Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,155,102 G→A 7.3% T131M (ACG→ATG)  PP_3658 ← aromatic compound MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,155,1020GA 7.3% 155.1 / 2.5 55T131M (ACG→ATG) PP_3658aromatic compound MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

ACGTAAAACACATATTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCG  >  NC_002947/4154955‑4155250
                                                                                                                                                   |                                                                                                                                                     
aCGTAAAACACATATTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCg                                                                                                                                                       >  3:126294/1‑148 (MQ=255)
 cGTAAAACACATATTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTa                                                                                                                                                     <  3:89808/149‑1 (MQ=255)
        cacaTATTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATgg                                                                                                                                              <  8:2575/149‑1 (MQ=255)
            tatTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGTACGGCGCAGATgggtga                                                                                                                                          <  2:52549/149‑4 (MQ=255)
                   aaCCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑Cg                                                                                                                                  <  7:58886/149‑1 (MQ=255)
                       gTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATCGTCACCAGCCGGGTGCTAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCaa                                                                                                                              <  7:80830/149‑1 (MQ=255)
                               cGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCaa                                                                                                                       <  8:329079/148‑1 (MQ=255)
                               agATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACCA‑CGCCAATTCCCAAg                                                                                                                      >  7:342473/2‑149 (MQ=255)
                                gATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGc                                                                                                                     >  5:226990/1‑149 (MQ=255)
                                 aTCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGcc                                                                                                                    >  7:135233/1‑149 (MQ=255)
                                 aTCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCACACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAg                                                                                                                      >  8:275088/1‑147 (MQ=255)
                                     gttgttTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGc                                                                                                                 >  1:97782/1‑148 (MQ=255)
                                     gttgttTACCCGTCAGAGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGc                                                                                                                 >  8:246669/1‑148 (MQ=255)
                                      ttgttTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGa                                                                                                               >  6:362218/1‑149 (MQ=255)
                                       tgttTACCCGTCAGCGCTACCAGAATTCCGCCAAGCGATTAGACTGCGAAAACGATTGTCACGAGACTGGTGCGAACTTTCAAGGGCGAGAACTCTCCCTTCTGCGCCGTACAGATTGGCAACTCAC‑CGCCAATTCCCAATCCGGCGa                                                                                                               <  3:270581/148‑1 (MQ=255)
                                        gttTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGAt                                                                                                              >  8:310918/1‑148 (MQ=255)
                                             cccGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAgcg                                                                                                        <  4:126294/149‑1 (MQ=255)
                                                gTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCAt                                                                                                     <  7:246669/149‑1 (MQ=255)
                                                    gcgcTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATa                                                                                                 <  7:275088/149‑1 (MQ=255)
                                                          gcAGAATTCCGCACACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGTGCAACACAC‑CGCCAATTCCCAAGCCGGCGATa                                                                                                             <  5:301941/130‑1 (MQ=255)
                                                          ccAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAg                                                                                           <  6:226990/149‑1 (MQ=255)
                                                           cAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGc                                                                                                                                 <  4:315125/110‑1 (MQ=255)
                                                           cAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGc                                                                                                                                 >  3:315125/1‑110 (MQ=255)
                                                             gAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGt                                                                                         >  4:245180/1‑148 (MQ=255)
                                                                ttCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCATACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGc                                                                                     >  7:274100/1‑149 (MQ=255)
                                                                    gCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGTGc                                                                                 <  6:99265/149‑1 (MQ=255)
                                                                    gCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGc                                                                                 >  2:120246/1‑149 (MQ=255)
                                                                       cACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAAGTCACCCATCTGCGCCATACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCTGGCTGt                                                                              <  8:274100/149‑1 (MQ=255)
                                                                        aCCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACACATCTGCGCCGTACAGATGGGCAACACGC‑CGCCAATTCCCAAGCCGGCGACAAAGCGCATGATACTGAAGGTCAGCGGGCTgg                                                                              >  8:59160/1‑147 (MQ=255)
                                                                            aaTAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGt                                                                          >  3:167375/1‑148 (MQ=255)
                                                                                 ccTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAAccc                                                                    >  8:229329/1‑149 (MQ=255)
                                                                                 ccTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAAccc                                                                    >  5:269338/1‑149 (MQ=255)
                                                                                     cGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGcc                                                                <  8:135233/149‑1 (MQ=255)
                                                                                     cGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGc                                                                 <  5:362218/148‑1 (MQ=255)
                                                                                             aTGGTCACAAGCCGGGTGCGACCTTTCAAGGGCGAGAACTCACCCATCTGCGCCATACAGATGGGCAACACAC‑CGCCAATTCCGAAGCCGGCGATAAAGCGCATGCTACTGAAGGTCAGCGGGCTCTCAGTGAACCCTGCCGCAGCGGt                                                        >  5:107119/1‑149 (MQ=255)
                                                                                                   aCCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCATTTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAAc                                                  <  8:122125/149‑1 (MQ=255)
                                                                                                   aCCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAAc                                                  <  2:97782/149‑1 (MQ=255)
                                                                                                   aCCAGCCGCGTCCGAACTATCAAGGGCGCGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGCGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAAc                                                  >  2:94759/1‑149 (MQ=255)
                                                                                                        ccGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCggg                                                                                  >  7:69175/1‑112 (MQ=255)
                                                                                                        ccGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCggg                                                                                  <  8:69175/112‑1 (MQ=255)
                                                                                                             tGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAgc                                        <  6:269338/149‑1 (MQ=255)
                                                                                                                 aaCTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGGCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTa                                    <  5:241356/149‑1 (MQ=255)
                                                                                                                    tttCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTaca                                  >  5:316432/1‑148 (MQ=255)
                                                                                                                     ttCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTaca                                  >  8:30142/1‑147 (MQ=255)
                                                                                                                      tCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGTGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTaca                                  >  4:135804/1‑146 (MQ=255)
                                                                                                                           ggCTAGAACTCACCCAGCTGCGCCATACAGTGGGGCAACACAC‑CGCCAATTCCCAAGGCGGCTATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATcgc                          <  6:107119/149‑1 (MQ=255)
                                                                                                                             cGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGa                        <  3:245180/149‑1 (MQ=255)
                                                                                                                             cGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGa                        <  7:310918/149‑1 (MQ=255)
                                                                                                                                    tCACCCATCTGCGCCGTACAGATGGGCAACACACTCGCCATTTCCCACGCCGGCGTTAAAGCGCTTGATAGTGAGGGTCGGCGGGCTGACAGTGAGCCCTGCCGCAGCGGTGAAACCCCTGACCCGCGCTCCACGGAACGCGAGCAttt                  >  1:241592/1‑149 (MQ=255)
                                                                                                                                      aCCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTcg               >  7:52862/1‑149 (MQ=255)
                                                                                                                                            cTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCAAGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGAAGAACAGCGCTACACAGATCGCGATCATTTTcggccgga         >  5:74820/1‑148 (MQ=255)
                                                                                                                                              gcgcCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAAGCCTGCCGCTGCGGTGCATACGGTGAACAGCGCTACACAGCTCGCGATCATTTTcggccgg          >  6:364360/1‑146 (MQ=255)
                                                                                                                                               cgcCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTcggccgg          <  1:120246/145‑1 (MQ=255)
                                                                                                                                                gcCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAAt     >  6:33967/1‑149 (MQ=255)
                                                                                                                                                  cGTGCAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCt                                               >  5:146752/1‑105 (MQ=255)
                                                                                                                                                  cGTGCAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCt                                               <  6:146752/105‑1 (MQ=255)
                                                                                                                                                   gTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCg  <  7:229329/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACGTAAAACACATATTGCCAACCGTAGCTTTCGATCAGTTGTTTACCCGTCAGCGCTACCAGAATTCCGCCCACCGAATAGCCTGCGAAAACGATGGTCACCAGCCGGGTGCGAACTTTCAAGGGCGAGAACTCACCCATCTGCGCCGTACAGATGGGCAACACAC‑CGCCAATTCCCAAGCCGGCGATAAAGCGCATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCG  >  NC_002947/4154955‑4155250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑