑149 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGa < 6:246624/149‑1 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATc < 1:45712/147‑1 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATc < 5:177183/147‑1 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGcc > 6:106150/1‑149 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATg > 7:277944/1‑140 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATg > 7:283135/1‑140 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATg < 8:277944/140‑1 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATg < 8:283135/140‑1 (MQ=255) gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCcgc < 6:338211/149‑1 (MQ=255) gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTg > 5:170052/1‑149 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGc > 6:66065/1‑149 (MQ=255) ggcGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGCCTAAGCGCTGGATGACGGGCAGAAAAGCCGTCTTTC‑GCGGG‑CTGATCCATCGAGAGACACGATCGATCAGCCAGCTCCCTGACAGGc > 8:119397/3‑149 (MQ=255) aCTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt < 5:106150/149‑1 (MQ=255) cATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCAc > 1:24338/1‑149 (MQ=255) ttgttattgttGTCATCG‑‑CTGTCTCTTTGTGTGAAGGGTTGGGCAATACTATGAGGTGTATTAGGGGCTCAAAAGCCGTGTTTCGGG‑GG‑CTTATCCTTCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagc < 2:24338/148‑1 (MQ=255) tttgttgTAATCG‑‑CGGTCTGTCTTGCTTAAGCGTTCCGCAAGATTATGAGTTGTATTAGGGGCTGAAACCCGGTTTTGC‑GG‑GGCCGGATCCAACGATCCCCACTTTGTATCTGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg < 2:303011/147‑1 (MQ=255) gttgtCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtgg > 3:191423/1‑149 (MQ=255) gTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCacactg > 1:325012/1‑144 (MQ=255) tctctcTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc < 6:170052/149‑1 (MQ=255) tctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc > 6:265666/1‑147 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc < 5:379212/148‑1 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 8:103399/1‑148 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt < 1:100655/149‑1 (MQ=255) ggATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCTTCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 5:78077/1‑148 (MQ=255) gATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCCCGCCCTGCCAGGCCGGTGCAAGCCGCAACAGGTGGTCAAGGAGGCGCCGAGCCGCCAGCGAGAGCGTGTGCCGGTCGCGCCTg > 4:146629/1‑147 (MQ=255) gACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCCCCGAGCCGACAGCGAGACCGTGTGCCGGTCGCGCATGCAGAt > 5:253862/1‑149 (MQ=255) aCGGGAATAAATGCCGTGTTGC‑GCGGG‑CTGATCCATGGTCAGCCACTTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt < 2:325012/148‑1 (MQ=255) ggCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGCCAGCGAGAGCGGGTGCCGGTCGCGCATGCAGATGAc > 3:33529/1‑148 (MQ=255) gAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt > 6:230390/1‑148 (MQ=255) aaaaGACGTGTTTC‑GCGGG‑CTGATCCCTCGTCAGCCCCGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCCCCGAGCCGCCAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt > 3:301485/1‑148 (MQ=255) aaaaGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc < 7:103399/149‑1 (MQ=255) aCGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCaa < 2:274772/149‑1 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCG‑‑CGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA > NC_002947/3845557‑3845840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |