Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,035,604 A→G 6.5% M219V (ATG→GTG) ‡ PP_3557 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,035,6040AG 6.5% 177.6 / 2.6 62M219V (ATG→GTG) ‡PP_3557methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (25/32);  new base G (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GATGGCCCTGCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACC  >  NC_002947/4035456‑4035750
                                                                                                                                                    |                                                                                                                                                  
gATGGCCCTGCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCtga                                                                                                                                                    <  4:368511/149‑1 (MQ=255)
    gCCCTGCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGAc                                                                                                                                                >  8:167778/1‑149 (MQ=255)
    gCCCTGCCCGAAAATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCtgatga                                                                                                                                                 <  6:257655/148‑1 (MQ=255)
         gCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAACGGCCAGCCGGCCAACAGCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACGTCTACGCGATCGATGGCCAGCAGGTGCTGCTGAGCAgat                                                                                                                                           >  8:196730/1‑147 (MQ=255)
         gCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAACGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGAc                                                                                                                                                <  7:196730/144‑1 (MQ=255)
           ccGAACATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATc                                                                                                                                         <  7:201896/149‑1 (MQ=255)
                   gacatgGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTccc                                                                                                                                 >  2:244129/1‑149 (MQ=255)
                     catgGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGGGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGc                                                                                                                               <  2:293819/149‑1 (MQ=255)
                       tgGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTg                                                                                                                             <  1:244129/149‑1 (MQ=255)
                                   agaTCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGc                                                                                                                 <  6:377067/149‑1 (MQ=255)
                                     aTCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGc                                                                                                                 >  7:139148/1‑147 (MQ=255)
                                         gCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACgg                                                                                                                  <  4:13564/142‑1 (MQ=255)
                                         gCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACgg                                                                                                                  >  3:13564/1‑142 (MQ=255)
                                          ccccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCa                                                                                                          >  2:261284/1‑149 (MQ=255)
                                           cccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAt                                                                                                         >  7:154093/1‑149 (MQ=255)
                                            ccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAtt                                                                                                        <  6:350295/149‑1 (MQ=255)
                                            ccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAtt                                                                                                        <  8:138690/149‑1 (MQ=255)
                                            ccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAtt                                                                                                        >  5:350295/1‑149 (MQ=255)
                                            ccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAtt                                                                                                        <  5:218017/149‑1 (MQ=255)
                                            ccAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCAtt                                                                                                        <  1:261284/149‑1 (MQ=255)
                                                cGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCa                                                                                                    <  3:354645/149‑1 (MQ=255)
                                                  gCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCAcc                                                                                                  <  8:166194/149‑1 (MQ=255)
                                                      gCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATTGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGt                                                                                              <  7:315339/149‑1 (MQ=255)
                                                       ccGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTc                                                                                             >  3:253939/1‑149 (MQ=255)
                                                          gCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCAt                                                                                          >  3:82691/1‑149 (MQ=255)
                                                            cAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATc                                                                                         >  1:76862/1‑148 (MQ=255)
                                                            cAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATAATTGCCACCAGGTCCATc                                                                                         >  8:232594/1‑148 (MQ=255)
                                                             aaCACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGa                                                                                       >  3:46756/1‑149 (MQ=255)
                                                              acacCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGa                                                                                       >  3:3966/1‑148 (MQ=255)
                                                                acCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCa                                                                                                                      >  5:216031/1‑115 (MQ=255)
                                                                acCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCa                                                                                                                      <  6:216031/115‑1 (MQ=255)
                                                                 ccTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATc                                                                                   <  3:331570/149‑1 (MQ=255)
                                                                     gtggGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAAc                                                                                <  2:76862/148‑1 (MQ=255)
                                                                      tggGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCt                                                                              <  8:154093/149‑1 (MQ=255)
                                                                              cccACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGTTCATTGCCACCCTGTCCATCGACATCAACCTCAATAGcc                                                                      >  2:310021/1‑149 (MQ=255)
                                                                               ccACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGAc                                                                                                                                                <  1:309422/74‑1 (MQ=255)
                                                                               ccACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGAc                                                                                                                                                >  2:309422/1‑74 (MQ=255)
                                                                               ccACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGcc                                                                      >  4:283010/1‑148 (MQ=255)
                                                                               ccACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCt                                                                     >  5:273598/1‑149 (MQ=255)
                                                                               ccACGCACCTCCGGCAAGGTGCGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCt                                                                     >  6:26266/1‑149 (MQ=255)
                                                                                cACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTg                                                                    >  6:249959/1‑149 (MQ=255)
                                                                                 aCGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTg                                                                    >  2:49918/1‑148 (MQ=255)
                                                                                   gCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAg                                                                 <  4:253939/149‑1 (MQ=255)
                                                                                               aGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCag                                                     <  4:46756/149‑1 (MQ=255)
                                                                                                 gtgtGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCagga                                                   <  7:19673/149‑1 (MQ=255)
                                                                                                  tgtgCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCaggag                                                  <  5:249959/149‑1 (MQ=255)
                                                                                                             gagCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCa                                        <  7:232594/148‑1 (MQ=255)
                                                                                                              agCCGAACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGc                                      <  7:167778/149‑1 (MQ=255)
                                                                                                                ccGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCct                                    >  4:178214/1‑149 (MQ=255)
                                                                                                                  gTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCctct                                  <  4:82691/149‑1 (MQ=255)
                                                                                                                   tACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTa                                 <  4:66543/149‑1 (MQ=255)
                                                                                                                     cTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACg                               <  5:26266/149‑1 (MQ=255)
                                                                                                                      ttCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACg                               >  7:254410/1‑148 (MQ=255)
                                                                                                                                    tGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAg                            >  6:364656/1‑137 (MQ=255)
                                                                                                                                    tGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAg                            <  5:364656/137‑1 (MQ=255)
                                                                                                                                      gCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGTTCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTc              <  1:310021/149‑1 (MQ=255)
                                                                                                                                       ccAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCg             <  6:273598/149‑1 (MQ=255)
                                                                                                                                       ccAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCACAGCCTCTACGAAGGCCGCACCACGGTCg             <  8:139148/149‑1 (MQ=255)
                                                                                                                                         agcagGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGc           <  3:283010/149‑1 (MQ=255)
                                                                                                                                             gatGCTGGTCATCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCaa                                                                           <  3:158139/79‑1 (MQ=255)
                                                                                                                                             gatGCTGGTCATCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCaa                                                                           <  3:152450/79‑1 (MQ=255)
                                                                                                                                             gatGCTGGTCATCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCaa                                                                           >  4:158139/3‑81 (MQ=255)
                                                                                                                                             gatGCTGGTCATCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCaa                                                                           >  4:152450/3‑81 (MQ=255)
                                                                                                                                                gCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCa    >  6:243089/1‑149 (MQ=255)
                                                                                                                                                  tgatgaCCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCg             >  4:90521/1‑138 (MQ=255)
                                                                                                                                                  tgatgaCCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCg             <  3:90521/138‑1 (MQ=255)
                                                                                                                                                  tgatgaCCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCAcc  >  5:187253/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GATGGCCCTGCCCGAACATGACATGGCCAACACCGAGATCGGCCCCAGCGGCCAGCCGGCCAACACCTGGTGGGTATGCCCACGCACCTCCGGCAAGGTGTGCGTGGTAGAGCCGTACTTCTACGACATCGATGGCCAGCAGGTGCTGATGACCAGCATCGTGTTCCCGCTGGCGGTCAACGGCAAGATCATTGCCACCCTGTCCATCGACATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACC  >  NC_002947/4035456‑4035750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑