| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,984,043 | A→C | 10.2% | intergenic (+37/+153) | ilvE → / ← PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,984,043 | 0 | A | C | 10.2% | 167.2 / 9.9 | 59 | intergenic (+37/+153) | ilvE/PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (28/25); new base C (3/3); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
CACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTGA > NC_002947/3983896‑3984190 | cACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAAt > 7:65848/1‑149 (MQ=255)cACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAAt > 5:232696/1‑149 (MQ=255) ccTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCAt < 6:232696/149‑1 (MQ=255) gAAAAGGTCGGCCCGGTTACCCAGCAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGGCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGccc < 3:252721/149‑1 (MQ=255) gAAAAGGTCGGCCCGGTTACCCAGAAGCTCTGCAAGGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCATTGCTGGCATGcc < 7:69972/148‑1 (MQ=255) gAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGc < 8:65848/147‑1 (MQ=255) gCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCa > 5:197640/1‑149 (MQ=255) ccGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCAtg < 4:278787/149‑1 (MQ=255) cccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTa < 8:304079/148‑1 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTaca > 1:106866/1‑149 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTaca > 5:177041/1‑149 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTaca > 2:79225/1‑149 (MQ=255) aGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGcc > 4:254743/1‑149 (MQ=255) tACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTGTTTTCATGTGTTTAGACGgccgccgc > 4:276381/1‑147 (MQ=255) cAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCg > 2:13922/1‑148 (MQ=255) aaCGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCg > 8:27829/1‑147 (MQ=255) aaCGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCg > 7:60169/1‑147 (MQ=255) ccGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGtt > 8:2500/1‑149 (MQ=255) gTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTAc > 4:279875/1‑149 (MQ=255) tCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCcg > 3:328618/1‑149 (MQ=255) ccAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCcgc > 5:359024/1‑149 (MQ=255) agagCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCcgcg > 3:157275/1‑148 (MQ=255) cggcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAg > 8:159966/1‑149 (MQ=255) gcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc < 6:177041/149‑1 (MQ=255) gcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc > 3:170082/1‑149 (MQ=255) gcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc < 6:359024/149‑1 (MQ=255) cgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc > 8:262193/1‑148 (MQ=255) gTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCacac > 6:360525/1‑149 (MQ=255) agagGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCATTGCTGGCATGCCCCGttt > 2:103379/1‑89 (MQ=37) agagGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCca < 2:158873/144‑1 (MQ=255) agagGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCca > 1:158873/1‑144 (MQ=255) agagGCACCGGCAGGCTGGATCGTCAAGGTCGCATGAAACGCATCAGCTGTATGAAAACGGGGCATGCCATTGCTGGCATGCCCCGttt < 1:103379/89‑1 (MQ=25) ggCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCaataat < 6:147801/149‑1 (MQ=255) cTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGc < 3:148734/149‑1 (MQ=255) tGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCcgc > 7:158160/1‑118 (MQ=255) tGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCcgc < 8:158160/118‑1 (MQ=255) tGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGcc > 7:186626/1‑149 (MQ=255) gATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTg < 2:234303/149‑1 (MQ=255) aTCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTgg < 8:60169/149‑1 (MQ=255) tCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACAGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTggg < 3:95544/149‑1 (MQ=255) gTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGga < 7:159966/148‑1 (MQ=255) tCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCg > 4:306658/1‑149 (MQ=255) tCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCg > 3:320898/1‑149 (MQ=255) gCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGtt < 4:328618/149‑1 (MQ=255) ccTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAACCAATAATGCCTGGGAGATCACCg > 2:340829/1‑146 (MQ=255) ccTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAACCAATAATGCCTGGGAGATCACCGtt < 1:340829/148‑1 (MQ=255) gAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGt > 7:201932/1‑147 (MQ=255) aaaCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCa < 7:2500/148‑1 (MQ=255) aaaCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCa < 1:13922/148‑1 (MQ=255) aaCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGt < 3:254743/149‑1 (MQ=255) aCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTg < 4:320898/149‑1 (MQ=255) atgaaaaaaCGGGGCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCa < 1:24491/141‑1 (MQ=38) atgaaaaaaCGGGGCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCa > 2:24491/6‑146 (MQ=38) tGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCg < 2:106866/149‑1 (MQ=255) aaaCGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTACCggg > 8:143001/1‑148 (MQ=255) ggggCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGc < 6:197640/149‑1 (MQ=255) ggCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGccc > 2:89447/1‑149 (MQ=255) aGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATAACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCggg < 1:79225/149‑1 (MQ=255) gCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGt < 5:334374/149‑1 (MQ=255) aaTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTGa > 6:126889/1‑149 (MQ=255) aaTGGCATGCACCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGACTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTGa > 6:193816/1‑149 (MQ=255) | CACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTGA > NC_002947/3983896‑3984190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |