Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,294 T→C 6.5% intergenic (+136/+233) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,2940TC 6.5% 173.7 / 2.6 61intergenic (+136/+233)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/23);  new base C (2/2);  total (36/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

CAGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGC  >  NC_002947/4196146‑4196436
                                                                                                                                                        |                                                                                                                                              
cAGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGgt                                                                                                                                                <  6:69971/149‑1 (MQ=255)
  gggCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGCCAAGCTAGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGgtgt                                                                                                                                              >  6:202297/1‑149 (MQ=255)
  gggCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGgtgt                                                                                                                                              <  2:53238/149‑1 (MQ=255)
      cTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGAc                                                                                                                                          >  1:71264/1‑149 (MQ=255)
      cTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGAc                                                                                                                                          <  2:71264/149‑1 (MQ=255)
          tGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGtct                                                                                                                                      <  5:41179/149‑1 (MQ=255)
              ggTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGc                                                                                                                                  >  4:372457/1‑149 (MQ=255)
              ggTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGc                                                                                                                                  <  4:236610/149‑1 (MQ=255)
                  aGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGACGT‑CGACACCGTCGACGGCTCggttcac                                                                                                                              <  1:41489/149‑6 (MQ=255)
                      aCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                          >  4:154175/1‑149 (MQ=255)
                          cTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAg                                                                                                                       <  5:247483/148‑1 (MQ=255)
                          cTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGc                                                                                                                      >  2:266611/1‑149 (MQ=255)
                             gCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCAt                                                                                                                   >  3:129713/1‑149 (MQ=255)
                                 cTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCTTGCCCTATGCGCCCGG‑CTGT‑‑CGTGTACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                 <  7:320532/147‑1 (MQ=255)
                                 cTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGt                                                                                                               >  6:129631/1‑149 (MQ=255)
                                 cTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGt                                                                                                               >  3:262544/1‑149 (MQ=255)
                                    ctctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCAc                                                                                                            >  5:262411/1‑149 (MQ=255)
                                    ctctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGCCAc                                                                                                            <  8:253890/149‑1 (MQ=255)
                                     tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                 >  8:2576/1‑143 (MQ=255)
                                     tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                 <  7:2576/143‑1 (MQ=255)
                                      ctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGc                                                                                                          >  2:264232/1‑149 (MQ=255)
                                       tACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGGCCTCAGGCACGCt                                                                                                         >  6:31062/1‑149 (MQ=255)
                                                aggGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGccc                                                                                                <  6:311865/149‑1 (MQ=255)
                                                      aTGGCCCGACAAGCTCGCATTAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTg                                                                                          <  6:262411/149‑1 (MQ=255)
                                                      aTGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACtt                                                                                           <  5:129631/148‑1 (MQ=255)
                                                       tGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGc                                                                                         <  4:262544/149‑1 (MQ=255)
                                                         gCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCt                                                                                       >  1:65729/1‑149 (MQ=255)
                                                         gCCCGAAAAGCTCGCATGAGATCGCCTAGCAGATCATGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGCTGTCGACGTCTCGGCCCTCCGGGCAGCCATAAGACACGCTTGCGCGCCCCACTTGCCt                                                                                       >  6:286751/1‑149 (MQ=39)
                                                           ccGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgc                                                                                     <  4:129713/149‑1 (MQ=255)
                                                                  gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGa                                                                               >  3:43473/1‑148 (MQ=255)
                                                                      gCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGTGGCGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAAt                                                                            >  1:293535/1‑149 (MQ=255)
                                                                      gCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTc                                                                          >  5:102362/1‑149 (MQ=255)
                                                                       cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                         >  3:78457/1‑149 (MQ=255)
                                                                            gaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACa                                                                    >  4:88388/1‑149 (MQ=255)
                                                                              tCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATg                                                                  <  1:264232/149‑1 (MQ=255)
                                                                                  cTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGctct                                                              >  7:293560/1‑149 (MQ=255)
                                                                                          tCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGTCTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                        >  1:237653/1‑116 (MQ=255)
                                                                                          tCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                        <  2:237653/115‑1 (MQ=255)
                                                                                               cTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCa                                                 >  4:84477/1‑149 (MQ=255)
                                                                                                tAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                >  7:191943/1‑149 (MQ=255)
                                                                                                 aaGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCTGCAGAACTTCTGCACATGCTCGGGTCGAGCGCGCagg                                               >  3:276899/1‑147 (MQ=37)
                                                                                                 aaGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                               >  3:89174/1‑149 (MQ=255)
                                                                                                  aGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                               <  4:89174/148‑1 (MQ=255)
                                                                                                    gACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCCCTTGCCTGCAGCAGAAATTCTGAACATGCTCTGGGGGGCCGCGCATgggt                                            >  8:312396/1‑147 (MQ=38)
                                                                                                       tGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtgc                                         >  3:351685/1‑149 (MQ=255)
                                                                                                              ccTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                  >  6:6966/1‑149 (MQ=255)
                                                                                                                tATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGGAGCAGCAATTCTGACCATGCTCTGTGGGCCCGGTCCTGGATTGCTGCggtggg                                >  8:322139/1‑144 (MQ=37)
                                                                                                                 aTGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTggg                               >  7:23498/1‑149 (MQ=255)
                                                                                                                  tGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCGATCAGTCACGCTTGCGCGCCCGACTTGCCTGCTGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                <  5:286751/147‑1 (MQ=255)
                                                                                                                  tGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTggg                               <  3:154175/148‑1 (MQ=255)
                                                                                                                  tGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                <  5:140382/147‑1 (MQ=255)
                                                                                                                    ggCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTa                             <  3:372457/148‑1 (MQ=255)
                                                                                                                      cccGG‑CTGT‑‑CGTGAACCGAGCCGTGCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTAt                            >  7:51775/1‑148 (MQ=255)
                                                                                                                      cccGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGa                          <  3:88388/149‑1 (MQ=255)
                                                                                                                          g‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCa                      <  8:51775/149‑1 (MQ=255)
                                                                                                                             tGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGGTGCGTTTGGGTATGACTCAg                     >  6:219525/1‑148 (MQ=255)
                                                                                                                             tGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAg                     >  5:67733/1‑148 (MQ=255)
                                                                                                                                   gTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCatgccta                >  5:5064/1‑143 (MQ=255)
                                                                                                                                   gTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                <  4:147041/149‑1 (MQ=255)
                                                                                                                                   gTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                <  5:202297/149‑1 (MQ=255)
                                                                                                                                         cGAGACGT‑CGACACCGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGc          >  5:195818/1‑149 (MQ=255)
                                                                                                                                          gagaCGT‑CGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGt                              <  6:259171/128‑1 (MQ=255)
                                                                                                                                          gagaCGT‑CGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTAGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGt                              >  5:259171/1‑128 (MQ=255)
                                                                                                                                                   gACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGc  >  4:105127/1‑148 (MQ=255)
                                                                                                                                                        |                                                                                                                                              
CAGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGG‑CTGT‑‑CGTGAACCGAGCCGT‑CGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGC  >  NC_002947/4196146‑4196436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑